LOCUS       V038227                10485 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038227
VERSION     V038227
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1393..7140
                     /label="Atg2a(NM_001109545)"
                     /note="Atg2a(NM_001109545)"
                     /gene="Atg2a"
     polyA_signal    7273..7394
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(7401..7856)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7883..7987
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        7989..8346
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8381..9175
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9407..9454
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      9783..10371
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagg gtggcggtgg ctcgggcggt ggtgggtcgg gtggcggcgg atctatcgat
     1381 gaattggaat tcatgtcacg atggctgtgg ccatggtcga actgtgtgaa agagcgtgtc
     1441 tgccgctacc tgctacagca ctacttgggt cacttctttc aggagcacct cagcctggac
     1501 cagctcagcc tggatctgta caagggcagc gttgtcctgc gggatattca cctggaaacc
     1561 tggtctgtga acgaggtttt gaggtcaatg gagtcaccac tggaactggt ggaaggcttc
     1621 gtgagctcca tcgaggtggc tgtcccctgg gctgcacttc tcactgacca ctgcactgtg
     1681 tgtgtgtcag gccttcagct tatcctgcaa ccccgccagg gttcagggcc agaggctgca
     1741 gactctcaga cttgggcctc atgcatgacc acaagcctac agctggctca agagtgcctt
     1801 cgggaagggt tgccagaacc ctcagaacca ccacagcccc tagaggggct ggaaatgttt
     1861 gcacagacca ttgagactgt gctccggagg atcaaggtga ccttcctgaa cactgttgtg
     1921 agggtggagc attcactggg tgatgaggaa cggagtgtag ctgtggaggt ccgagtgcag
     1981 agactggaat actgtgacga agcagtgcgg gacccaagcc aggcaccacc tgtggacgtg
     2041 caccagccac ctgccttctt gcacaagctg ctgcagctga ctggggtctg cctgtacttt
     2101 gaagagctgc cctcccaggt ggacccgcca aagccacctt tgcaaatcgg cagctgcaca
     2161 gggtacatgg agctcatggt gaagctgaaa cagaatgagg ccttcccggg gcccaagttg
     2221 gaggtatcag gacagctggg atccctgcat ctgctcttga ccccgcgtca gctccagcag
     2281 ctccaaaaac tgctcagtgc catcaacctt gcagatccag gaggcctggc tgacaagctg
     2341 aacaagagcc gcccactagg tgctgaagac ttgtggctca ttgagcaaga tcttaaccag
     2401 cagctgcagg cgggtgcagt ggctgagtcc ctcagcctgc accccatcac cagccccctc
     2461 aatttggaca gtgcagacct attcttctcc atggctggcc tcacaagcag tgtgacctcc
     2521 ggtgtctctg agctctcagt gtatagtgta gacctgggct cctcggttca cagcaacatg
     2581 gccctccatc gaccctcaac cccaacccac tcaggtggca agacggctcc gactcccctt
     2641 ctggatacca tgcgccctga ctcactggtg aagatgacct tgggaggttt gagcctgacc
     2701 ttgcttcaga cagcttcccc atcttcagga tcacctgacc tcaccaccca cttttttgct
     2761 gagttcgatg ctgccaaaga tgggccgttc gggtcccgag acttctacca cctccggcca
     2821 cgcttccaga gggcctgccc ttgtagccat gtccgattaa caggcacagc cgtgcagctg
     2881 tcctgggagc tgcgtactgg cggccacagc cggaggacaa gcagcacaga agtgcacttc
     2941 ggacagctgg aggtgctgga gtgcctgtgg cccagggctg cctcagagcc agagtacaca
     3001 gagatcctga gcttccccag ttactctggc tccgaagcct cagctcggcc ctgcgcccat
     3061 ctgcgccaca cacaaacctt tcgccgggtg ctcaagagcc ggtcccggcg ctccactgcc
     3121 tgccattgtc actcagaact ctccctggac ctggctgatt tccaagctga cgtggagctg
     3181 gggtcgctgg accgccttgc tgtcttgctc cgtcaaatca ccacaccctc tgagccaccc
     3241 gctggcctac taacagagcc cccacaggcc actgagcagc agacagtgtt tcgtctctca
     3301 gcaccccggg ccaccctgag gctacgcttc cccatcccag acttacggcc tgaacgggac
     3361 ccctgggctg gccaggctgt acggactgag cagctgcgac tggagctgag tgagcctcag
     3421 ttccgctcag agctcaacag tgggcctggc cccccagctc ctacccgctt ggaacttact
     3481 tgcttagacc tacaaggaat ctacgaagat ggagagaagc cacctgtccc ctgcctgcgg
     3541 gtctctaagg ctctggatcc caggagcact gaggccaagt acttcctacc tcaggtagtg
     3601 gtgaccctga acccccagtc cagtggcaca cagtgggaga cagcctctga gaaaggccgg
     3661 gatctagaac tgtctaccga gagtccatgt gagctccagc agcctgagcc ctcacctttc
     3721 tcctccaagc ggactatgta tgaaactgag gagatggtga tccctggaga ccctgaggag
