LOCUS       V028101                 7382 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028101
VERSION     V028101
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1378..3966
                     /label="ADAM15(NM_207197)"
                     /note="ADAM15(NM_207197)"
                     /gene="ADAM15"
     CDS             3973..4038
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    4170..4291
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4298..4753)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4780..4884
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4886..5243
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5278..6072
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6304..6351
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6680..7268
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tggaggtgga ggtagtggag gaggcggaag caagcttatg
     1381 cggctggcgc tgctctgggc cctggggctc ctgggcgcgg gcagccctct gccttcctgg
     1441 ccgctcccaa atataggtgg cactgaggag cagcaggcag agtcagagaa ggccccgagg
     1501 gagcccttgg agccccaggt ccttcaggac gatctcccaa ttagcctcaa aaaggtgctt
     1561 cagaccagtc tgcctgagcc cctgaggatc aagttggagc tggacggtga cagtcatatc
     1621 ctggagctgc tacagaatag ggagttggtc ccaggccgcc caaccctggt gtggtaccag
     1681 cccgatggca ctcgggtggt cagtgaggga cacactttgg agaactgctg ctaccaggga
     1741 agagtgcggg gatatgcagg ctcctgggtg tccatctgca cctgctctgg gctcagaggc
     1801 ttggtggtcc tgaccccaga gagaagctat accctggagc aggggcctgg ggaccttcag
     1861 ggtcctccca ttatttcgcg aatccaagat ctccacctgc caggccacac ctgtgccctg
     1921 agctggcggg aatctgtaca cactcagaag ccaccagagc accccctggg acagcgccac
     1981 attcgccgga ggcgggatgt ggtaacagag accaagactg tggagttggt gattgtggct
     2041 gatcactcgg aggcccagaa ataccgggac ttccagcacc tgctaaaccg cacactggaa
     2101 gtggccctct tgctggacac attcttccgg cccctgaatg tacgagtggc actagtgggc
     2161 ctggaggcct ggacccagcg tgacctggtg gagatcagcc caaacccagc tgtcaccctc
     2221 gaaaacttcc tccactggcg cagggcacat ttgctgcctc gattgcccca tgacagtgcc
     2281 cagctggtga ctggtacttc attctctggg cctacggtgg gcatggccat tcagaactcc
     2341 atctgttctc ctgacttctc aggaggtgtg aacatggacc actccaccag catcctggga
     2401 gtcgcctcct ccatagccca tgagttgggc cacagcctgg gcctggacca tgatttgcct
     2461 gggaatagct gcccctgtcc aggtccagcc ccagccaaga cctgcatcat ggaggcctcc
     2521 acagacttcc taccaggcct gaacttcagc aactgcagcc gacgggccct ggagaaagcc
     2581 ctcctggatg gaatgggcag ctgcctcttc gaacggctgc ctagcctacc ccctatggct
     2641 gctttctgcg gaaatatgtt tgtggagccg ggcgagcagt gtgactgtgg cttcctggat
     2701 gactgcgtcg atccctgctg tgattctttg acctgccagc tgaggccagg tgcacagtgt
     2761 gcatctgacg gaccctgttg tcaaaattgc cagctgcgcc cgtctggctg gcagtgtcgt
     2821 cctaccagag gggattgtga cttgcctgaa ttctgcccag gagacagctc ccagtgtccc
     2881 cctgatgtca gcctagggga tggcgagccc tgcgctggcg ggcaagctgt gtgcatgcac
     2941 gggcgttgtg cctcctatgc ccagcagtgc cagtcacttt ggggacctgg agcccagccc
     3001 gctgcgccac tttgcctcca gacagctaat actcggggaa atgcttttgg gagctgtggg
     3061 cgcaacccca gtggcagtta tgtgtcctgc acccctagag atgccatttg tgggcagctc
     3121 cagtgccaga caggtaggac ccagcctctg ctgggctcca tccgggatct actctgggag
     3181 acaatagatg tgaatgggac tgagctgaac tgcagctggg tgcacctgga cctgggcagt
     3241 gatgtggccc agcccctcct gactctgcct ggcacagcct gtggccctgg cctggtgtgt
     3301 atagaccatc gatgccagcg tgtggatctc ctgggggcac aggaatgtcg aagcaaatgc
     3361 catggacatg gggtctgtga cagcaacagg cactgctact gtgaggaggg ctgggcaccc
     3421 cctgactgca ccactcagct caaagcaacc agctccctga ccacagggct gctcctcagc
     3481 ctcctggtct tattggtcct ggtgatgctt ggtgccagct actggtaccg tgcccgcctg
     3541 caccagcgac tctgccagct caagggaccc acctgccagt acagggcagc ccaatctggt
     3601 ccctctgaac ggccaggacc tccgcagagg gccctgctgg cacgaggcac taagcaggct
     3661 agtgctctca gcttcccggc ccccccttcc aggccgctgc cgcctgaccc tgtgtccaag
     3721 agactccagg ctgagctggc tgaccgaccc aatcccccta cccgccctct gcccgctgac
     3781 ccggtggtga gaagcccgaa gtctcagggg ccagccaagc ccccaccccc aaggaagcca
     3841 ctgcctgccg acccccaggg ccggtgccca tcgggtgacc tgcccggccc aggggctgga
     3901 atcccgcccc tagtggtacc ctccagacca gcgccaccgc ctccgacagt gtcctcgctc
     3961 tacctcctcg aggactacaa agaccatgac ggtgattata aagatcatga catcgactac
     4021 aaggatgacg atgacaagta ggatccaccg gatctagata actgatcata atcagccata
     4081 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     4141 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     4201 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4261 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg
     4321 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     4381 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     4441 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     4501 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     4561 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     4621 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     4681 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     4741 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4861 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     4921 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4981 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5041 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5101 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5161 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     5221 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     5281 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5341 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5401 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     5461 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5521 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5581 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5641 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5701 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5761 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     5821 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5881 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5941 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6001 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6061 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     6121 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     6181 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6241 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     6301 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     6361 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     6421 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     6481 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     6541 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6601 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6661 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6721 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6781 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6841 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6901 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6961 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7021 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7081 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7141 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7201 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7261 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7321 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
     7381 at
//
