LOCUS       V037191                 7368 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037191
VERSION     V037191
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      595..604
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             604..669
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             670..3972
                     /label="Acly(NM_001199296)"
                     /note="Acly(NM_001199296)"
                     /gene="Acly"
     CDS             3982..4008
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
                     /transl_table=1
                     /translation="LPPLERLTL"
                     /label="NES"
     polyA_signal    4156..4277
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4284..4739)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4766..4870
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4872..5229
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5264..6058
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6290..6337
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6666..7254
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atggactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
      661 gatgacaaga tgtcagccaa ggcaatttca gagcagaccg gcaaagaact cctgtacaag
      721 tacatctgca ccacctcggc catccagaac cggttcaagt acgcccgggt gactcccgac
      781 acagactggg cccatctgct gcaggaccac ccgtggctgc tcagccagag cttggttgtc
      841 aagccagacc agttaatcaa acgtcgagga aagcttggcc tcgtcggggt caatctctct
      901 ctggatggag tcaaatcctg gctaaaacct cgcctgggac atgaagccac tgtcggcaag
      961 gccaaaggct tcctcaagaa ctttctcatt gaacccttcg tcccccacag tcaggcggag
     1021 gagttctacg tgtgcatcta tgctacccgg gaaggagact acgtcctgtt ccaccatgaa
     1081 gggggtgtgg atgtgggcga tgtggatgcc aaagcccaga agctgcttgt gggtgtggac
     1141 gaaaagctga ataccgagga cattaagaga cacctgttgg tccatgcacc tgaggacaag
     1201 aaagaagtcc tggccagctt catctctggt ctattcaatt tctacgagga tctgtacttc
     1261 acctaccttg agatcaaccc ccttgtggtg accaaagatg gtgtctacat ccttgacttg
     1321 gcggccaagg tggatgccac agctgactac atctgtaaag tcaagtgggg tgatatagag
     1381 ttccctcccc cctttgggcg tgaggcgtac cccgaggaag cctacattgc agacctggat
     1441 gccaaaagtg gagcaagctt gaagctgacc ttgctgaacc ccaaggggcg gatctggacc
     1501 atggttgctg ggggtggcgc ctccgtcgtg tacagtgaca ccatctgtga tcttggaggt
     1561 gtcaatgaac tggcgaatta cggggaatac tcgggtgccc ccagtgaaca acagacctat
     1621 gactatgcca agaccatcct ctcacttatg actcgagaga agcacccaga aggcaagatc
     1681 ctcatcattg gaggcagcat tgcaaacttc accaatgtgg ccgccacctt caagggcatt
     1741 gtgagagcga ttcgagatta ccagggtccc ctgaaggagc atgaggtcac catctttgtc
     1801 cgaagaggtg gccccaacta tcaagaggga ttacgagtga tgggagaagt tgggaagacc
     1861 actgggatcc ccatccatgt ctttggcaca gaaactcaca tgacggccat tgtgggcatg
     1921 gccctgggcc accggcccat tcccaaccag ccacccacag cagctcacac tgccaacttc
     1981 ctccttaatg ccagcgggag cacatcgact ccagcaccca gtaggacagc atctttttct
     2041 gagtcccgag ctgatgaagt ggcacctgca aagaaagcca agccagctat gccccaagat
     2101 tcagtcccaa gtccaagatc cctgcaagga aagagtgcca ccctcttcag ccgacatacc
     2161 aaggccatcg tgtggggcat gcagacccgg gctgtgcagg gcatgctgga ctttgactac
     2221 gtgtgttccc gagacgagcc ctcagtggct gccatggtct accctttcac tggggatcac
     2281 aagcagaagt tttactgggg acacaaggaa atcctgatcc ctgtcttcaa gaacatggct
     2341 gacgccatga agaagcaccc ggaggtagac gtgctgatca actttgcgtc tctgcggtcc
     2401 gcttacgaca gcaccatgga gaccatgaac tatgcccaga tccgcaccat agccatcata
     2461 gcagaaggta tccctgaggc cctcacacgg aagctcatca agaaggccga ccagaaggga
     2521 gtgaccatca ttgggccagc tacggttggg ggcattaagc ctggatgctt taagatcggg
     2581 aatactggtg gaatgctgga caacatcctg gcctccaaac tgtaccgccc aggcagcgtg
     2641 gcctacgtct cacgttcagg aggcatgtct aatgaactca ataacatcat ctctcggacc
     2701 acagatggtg tctatgaggg cgtggccatc ggcggggaca ggtaccctgg gtccacattc
     2761 atggatcacg tgctgcgcta ccaggacact ccaggagtca agatgatcgt agttcttggg
     2821 gagatagggg gcacagagga atataagatc tgccggggca tcaaggaggg ccgcctcacc
     2881 aagccagtgg tctgctggtg tatcgggacc tgtgccacca tgttctcctc cgaggtccag
     2941 tttggccatg ctggagcttg tgccaaccag gcttctgaaa ctgcagtagc caagaaccag
     3001 gccttgaagg aagcaggagt gtttgtgccc cgaagcttcg atgagcttgg agaaatcatt
     3061 cagtctgtgt atgaagatct ggtggccaaa ggagccattg tacctgccca ggaagtgcca
     3121 cctccaacag tgcccatgga ctactcttgg gccagagagc tgggtttgat ccgaaaacct
     3181 gcctcattca tgaccagcat ctgtgatgag cgagggcagg agctcattta tgcgggcatg
     3241 cccatcaccg aggtcttcaa ggaggagatg ggcatcggtg gtgtcctcgg cctcctctgg
     3301 ttccagagaa ggttgcccaa gtattcctgc cagttcattg agatgtgtct gatggtcaca
     3361 gctgatcacg ggccagctgt ctctggagcc cataacacca tcatctgtgc tcgggctggg
     3421 aaggacctgg tctccagcct cacctcaggg ctgctcacca ttggagaccg gtttgggggt
     3481 gccttggatg ccgcagcaaa gatgttcagt aaagcctttg acagcggcat cattcccatg
     3541 gagttcgtca acaagatgaa gaaggagggg aagctgatca tgggcatcgg ccatcgagta
     3601 aaatcgataa acaacccaga catgcgagtg cagatcctca aggacttcgt caaacagcac
     3661 ttccccgcca ccccgctgct cgactatgcc ctggaagtgg agaagattac cacctccaag
     3721 aagccaaatc ttatcctgaa tgtggacggc ttcatcggcg ttgcgtttgt ggacatgctc
     3781 aggaactgtg gctccttcac ccgggaggaa gctgatgaat atgttgacat tggagccctc
     3841 aatggcatct ttgtgctagg aaggagtatg ggcttcattg ggcactacct tgaccagaag
     3901 aggctgaagc aagggctgta tcgtcacccc tgggatgaca tttcctatgt tcttccagaa
     3961 cacatgagca tgggtggctc actgcctccc ctggagcgcc tgaccctgta gggtaccgcg
     4021 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     4081 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4141 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4201 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4261 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     4321 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4381 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4441 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4501 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4561 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4621 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4681 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4741 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4801 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4861 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     4921 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4981 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5041 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5101 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5161 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5221 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5281 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5341 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5401 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     5461 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5521 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5581 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5641 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5701 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5761 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     5821 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5881 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5941 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6001 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6061 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6121 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6181 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     6241 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     6301 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     6361 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6421 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     6481 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     6541 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6601 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6661 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6721 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6781 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6841 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6901 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6961 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7021 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7081 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7141 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7201 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7261 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7321 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