     3781 atgagggcat tccagaaccg gacgctggcg ctgtcccgct gtactctgga tgtgatcatg
     3841 cccagtgccc acatcttcct gcccagcaag gaggtctacg aaagcatcta caacaggatc
     3901 aacaatgacc tgctcatgtg ggagcctgca gacctacttc ccacatccac cactgctgct
     3961 cggccgcccg gctcttctgg gttcaagatg tgcaagtcag ccttcaagct agactcggat
     4021 tcggatgaag aagacaccca gttcttctcg atggcatcag gtgcccccca gactcctgcc
     4081 ccggaacctt cccatcgtca gtctcagagt accttctcca cactggtgac agtgctgaag
     4141 ggtcggatca ctgcgctctg tgaggctaag gatgagacag ggaagcggct ggatgccact
     4201 cacggggagc tggtactaga tgtggagcaa ggcaccatct tcagcgtagc ccagtaccgt
     4261 ggccagccgg ggctcggcta cttctgcttg gaagctgaaa aggcaacact ctaccatcga
     4321 gcggccattg aggactacct gctgcccact cacttggaag tacccagctt tgctccccca
     4381 gctcagctgg ctccaaccat ctacccatca gaggaagggg tgactgagcg gggaacattg
     4441 ggccgcaaga gccagggtcc ccccatgctg tctgcagctg tccgcatcca cctggacccc
     4501 cacaagaatg tcaaggaatt tttggtgaca atacggctgc acaaagccac tctacgccac
     4561 tacatggccc ctcctgagca gagctggcac tcccagctat tggacttctt ggatgtactg
     4621 gatgaccctg tgctgggtta cctgccccca acagtcatca ctattctgca cacacacctc
     4681 ttctcctgtg ctgtggatta caggcccctc tacctccctg tgcgtgtcct tatcactgct
     4741 gagaccttca ctctctccag caacattgtc atggacacgt cgaccttcct gctcaggttc
     4801 atcctcgatg actccgctct gtacctgtct gacaagtgtg aggtggagaa cctggacctg
     4861 cggcgagatt atgtctgcgt cctggacatt gacctcctgg agcttgtaat caaaacctgg
     4921 aagggcagca ccgagggcag actgagccag ccgctgtttg agctgcgctg ctccaacaat
     4981 gtggtgcatg tacacagctg tgctgactcc tgcgccctgc tggtcaacct gctccagtat
     5041 gtaactagct ccggtgacct gcatcccccg ccccagcccc ccagccctac ggagatcgcc
     5101 ggccagaagc tttcagagag cccggcctcc ctgccttcct gcctaccagt ggagacagcc
     5161 ctcatcaacc agcgagacct gactgatgcc cttctggaca ctgaacggcg tagcctacgg
     5221 gagctggccc agtcatcagg tggcccactt cctcaggcct ctcccgtctc cgtctatctg
     5281 ttcccaggtg aacggagtgg ggcccaggcc cccttgcctc ctcctggggg cccttctcat
     5341 accttggggt cctgttctga ggcaaaggaa catgacaaag aagaggaagg ggatggagac
     5401 accctggaca gtgacgagtt ctgcatcctt gacgcccctg gcctaggcat tgcgccccga
     5461 gacggtgagc ccatcgtgac tcagctgcat ccaggcccca tcgttgtgca tgatgggcac
     5521 ttctcccagc ccctgggaag cactgacctg ctgcgggcgc ctgcccactt cccagtgccc
     5581 agcagccgtg tggtactacg ggaggtctcc ttcatctggc acctttatgg gggtagagac
     5641 tttggccttc accccactta cagagcaaga gtaggcctcg caggccctag ggtctcacct
     5701 tcccgttcct ctggtcccaa ccggccacag aactcctggc gtacccaagg tggcattgga
     5761 cggcaacacc aggtcctcat ggagatccag ctcagcaaag tgagcttcca acatgaagta
     5821 tacccagaag aatcagccat agccgggggc ctggaccagg aactggatga gcgaccactg
     5881 tcccgccagg tgctcattgt tcaggagcta gaaatccgag accggctggc cacctccaag
     5941 atcaacaagt tccttcattt gcacacgagc gagcggctgc cacggcgcac acactccaac
     6001 atgcttacca tcaaggcact gcacgtggct cccaccggca gtgtaggagg gccggagtgc
     6061 tgtcttcgag tctccatgat gcctctgagg ctcaatgtgg accaggatgc ccttttcttc
     6121 ctcaaggact ttttcaccag cctagcagcc agcatcaacc ccatggtccc aggggacact
     6181 tttgaggctc accgagagac ccacagccgg cccagcagcc cccaggaagg gcagtctgag
     6241 ggcacagaga cagccagcag cccccaggaa gctccaggca gtagccacag ctcctctgac
     6301 caacagccca tctacttcag ggagttccgc ttcacatctg aggtgcccat ctgcctggat
     6361 taccatggca aacatgtcac tgtggaccag gtgggcactt tcatgggact cctaattggc
     6421 ctggcccagc tcaactgctc cgagctgaag ctcaagaggc tttgctgccg gcatgggctc
     6481 ctaggtgtgg acaaagtgct cggctatgcc ctcaatgaat ggctgcagga catccgaaag
     6541 aaccagctgc ctggcctgct gggaggcgtg ggtcccatgc attcggttgt acaactcttc
     6601 caggggttcc gggacctgct gtggctaccc atcgagcagt acaggaaaga tgggcgcctc
     6661 attcgggggc tgcagcgtgg cgctgcctcc tttgggtcat ccacagcctc tgctgctctg
     6721 gaactgagca acaggctggt ccaggccatc caggctacag ctgagacagt atatgacatc
