LOCUS       V032784                 9264 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032784
VERSION     V032784
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..5919
                     /label="EPRS1(NM_004446)"
                     /note="EPRS1(NM_004446)"
                     /gene="EPRS1"
     polyA_signal    6052..6173
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(6180..6635)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6662..6766
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        6768..7125
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7160..7954
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8186..8233
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8562..9150
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatggcga cgctctctct gaccgtgaat tcaggagacc ctccgctagg agctttgctg
     1441 gcagtagaac acgtgaaaga cgatgtcagc atttccgttg aagaagggaa agagaatatt
     1501 cttcatgttt ctgaaaatgt gatattcaca gatgtgaatt ctatacttcg ctacttggct
     1561 agagttgcaa ctacagctgg gttatatggc tctaatctga tggaacatac tgagattgat
     1621 cactggttgg agttcagtgc tacaaaatta tcttcatgtg attcctttac ttctacaatt
     1681 aatgaactca atcattgcct gtctctgaga acatacttag ttggaaactc cttgagttta
     1741 gcagatttat gtgtttgggc caccctaaaa ggaaatgctg cctggcaaga acagttgaaa
     1801 cagaagaaag ctccagttca tgtaaaacgt tggtttggct ttcttgaagc ccagcaggcc
     1861 ttccagtcag taggtaccaa gtgggatgtt tcaacaacca aagctcgagt ggcacctgag
     1921 aaaaagcaag atgttgggaa atttgttgag cttccaggtg cggagatggg aaaggttacc
     1981 gtcagatttc ctccagaggc cagtggttac ttacacattg ggcatgcaaa agctgctctt
     2041 ctgaaccagc actaccaggt taactttaaa gggaaactga tcatgagatt tgatgacaca
     2101 aatcctgaaa aagaaaagga agattttgag aaggttatct tggaagatgt tgcaatgttg
     2161 catatcaaac cagatcaatt tacttatact tcggatcatt ttgaaactat aatgaagtat
     2221 gcagagaagc taattcaaga agggaaggct tatgtggatg atactcctgc tgaacagatg
     2281 aaagcagaac gtgagcagag gatagactct aaacatagaa aaaaccctat tgagaagaat
     2341 ctacaaatgt gggaagaaat gaaaaaaggg agccagtttg gtcagtcctg ttgtttgcga
     2401 gcaaaaattg acatgagtag taacaatgga tgcatgagag atccaaccct ttatcgctgc
     2461 aaaattcaac cacatccaag aactggaaat aaatacaatg tttatccaac atatgatttt
     2521 gcctgcccca tagttgacag catcgaaggt gttacacatg ccctgagaac aacagaatac
     2581 catgacagag atgagcagtt ttactggatt attgaagctt taggcataag aaaaccatat
     2641 atttgggaat atagtcggct aaatctcaac aacacagtgc tatccaaaag aaaactcaca
     2701 tggtttgtca atgaaggact agtagatgga tgggatgacc caagatttcc tacggttcgt
     2761 ggtgtactga gaagagggat gacagttgaa ggactgaaac agtttattgc tgctcagggc
     2821 tcctcacgtt cagtcgtgaa catggagtgg gacaaaatct gggcgtttaa caaaaaggtt
     2881 attgacccag tggctccacg atatgttgca ttactgaaga aagaagtgat cccagtgaat
     2941 gtacctgaag ctcaggagga gatgaaagaa gtagccaaac acccaaagaa tcctgaggtt
     3001 ggcttgaagc ctgtgtggta tagtcccaaa gttttcattg aaggtgctga tgcagagact
     3061 ttttcggagg gtgagatggt tacatttata aattggggca acctcaacat tacaaaaata
     3121 cacaaaaatg cagatggaaa aatcatatct cttgatgcaa agttgaattt ggaaaacaaa
     3181 gactacaaga aaaccactaa ggtcacttgg cttgcagaga ctacacatgc tcttcctatt
     3241 ccagtaatct gtgtcactta tgagcacttg atcacaaagc cagtgctagg aaaagacgag
     3301 gactttaagc agtatgtcaa caagaacagt aagcatgaag agctaatgct aggggatccc
     3361 tgccttaagg atttgaaaaa aggagatatt atacaactcc agagaagagg attcttcata
     3421 tgtgatcaac cttatgaacc tgttagccca tatagttgca aggaagcccc gtgtgttttg
     3481 atatacattc ctgatgggca cacaaaggaa atgccaacat cagggtcaaa ggaaaagacc
     3541 aaagtagaag ccacaaaaaa tgagacctct gctcctttta aggaaagacc aacaccttct
     3601 ctgaataata attgtactac atctgaggat tccttggtcc tttacaatag agtggctgtt
     3661 caaggagatg tggttcgtga attaaaagcc aagaaagcac caaaggaaga tgtagatgca
     3721 gctgtaaaac agcttttgtc tttgaaagct gaatataagg agaaaactgg ccaggaatat
     3781 aaacctggaa accctcctgc tgaaatagga cagaatattt cttctaattc ctcagcaagt
     3841 attctggaaa gtaaatctct gtatgatgaa gttgctgcac aaggggaggt ggttcgtaag
     3901 ctaaaagctg aaaaatcccc taaggctaaa ataaatgaag ctgtagaatg cttactgtcc
     3961 ctgaaggctc agtataaaga aaaaactggg aaggagtaca tacctggtca gcccccatta
     4021 tctcaaagtt cggattcaag cccaaccaga aattctgaac ctgctggttt agaaacacca
     4081 gaagcgaaag tactttttga caaagtagct tctcaagggg aagtagttcg gaaacttaaa
     4141 actgaaaaag cccctaagga tcaagtagat atagctgttc aagaactcct tcagctaaag
     4201 gcacagtaca agtctttgat aggagtagag tataagcctg tgtcggccac tggagctgag
     4261 gacaaagata agaagaagaa agaaaaagaa aataaatctg aaaagcagaa taagcctcag
     4321 aaacaaaatg atggccaaag gaaagaccct tctaaaaacc aaggaggtgg gctctcatca
     4381 agtggagcag gagaagggca ggggcctaag aaacagacca ggttgggtct tgaggcaaaa
     4441 aaagaagaaa atcttgctga ttggtattct caggtcatca caaagtcaga aatgattgaa
     4501 taccatgaca taagtggctg ttatattctt cgtccctggg cctatgccat ttgggaagcc
     4561 atcaaggact tttttgatgc tgagatcaag aaacttggtg ttgaaaactg ctacttcccc
     4621 atgtttgtgt ctcaaagtgc attagagaaa gagaagactc atgttgctga ctttgcccca
