LOCUS       V046788                 8267 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046788
VERSION     V046788
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1375..4893
                     /label="CTR9(NM_014633)"
                     /note="CTR9(NM_014633)"
                     /gene="CTR9"
     polyA_signal    5055..5176
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5183..5638)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5665..5769
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5771..6128
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6163..6957
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7189..7236
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7565..8153
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt actcagatct cgagggtgga ggcggatcag gaggcggtgg atctatgtcg
     1381 cggggctcca tcgagattcc cctccgggac actgacgagg tcattgaact tgacttcgat
     1441 cagttaccgg agggagatga agttatcagt attctgaaac aggaacacac acaactgcac
     1501 atatggattg ctttggcgct ggaatactac aagcaaggaa aaacagaaga gtttgtaaaa
     1561 ttgttggaag cagcacgtat agatggcaat ttggactata gagaccatga aaaagaccag
     1621 atgacttgct tggatacatt ggcagcgtat tatgtacaac aggctcggaa agaaaagaat
     1681 aaggacaata aaaaggatct tattacacag gccaccttgt tgtatacaat ggccgataaa
     1741 attattatgt atgatcagaa ccatttgttg ggaagagcct gcttctgcct acttgagggt
     1801 gacaaaatgg atcaagctga tgcacagttt cattttgtac tcaatcagtc tccaaataat
     1861 attccagccc ttcttggtaa agcttgcatt tccttcaaca agaaggatta cagaggagct
     1921 cttgcttact ataagaaagc attgcgtact aacccaggat gtccagcgga agttcgttta
     1981 ggaatgggtc attgctttgt gaaacttaac aaactggaaa aagctcgtct ggcattcagc
     2041 agagccctgg aactcaattc caaatgcgtg ggagcattgg ttggactggc tgttctagaa
     2101 ctcaacaata aagaggctga ttccattaaa aatggtgtcc agcttctttc cagagcctat
     2161 actattgatc ctagcaaccc tatggtattg aaccatttgg caaatcactt tttcttcaaa
     2221 aaggattata gtaaagtcca gcatctggcc ctccatgcat tccataatac agaagtggaa
     2281 gctatgcaag cagagagctg ctatcagcta gctagatcat tccatgttca ggaagattat
     2341 gaccaagctt ttcagtacta ttatcaagcc acacagtttg cctcatcctc ttttgtgctc
     2401 ccattttttg gtttgggaca aatgtatatt tatcgaggtg acaaagaaaa tgcatctcag
     2461 tgctttgaga aggttttgaa agcttatcct aataattacg aaactatgaa aattctcggc
     2521 tctctctatg ctgcctcaga agatcaagaa aaacgagata ttgccaaggg ccatttgaag
     2581 aaggtcacag aacagtatcc cgatgatgtt gaagcttgga ttgaattggc acaaatctta
     2641 gaacagactg atatacaggg tgccctttca gcctatggaa cagcaacacg aatccttcag
     2701 gagaaagtgc aggccgatgt tcctccagag attctcaata atgtgggtgc cctccatttt
     2761 agacttggaa acctagggga ggctaagaaa tattttttgg cgtcattgga ccgtgcaaaa
     2821 gcagaagcgg aacacgatga gcattactat aacgccattt ccgttaccac gtcatataat
     2881 ctcgccaggc tatatgaggc gatgtgtgaa ttccatgaag cagaaaaact gtataaaaac
     2941 atcttacgcg aacatcctaa ttatgttgac tgctatttgc gcctaggagc catggctaga
     3001 gataagggaa acttttatga ggcttcagat tggtttaagg aagctcttca gattaatcag
     3061 gatcatccag atgcttggtc tttgattggc aatcttcatt tggcaaaaca agaatggggt
     3121 cctgggcaga agaagtttga gaggatatta aaacagccat ccacacagag tgatacctat
     3181 tctatgctag cccttggcaa cgtgtggctc caaactttac atcagcccac ccgagatcga
     3241 gaaaaggaaa agcgtcatca agatcgtgct ctggccatct acaaacaagt actcagaaat
     3301 gatgcaaaga atctgtatgc tgccaatggc ataggagctg ttttggccca caaaggatat
     3361 tttcgtgaag ctcgtgatgt atttgcccaa gtaagagaag caacagcaga tattagtgat
     3421 gtgtggctga acttagcaca catctatgtg gagcaaaagc agtacatcag cgccgttcag
     3481 atgtatgaaa actgcctccg aaagttctat aagcaccaaa acactgaagt tgtactctat
     3541 ttggcccggg ccctcttcaa gtgtggcaag ttacaggaat gcaaacagac tttgctgaag
     3601 gctagacatg tggcacccag tgatacagtt cttatgttta atgtggcctt ggtcctgcaa
     3661 agattagcta cctctgtcct gaaagatgaa aaaagtaatc tgaaggaagt acttaatgct
     3721 gtgaaagaac tggagcttgc acatagatac ttcagttatt tgagtaaagt gggagataaa
     3781 atgagatttg atttggccct tgctgctaca gaagccaggc agtgttctga cttactgagc
     3841 caggcccagt accatgtggc ccgggcacgc aaacaagatg aagaagagcg ggagctgcgg
     3901 gccaagcaag agcaagaaaa ggagctgtta aggcagaaac ttcttaaaga acaggaagag
     3961 aaacgtctca gagaaaagga agagcaaaag aaacttttgg aacagcgggc ccagtatgtg
     4021 gagaagacca aaaatattct tatgtttact ggtgagactg aagcaacaaa agagaagaaa
     4081 agaggtggtg gtggtggacg gcgttctaag aagggaggag agtttgatga atttgtcaat
