LOCUS V024720 5406 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024720 VERSION V024720 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1320 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 1378..2061 /label="CD9(NM_001769)" /note="CD9(NM_001769)" /gene="CD9" polyA_signal 2194..2315 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2322..2777) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2804..2908 /gene="bla" /label="AmpR promoter" promoter 2910..3267 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3302..4096 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4328..4375 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4704..5292 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgagc tggaggtgga ggtagtggag gaggcggaag caagcttatg 1381 ccggtcaaag gaggcaccaa gtgcatcaaa tacctgctgt tcggatttaa cttcatcttc 1441 tggcttgccg ggattgctgt ccttgccatt ggactatggc tccgattcga ctctcagacc 1501 aagagcatct tcgagcaaga aactaataat aataattcca gcttctacac aggagtctat 1561 attctgatcg gagccggcgc cctcatgatg ctggtgggct tcctgggctg ctgcggggct 1621 gtgcaggagt cccagtgcat gctgggactg ttcttcggct tcctcttggt gatattcgcc 1681 attgaaatag ctgcggccat ctggggatat tcccacaagg atgaggtgat taaggaagtc 1741 caggagtttt acaaggacac ctacaacaag ctgaaaacca aggatgagcc ccagcgggaa 1801 acgctgaaag ccatccacta tgcgttgaac tgctgtggtt tggctggggg cgtggaacag 1861 tttatctcag acatctgccc caagaaggac gtactcgaaa ccttcaccgt gaagtcctgt 1921 cctgatgcca tcaaagaggt cttcgacaat aaattccaca tcatcggcgc agtgggcatc 1981 ggcattgccg tggtcatgat atttggcatg atcttcagta tgatcttgtg ctgtgctatc 2041 cgcaggaacc gcgagatggt ctagggatcc accggatcta gataactgat cataatcagc 2101 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 2161 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 2221 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 2281 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat 2341 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 2401 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 2461 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 2521 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 2581 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 2641 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 2701 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 2761 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 2821 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 2881 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg 2941 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 3001 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 3061 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 3121 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 3181 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 3241 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg 3301 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 3361 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 3421 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 3481 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 3541 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 3601 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 3661 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 3721 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 3781 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 3841 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 3901 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 3961 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 4021 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 4081 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 4141 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 4201 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 4261 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 4321 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 4381 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 4441 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 4501 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata 4561 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 4621 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 4681 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4741 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 4801 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 4861 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 4921 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 4981 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 5041 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 5101 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 5161 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 5221 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 5281 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 5341 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 5401 atgcat //