     4681 gaggttgctt gggttacaag atctggcaaa accgagctgg cagaaccaat tgccattcgt
     4741 cctactagtg aaacagtaat gtatcctgca tatgcaaaat gggtacagtc acacagagac
     4801 ctgcccatca agctcaatca gtggtgcaat gtggtgcgtt gggaattcaa gcatcctcag
     4861 cctttcctac gtactcgtga atttctttgg caggaagggc acagtgcttt tgctaccatg
     4921 gaagaggcag cggaagaggt cttgcagata cttgacttat atgctcaggt atatgaagaa
     4981 ctcctggcaa ttcctgttgt taaaggaaga aagacggaaa aggaaaaatt tgcaggagga
     5041 gactatacaa ctacaataga agcatttata tctgctagtg gaagagctat ccagggagga
     5101 acatcacatc atttagggca gaatttttcc aaaatgtttg aaatcgtttt tgaagatcca
     5161 aagataccag gagagaagca atttgcctat caaaactcct ggggcctgac aactcgaact
     5221 attggtgtta tgaccatggt tcatggggac aacatgggtt tagtattacc accccgtgta
     5281 gcatgtgttc aggtggtgat tattccttgt ggcattacca atgcactttc tgaagaagac
     5341 aaagaagcgc tgattgcaaa atgcaatgat tatcgaaggc gattactcag tgttaacatc
     5401 cgcgttagag ctgatttacg agataattat tctccaggtt ggaaattcaa tcactgggag
     5461 ctcaagggag ttcccattag acttgaagtt gggccacgtg atatgaagag ctgtcagttt
     5521 gtagccgtca gacgagatac tggagaaaag ctgacagttg ctgaaaatga ggcagagact
     5581 aaacttcaag ctattttgga agacatccag gtcacccttt tcacaagggc ttctgaagac
     5641 cttaagactc atatggttgt ggctaataca atggaagact ttcagaagat actagattct
     5701 ggaaagattg ttcagattcc attctgtggg gaaattgact gtgaggactg gatcaaaaag
     5761 accactgcca gggatcaaga tcttgaacct ggtgctccat ccatgggagc taaaagcctt
     5821 tgcatcccct tcaaaccact ctgtgaactg cagcctggag ccaaatgtgt ctgtggcaag
     5881 aaccctgcca agtactacac cttatttggt cgcagctact gaggatccac cggatctaga
     5941 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     6001 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     6061 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6121 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     6181 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     6241 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     6301 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     6361 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     6421 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     6481 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     6541 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     6601 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     6661 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6721 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     6781 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     6841 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6901 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6961 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7021 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7081 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     7141 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     7201 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     7261 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     7321 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     7381 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7441 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     7501 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7561 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7621 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7681 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     7741 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7801 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7861 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7921 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     7981 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     8041 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     8101 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     8161 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     8221 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     8281 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     8341 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     8401 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     8461 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8521 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8581 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8641 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8701 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8761 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8821 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8881 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8941 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9001 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9061 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9121 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9181 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9241 gataaccgta ttaccgccat gcat
//