     4141 gatgacactg atgatgacct acctatatcc aaaaagaaga agagaagaaa gggtagtggc
     4201 agtgaacaag aaggtgaaga tgaggagggt ggtgagagaa agaagaaaaa gaggagaaga
     4261 catccaaagg gagaagaagg atctgatgat gatgaaacag aaaatggccc caaaccaaaa
     4321 aaacgacgtc caccaaaagc agagaagaaa aaggctccca agccagaacg tctgcctcca
     4381 tcaatgaagg gaaaaataaa atccaaagcc ataatttcat caagtgatga ctcttcggat
     4441 gaggataaac ttaaaattgc tgatgaagga catcccagga acagcaacag caacagtgac
     4501 tcagacgagg acgaacaacg aaagaaatgt gcctcatcag agagtgattc cgatgagaac
     4561 cagaacaagt ctggcagcga ggccggcagt ccccggaggc cacgaagaca gcggtcagat
     4621 caggactcag acagtgacca gccatccaga aagagaaggc cctccggttc tgagcagtct
     4681 gacaatgaat ctgtgcagtc agggagaagc cactcaggag tttctgagaa cgactctcgc
     4741 ccagcttctc caagtgccga atcagatcac gaatcggaga gaggatctga taatgagggt
     4801 tctggccaag gctctggaaa tgaatcggaa ccagagggat ccaacaatga ggcctcagat
     4861 agaggctcag aacatgggtc agatgatagt gactgaattc tgcagtcgac ggtaccgcgg
     4921 gcccgggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     4981 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     5041 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5101 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5161 tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     5221 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     5281 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     5341 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     5401 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     5461 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     5521 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     5581 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     5641 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5701 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5761 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     5821 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5881 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5941 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6001 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6061 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6121 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     6181 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6241 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6301 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     6361 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6421 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6481 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6541 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6601 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6661 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     6721 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6781 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6841 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6901 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6961 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     7021 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7081 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
     7141 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     7201 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     7261 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     7321 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     7381 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
     7441 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7501 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7561 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7621 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7681 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7741 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7801 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7861 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7921 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7981 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8041 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8101 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8161 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8221 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
