LOCUS V019005 5014 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019005 VERSION V019005 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 624..938 /label="Cycs(NM_007808)" /note="Cycs(NM_007808)" /gene="Cycs" CDS 960..1679 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 1802..1923 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(1930..2385) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2412..2516 /gene="bla" /label="AmpR promoter" promoter 2518..2875 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2910..3704 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3936..3983 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4312..4900 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgacct aatatgggtg atgttgaaaa aggcaagaag atttttgttc 661 agaagtgtgc ccagtgccac actgtggaaa agggaggcaa gcataagact ggaccaaatc 721 tccacggtct gttcgggcgg aagacaggcc aggctgctgg attctcttac acagatgcca 781 acaagaacaa aggcatcacc tggggagagg ataccctgat ggagtatttg gagaatccca 841 aaaagtacat ccctggaaca aaaatgatct tcgctggaat taagaagaag ggagaaaggg 901 cagacctaat agcttatctt aaaaaggcta ctaatgagaa agatccaccg gtcgccacca 961 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 1021 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 1081 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 1141 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 1201 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 1261 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 1321 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 1381 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 1441 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 1501 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 1561 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 1621 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa 1681 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt 1741 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 1801 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 1861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 1921 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa 1981 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat 2041 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg 2101 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac 2161 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta 2221 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag 2281 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg 2341 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc 2401 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 2461 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg 2521 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 2581 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 2641 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 2701 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 2761 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 2821 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg 2881 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 2941 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 3001 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 3061 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg 3121 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 3181 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 3241 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 3301 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 3361 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 3421 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 3481 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 3541 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 3601 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 3661 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 3721 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 3781 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 3841 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg 3901 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac 3961 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt 4021 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc 4081 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc 4141 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt 4201 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 4261 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 4321 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 4381 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 4441 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 4501 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 4561 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 4621 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 4681 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 4741 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 4801 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 4861 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 4921 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 4981 tgattctgtg gataaccgta ttaccgccat gcat //