LOCUS       V043505                 7822 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043505
VERSION     V043505
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             635..2950
                     /label="NFE2L1(NM_003204)"
                     /note="NFE2L1(NM_003204)"
                     /gene="NFE2L1"
     polyA_signal    2991..3215
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     promoter        3231..3754
                     /label="mCMV promoter"
     CDS             3768..4487
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4610..4731
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4738..5193)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5220..5324
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5326..5683
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5718..6512
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6744..6791
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7120..7708
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct ccgaattcgc caccatgctt tctctgaaga aatacttaac
      661 ggaaggactt ctccagttca ccattctgct gagtttgatt ggggtacggg tggacgtgga
      721 tacttacctg acctcacagc ttcccccact ccgggagatc atcctggggc ccagttctgc
      781 ctatactcag acccagttcc acaacctgag gaataccttg gatggctatg gtatccaccc
      841 caagagcata gacctggaca attacttcac tgcccggcgg ctcctcagtc aggtgagggc
      901 cctggacagg ttccaggtgc caaccactga ggtaaatgcc tggctggttc accgagaccc
      961 agaggggtct gtctctggca gtcagcccaa ctcaggcctc gccctcgaga gttccagtgg
     1021 cctccaagat gtgacaggcc cagacaacgg ggtgcgagaa agcgaaacgg agcagggatt
     1081 cggtgaagat ttggaggatt tgggggctgt agccccccca gtcagtggag acttaaccaa
     1141 agaggacata gatctgattg acatcctttg gcgacaggat attgatctgg gggctgggcg
     1201 tgaggttttt gactatagtc accgccagaa ggagcaggat gtggagaagg agctgcgaga
     1261 tggaggcgag caggacacct gggcaggcga gggcgcggaa gctctggcac ggaacctgct
     1321 agtggatgga gagactgggg agagcttccc tgcacaggtg cctagtgggg aggaccagac
     1381 ggccctgtcc ctggaagagt gccttaggct gctggaagcc acctgcccct ttggggagaa
     1441 tgctgagttt ccagcagaca tttccagcat aacagaagca gtgcctagtg agagtgagcc
     1501 ccctgctctt caaaacaacc tcttgtctcc tcttctgacc gggacagagt caccatttga
     1561 tttggaacag cagtggcaag atctcatgtc catcatggaa atgcaggcca tggaagtgaa
     1621 cacatcagca agtgaaatcc tgtacagtgc ccctcctgga gacccactga gcaccaacta
     1681 cagccttgcc cccaacactc ccatcaatca gaatgtcagc ctgcatcagg cgtccctggg
     1741 gggctgcagc caggacttct tactcttcag ccccgaggtg gaaagcctgc ctgtggccag
     1801 tagctccacg ctgctcccgt tggcccccag caattctacc agcctcaact ccaccttcgg
     1861 ctccaccaac ctgacagggc tcttctttcc accccagctc aatggcacag ccaatgacac
     1921 agcaggccca gagctgcctg accctttggg gggtctgtta gatgaagcta tgttggatga
     1981 gatcagcctt atggacctgg ccattgaaga aggctttaac cctgtgcagg cctcccagct
     2041 ggaggaggaa tttgactctg actcaggcct ttccttagac tcgagccata gcccttcttc
     2101 cctaagcagc tctgaaggca gttcttcctc ttcttcctcc tcctcttcct cttcttcctc
     2161 tgcttcttcc tctgcctctt cctccttttc tgaggaaggt gcggttggct acagctctga
     2221 ctctgagacc ctggatctgg aagaggccga gggtgctgtg ggctaccagc ctgagtattc
     2281 caagttctgc cgcatgagct accaggatcc agctcagctc tcatgcctgc cctacctgga
     2341 gcacgtgggc cacaaccaca catacaacat ggcacccagt gccctggact cagccgacct
     2401 gccaccaccc agtgccctca agaaaggcag caaggagaag caggctgact tcctggacaa
     2461 gcagatgagc cgggatgagc accgagcccg agccatgaag atccctttca ccaatgacaa
     2521 aatcatcaac ctgcctgtgg aggagttcaa tgaactgctg tccaaatacc agttgagtga
     2581 agcccagctg agcctcatcc gagacatccg gcgccggggc aagaacaaga tggcggcgca
     2641 gaactgccgc aagcgcaagc tggacaccat cctgaatctg gagcgtgatg tggaggacct
     2701 gcagcgtgac aaagcccggc tgctgcggga gaaagtggag ttcctgcgct ccctgcgaca
     2761 gatgaagcag aaggtccaga gcctgtacca ggaggtgttt gggcggctgc gagatgagaa
     2821 cggacgaccc tactcgccca gtcagtatgc gctccagtac gccggggacg gcagtgtcct
     2881 cctcatcccc cgcacgatgg ccgaccagca ggcccggcgg caggagagga agccaaagga
     2941 ccggagaaag tgaggatccg ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     3001 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3061 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     3121 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     3181 tagcaggcat gctggggatg cggtgggctc tatggtctag agatatactg agtcattagg
     3241 gactttccaa tgggttttgc ccagtacata aggtcaatag gggtgaatca acaggaaagt
     3301 cccattggag ccaagtacac tgagtcaata gggactttcc attgggtttt gcccagtaca
     3361 aaaggtcaat agggggtgag tcaatgggtt tttcccatta ttggcacgta cataaggtca
     3421 ataggggtga gtcattgggt ttttccagcc aatttaatta aaacgccatg tactttccca
     3481 ccattgacgt caatgggcta ttgaaactaa tgcaacgtga cctttaaacg gtactttccc
     3541 atagctgatt aatgggaaag taccgttctc gagccaatac acgtcaatgg gaagtgaaag
     3601 ggcagccaaa acgtaacacc gccccggttt tcccctggaa attccatatt ggcacgcatt
     3661 ctattggctg agctgcgttc tacgtgggta taagaggcgc gaccagcgtc ggtaccgtcg
     3721 cagtcttcgg tctgaccacc gtagaacgca gatcaccggt cgccaccatg gtgagcaagg
     3781 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3841 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3901 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3961 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4021 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4081 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4141 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4201 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     4261 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     4321 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     4381 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     4441 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact
     4501 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     4561 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     4621 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4681 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     4741 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4801 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     4861 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     4921 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4981 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5041 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     5101 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     5161 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     5221 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5281 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     5341 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5401 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5461 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5521 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5581 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5641 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     5701 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     5761 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5821 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5881 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     5941 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6001 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6061 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6121 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6181 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6241 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6301 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6361 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6421 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6481 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     6541 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     6601 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6661 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     6721 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     6781 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     6841 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     6901 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     6961 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7021 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7081 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7141 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7201 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7261 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7321 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7381 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7441 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7501 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7561 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7621 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7681 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7741 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7801 taaccgtatt accgccatgc at
//
