LOCUS       V046594                 7040 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046594
VERSION     V046594
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1375..3666
                     /label="CUL2(NM_001198778)"
                     /note="CUL2(NM_001198778)"
                     /gene="CUL2"
     polyA_signal    3828..3949
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3956..4411)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4438..4542
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4544..4901
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4936..5730
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5962..6009
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6338..6926
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt actcagatct cgagggtgga ggcggatcag gaggcggtgg atctatgtac
     1381 agagtaacat ggtcaacttt ttggcttaga tttcaacact acacttgcac aatgtctttg
     1441 aaaccaagag tagtagattt tgatgaaaca tggaacaaac ttttgacgac aataaaagcc
     1501 gtggtcatgt tggaatacgt cgaaagagca acatggaatg accgtttctc agatatctat
     1561 gctttatgtg tggcctatcc tgaacccctt ggagaaagac tttatacaga aactaagatt
     1621 tttttggaaa atcatgttcg gcatttgcat aagagagttt tggagtcaga agaacaagta
     1681 cttgttatgt atcataggta ctgggaagaa tacagcaagg gtgcagacta tatggactgc
     1741 ttatataggt atctcaacac ccagtttatt aaaaagaata aattaacaga agcggacctt
     1801 cagtatggct atggtggtgt agatatgaat gaaccactta tggaaatagg agagctagca
     1861 ttggatatgt ggaggaaatt gatggttgaa ccacttcagg ccatccttat ccgaatgctg
     1921 ctccgagaaa tcaaaaatga tcgtggtgga gaagacccaa accagaaagt aatccatggg
     1981 gttattaact cctttgttca tgttgaacag tataagaaaa aattcccctt aaagttttat
     2041 caggaaattt ttgagtctcc ctttctgact gaaacaggag agtattacaa acaagaagct
     2101 tcaaatttat tacaagaatc aaactgctca cagtatatgg aaaaggttct aggtagatta
     2161 aaagatgaag aaattcgatg tcgaaaatac ctacatccaa gttcatatac taaggtgatt
     2221 catgaatgtc aacaacgaat ggtagcagac cacttacagt ttttacatgc agaatgtcat
     2281 aatataattc gacaagagaa aaaaaatgac atggcaaata tgtacgtctt actccgtgct
     2341 gtgtccactg gtttacctca tatgattcag gagctgcaaa accacatcca tgatgagggc
     2401 cttcgagcaa ccagcaacct tactcaggaa aacatgccaa cactatttgt ggagtcagtt
     2461 ttggaagtgc atggtaaatt tgttcagctt atcaacactg ttttgaatgg tgatcagcat
     2521 tttatgagtg cgttggataa ggcccttacg tcagttgtaa attacagaga acctaagtct
     2581 gtttgcaaag cacctgaact gcttgctaag tactgtgaca acttactgaa gaagtcagcg
     2641 aaagggatga cagagaatga agtggaagac aggctcacga gcttcatcac agtgttcaaa
     2701 tacattgatg acaaggacgt ctttcaaaag ttctacgcaa gaatgctggc aaaacgttta
     2761 attcatgggt tatccatgtc tatggactct gaagaagcca tgatcaacaa attaaagcaa
     2821 gcctgtggtt atgagtttac cagcaagcta catcggatgt atacagatat gagtgtcagc
     2881 gctgatctca acaataagtt caacaatttt atcaaaaacc aagacacagt aatagatttg
     2941 ggaattagtt ttcaaatata tgttctacag gctggtgcgt ggcctcttac tcaggctcct
     3001 tcatctacgt ttgcaattcc ccaggaatta gaaaaaagtg tacagatgtt tgaattattt
     3061 tatagccaac atttcagtgg aaggaaactt acatggttac attatctgtg tacaggtgaa
     3121 gttaaaatga actatttggg caaaccatat gtagccatgg ttacaacata ccaaatggca
     3181 gttcttcttg cctttaacaa cagtgaaact gtcagttata aagagcttca ggacagcact
     3241 cagatgaatg aaaaggaact gacaaaaaca atcaaatcat tacttgatgt gaaaatgatt
     3301 aaccatgatt cagaaaagga agatattgat gcagaatctt cgttttcatt aaatatgaac
     3361 tttagcagta aaagaacaaa atttaaaatt actacatcaa tgcagaaaga cacaccacaa
     3421 gaaatggagc agactagaag tgcagttgat gaggaccgga aaatgtatct ccaagctgct
     3481 atagttcgta tcatgaaagc acgaaaagtg cttcggcaca atgcccttat tcaagaggtg
     3541 attagccagt caagagctag gtttaatccc agtatcagca tgattaagaa gtgtattgaa
     3601 gttctgatag acaaacaata catagaacgc agccaggcgt cggcagatga atacagctac
     3661 gtcgcgtgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac
     3721 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     3781 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     3841 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3901 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt
     3961 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     4021 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     4081 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     4141 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     4201 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     4261 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     4321 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     4381 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     4441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     4561 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4621 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4681 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4741 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4801 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4861 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     4921 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4981 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5041 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5101 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5161 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5221 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5281 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5341 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5401 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5461 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5521 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5581 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5641 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5701 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     5761 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     5821 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5881 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     5941 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     6001 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     6061 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     6121 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     6181 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6241 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6481 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6961 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7021 accgtattac cgccatgcat
//
