LOCUS       V023007                 7244 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023007
VERSION     V023007
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1342..3807
                     /label="FGFR1(NM_023110)"
                     /note="FGFR1(NM_023110)"
                     /gene="FGFR1"
     CDS             3814..3840
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3844..3870
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3874..3900
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4032..4153
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4160..4615)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4642..4746
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4748..5105
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5140..5934
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6166..6213
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6542..7130
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tatgtggagc tggaagtgcc tcctcttctg ggctgtgctg
     1381 gtcacagcca cactctgcac cgctaggccg tccccgacct tgcctgaaca agcccagccc
     1441 tggggagccc ctgtggaagt ggagtccttc ctggtccacc ccggtgacct gctgcagctt
     1501 cgctgtcggc tgcgggacga tgtgcagagc atcaactggc tgcgggacgg ggtgcagctg
     1561 gcggaaagca accgcacccg catcacaggg gaggaggtgg aggtgcagga ctccgtgccc
     1621 gcagactccg gcctctatgc ttgcgtaacc agcagcccct cgggcagtga caccacctac
     1681 ttctccgtca atgtttcaga tgctctcccc tcctcggagg atgatgatga tgatgatgac
     1741 tcctcttcag aggagaaaga aacagataac accaaaccaa accgtatgcc cgtagctcca
     1801 tattggacat ccccagaaaa gatggaaaag aaattgcatg cagtgccggc tgccaagaca
     1861 gtgaagttca aatgcccttc cagtgggacc ccaaacccca cactgcgctg gttgaaaaat
     1921 ggcaaagaat tcaaacctga ccacagaatt ggaggctaca aggtccgtta tgccacctgg
     1981 agcatcataa tggactctgt ggtgccctct gacaagggca actacacctg cattgtggag
     2041 aatgagtacg gcagcatcaa ccacacatac cagctggatg tcgtggagcg gtcccctcac
     2101 cggcccatcc tgcaagcagg gttgcccgcc aacaaaacag tggccctggg tagcaacgtg
     2161 gagttcatgt gtaaggtgta cagtgacccg cagccgcaca tccagtggct aaagcacatc
     2221 gaggtgaatg ggagcaagat tggcccagac aacctgcctt atgtccagat cttgaagact
     2281 gctggagtta ataccaccga caaagagatg gaggtgcttc acttaagaaa tgtctccttt
     2341 gaggacgcag gggagtatac gtgcttggcg ggtaactcta tcggactctc ccatcactct
     2401 gcatggttga ccgttctgga agccctggaa gagaggccgg cagtgatgac ctcgcccctg
     2461 tacctggaga tcatcatcta ttgcacaggg gccttcctca tctcctgcat ggtggggtcg
     2521 gtcatcgtct acaagatgaa gagtggtacc aagaagagtg acttccacag ccagatggct
     2581 gtgcacaagc tggccaagag catccctctg cgcagacagg taacagtgtc tgctgactcc
     2641 agtgcatcca tgaactctgg ggttcttctg gttcggccat cacggctctc ctccagtggg
     2701 actcccatgc tagcaggggt ctctgagtat gagcttcccg aagaccctcg ctgggagctg
     2761 cctcgggaca gactggtctt aggcaaaccc ctgggagagg gctgctttgg gcaggtggtg
     2821 ttggcagagg ctatcgggct ggacaaggac aaacccaacc gtgtgaccaa agtggctgtg
     2881 aagatgttga agtcggacgc aacagagaaa gacttgtcag acctgatctc agaaatggag
     2941 atgatgaaga tgatcgggaa gcataagaat atcatcaacc tgctgggggc ctgcacgcag
     3001 gatggtccct tgtatgtcat cgtggagtat gcctccaagg gcaacctgcg ggagtacctg
     3061 caggcccgga ggcccccagg gctggaatac tgctacaacc ccagccacaa cccagaggag
     3121 cagctctcct ccaaggacct ggtgtcctgc gcctaccagg tggcccgagg catggagtat
     3181 ctggcctcca agaagtgcat acaccgagac ctggcagcca ggaatgtcct ggtgacagag
     3241 gacaatgtga tgaagatagc agactttggc ctcgcacggg acattcacca catcgactac
     3301 tataaaaaga caaccaacgg ccgactgcct gtgaagtgga tggcacccga ggcattattt
     3361 gaccggatct acacccacca gagtgatgtg tggtctttcg gggtgctcct gtgggagatc
     3421 ttcactctgg gcggctcccc ataccccggt gtgcctgtgg aggaactttt caagctgctg
     3481 aaggagggtc accgcatgga caagcccagt aactgcacca acgagctgta catgatgatg
     3541 cgggactgct ggcatgcagt gccctcacag agacccacct tcaagcagct ggtggaagac
     3601 ctggaccgca tcgtggcctt gacctccaac caggagtacc tggacctgtc catgcccctg
     3661 gaccagtact cccccagctt tcccgacacc cggagctcta cgtgctcctc aggggaggat
     3721 tccgtcttct ctcatgagcc gctgcccgag gagccctgcc tgccccgaca cccagcccag
     3781 cttgccaatg gcggactcaa acgccgcaag ctttacccat acgacgtacc tgattatgca
     3841 acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca
     3901 taggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
     3961 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     4021 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4081 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4141 tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     4201 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     4261 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     4321 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     4381 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     4441 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     4501 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     4561 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     4621 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4681 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4741 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4801 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4861 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4921 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4981 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5041 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5101 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     5161 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5221 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5281 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     5341 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5401 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5461 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5521 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5581 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5641 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     5701 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5761 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5821 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5881 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5941 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     6001 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6061 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     6121 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     6181 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     6241 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     6301 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     6361 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     6421 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6661 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7201 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
