LOCUS       V027796                 8182 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027796
VERSION     V027796
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             635..3232
                     /label="Mlxipl(NM_001393706)"
                     /note="Mlxipl(NM_001393706)"
                     /gene="Mlxipl"
     CDS             3245..3310
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3351..3575
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     promoter        3591..4114
                     /label="mCMV promoter"
     CDS             4128..4847
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4970..5091
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5098..5553)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5580..5684
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5686..6043
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6078..6872
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7104..7151
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7480..8068
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct ccgaattcgc caccatggcg cgcgcgctgg cggatctatc
      661 cgtgaacttg caggtccccc gggtcgtccc cagcccggat tcggactcgg atacagactt
      721 ggaggatccg agtccccggc gcagcgcggg tggcctgcat cgatcacagg tcattcacag
      781 cggacacttc atggtgtcct cgccgcacag cgactcgctg acccggcgac gcgaccagga
      841 ggggcccgtg gggctcgccg acttcgggcc gcgcagcatc gatccgacac tcacccgcct
      901 cttcgaatgc ttgagcctgg cttacagcgg caagctggtg tctcccaagt ggaagaactt
      961 caaaggcctc aagttgctat gccgggacaa gattcggctg aacaacgcca tctggagagc
     1021 ctggtacatt cagtatgtgc aacggaggaa gagcccagta tgtggcttcg taactcctct
     1081 gcaggggtca gaagcagatg agcaccggaa acctgaggct gttgtcttgg agggtaatta
     1141 ctggaagcgg cgcatcgagg tggtgatgcg tgaataccac aagtggagaa tctactacaa
     1201 gaagcggctc cgtaagtcca gcagggaagg ggatttcctg gctcccaagc aggtggaagg
     1261 tgggtggccg ccaccagaga gatggtgcga acagctcttc tccagcgtgg tgcctgtgct
     1321 gcttgggggc tccgaggagg agcctggggg ccggcagctt ctcgacttgg actgcttctt
     1381 gtccgatatc tccgacacac tcttcaccat gacacagccc agcccctcgt ccctgcagct
     1441 gccctcagaa gacgcttatg ttggcaatgc tgacatgatc cagccagacc tgacgccgct
     1501 gcagcccagc ctggatgact tcatggagat atcagatttc ttcaccaatt atcgcccccc
     1561 tcagacgccc acatcttcaa acttcccaga gccccccagc tttggcccca tggctgactc
     1621 cctcttcagc ggtgggatcc tgggcccaga gatgccatct ccagcctccg cttcctcctc
     1681 ctcggggatg acccctctct cagggaatac acgtctccag gctcgaaaca gctgctctgg
     1741 acccttggat cccagcacct ttccgagctc tgaattcctt cttcctgaag acccaaagac
     1801 caagatgcca cctgctcctg taccgactcc tctccttccg taccccggcc ctgtcaaggt
     1861 gcatggctta gagccctgca ctccatctcc cttccccacg atggctccac ctcctgcttt
     1921 gttgtcagaa gagcctctct tctctgcaag gttccccttc accacagtcc ctcccgctcc
     1981 aggagtgtct acccttcctg cacccacaac ctttgttccc acgccacagc ctggccctgt
     2041 ccctggccct gtccctttcc ccgtagacca tctgccccat gggtacctgg aacctgtctt
     2101 tgggcctcac ttcacggtgc ctcagggtgt gcagcccaga tgcaagccct gctctccacc
     2161 tcctggggga cgaaaagcca gtccacccac cttgacctct gccactgcca gccccacggc
     2221 cactgctact gccagagaca acaacccctg ccttacacag ctgctcaggg cagccaagcc
     2281 tgaacaagta ctggaacctt ccactgtgcc cagcaccctc ctccggcctc cagagtcccc
     2341 gcaggatgca gtccctgaaa tcccccgtgt ccgtgcattt taccccccaa tcccagcccc
     2401 tacaccacct aggccacctc caggccctgc cacattggct cctcccaggt ccctggttgt
     2461 ccccaaagca gagaggctct cacctccagc atccagcggc agtgagcggc gactatcagg
     2521 ggatctcaac tccattcaac ccccgggggc actgagtgtc cacctgtctc cccctcaaac
     2581 tgtcctaagc cggggtcgtg tagacaacaa caagatggag aaccggcgaa tcacacacat
     2641 ctccgcagag cagaagaggc gtttcaatat taagctagga tttgacactc tgcacggact
     2701 tgtcagcacg ctcagtgccc agcccagcct caaggtgagc aaagcaacca cgcttcagaa
     2761 gacggcggag tacatcctga tgctacagca ggagcgggcg gccatgcagg aggaagcgca
     2821 gcaactgagg gatgaaatag aggagctcaa tgctgccatc aacttgtgcc agcagcagct
     2881 accggccacc ggggtgccca tcacacatca gcgctttgac cagatgcggg acatgtttga
     2941 tgactatgtc cggacccgca cgctgcacaa ctggaagttc tgggtattca gcatcctcat
     3001 ccgaccttta tttgagtcct tcaatgggat ggtgtctacc gcaagcttgc acagcctccg
     3061 ccagacctca ctggcatggc tggaccagta ctgttccctg cctgctctcc ggccaactgt
     3121 cctgaattcc cttcgccagc tcagcacttc caccagcatc ctgactgacc cgagcctcgt
     3181 gcctgagcaa gccacaaggg cagtcacaga gggtcccctg ggcagaccat taaagcttac
     3241 gcgtgactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     3301 cgatgacaag tgaggatccg ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     3361 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3421 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     3481 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     3541 tagcaggcat gctggggatg cggtgggctc tatggtctag agatatactg agtcattagg
     3601 gactttccaa tgggttttgc ccagtacata aggtcaatag gggtgaatca acaggaaagt
     3661 cccattggag ccaagtacac tgagtcaata gggactttcc attgggtttt gcccagtaca
     3721 aaaggtcaat agggggtgag tcaatgggtt tttcccatta ttggcacgta cataaggtca
     3781 ataggggtga gtcattgggt ttttccagcc aatttaatta aaacgccatg tactttccca
     3841 ccattgacgt caatgggcta ttgaaactaa tgcaacgtga cctttaaacg gtactttccc
     3901 atagctgatt aatgggaaag taccgttctc gagccaatac acgtcaatgg gaagtgaaag
     3961 ggcagccaaa acgtaacacc gccccggttt tcccctggaa attccatatt ggcacgcatt
     4021 ctattggctg agctgcgttc tacgtgggta taagaggcgc gaccagcgtc ggtaccgtcg
     4081 cagtcttcgg tctgaccacc gtagaacgca gatcaccggt cgccaccatg gtgagcaagg
     4141 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     4201 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4261 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4321 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4381 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4441 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4501 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4561 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     4621 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     4681 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     4741 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     4801 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact
     4861 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     4921 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     4981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     5101 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     5161 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     5221 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     5281 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     5341 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5401 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     5461 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     5521 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     5581 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5641 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     5701 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5761 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5881 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5941 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6001 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     6061 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6121 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6181 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6241 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6301 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6361 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6421 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6481 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6541 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6601 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6661 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6721 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6781 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6841 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     6901 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     6961 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     7021 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     7081 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     7141 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     7201 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     7261 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     7321 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7381 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7441 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7501 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7561 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7621 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7681 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8041 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8101 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8161 taaccgtatt accgccatgc at
//