     6781 ctgtcccctg catctcccat ctcccgatcc ctacaagaca aacgttcttc acggaaacta
     6841 cgcagaggtc aacaacctgc tgacctccgg gaaggcatgg ctaaggcata tgacgctgtt
     6901 cgagagggta tcctggatac agctcaaacc atctgtgatg tggcatcaag gggccatgag
     6961 cagaagggac tgacaggtgc cgtggggggt gtgatccgac agctcccccc aactgtggta
     7021 aaaccaataa ttgtagcaac agaggcaaca tccaacgtgc tagggggcat gcgtaaccag
     7081 atcctgccag atgcacacaa ggaccatgct ctcaagtggc gattggagga gacccaggac
     7141 tgaggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     7201 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     7261 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7321 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7381 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     7441 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     7501 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     7561 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     7621 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     7681 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     7741 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     7801 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     7861 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7921 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7981 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     8041 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8101 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8161 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8221 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8281 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8341 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     8401 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     8461 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     8521 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     8581 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     8641 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     8701 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     8761 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     8821 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     8881 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     8941 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     9001 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     9061 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     9121 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     9181 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     9241 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     9301 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     9361 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     9421 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     9481 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     9541 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     9601 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     9661 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9721 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9781 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9841 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9901 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     9961 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10021 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10081 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10141 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10201 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10261 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10321 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10381 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    10441 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
