LOCUS       V024930                 6754 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024930
VERSION     V024930
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             624..2642
                     /label="FXR2(NM_004860)"
                     /note="FXR2(NM_004860)"
                     /gene="FXR2"
     CDS             2700..3419
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3542..3663
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3670..4125)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4152..4256
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4258..4615
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4650..5444
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5676..5723
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6052..6640
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatgggcg gcctggcctc tgggggggat gtggagccgg
      661 gactgcccgt cgaggtgcgc ggctccaacg gggccttcta caagggcttt gtgaaggatg
      721 tccatgaaga ctctgtcacc atcttctttg aaaacaactg gcagagtgag agacaaattc
      781 cttttgggga tgtccggcta ccacctccag ctgactataa taaggagatc acagaagggg
      841 atgaagtgga ggtttattct cgagccaatg aacaggaacc ttgtggctgg tggctggccc
      901 gggtgcggat gatgaaggga gatttctatg tcattgaata tgctgcctgt gatgccacct
      961 acaatgaaat tgttaccctg gagcgacttc ggccagttaa tcccaatccc cttgcaacca
     1021 aaggcagctt cttcaaggtt accatggctg tgcccgagga tctgagagaa gcctgctcca
     1081 atgaaaacgt ccataaagag ttcaagaaag ccctgggagc caactgcatc tttctcaaca
     1141 tcacaaacag tgagctcttc attctgtcaa ccacagaagc ccctgtgaag cgagcatctc
     1201 tgctgggtga tatgcatttc cgaagcctgc gcaccaaact gctacttatg tcccgcaatg
     1261 aagaagctac caagcaccta gagacaagca agcagttggc agcagccttc caagaggagt
     1321 tcacagtgcg agaggacctg atgggactgg caattgggac tcacggtgcc aacatccagc
     1381 aggcccgaaa agtacctggg gtgaccgcca ttgagttggg tgaagagacc tgcactttcc
     1441 gcatctatgg ggagactccc gaggcttgcc gacaggcccg aagctacctt gagttttctg
     1501 aggactcagt gcaagtgccc aggaacctgg ttggcaaagt gattggaaag aacgggaaag
     1561 tgatccagga gattgtggat aaatctggtg tggtgagggt tcgagtggaa ggtgataatg
     1621 acaagaagaa ccccagggag gagggaatgg ttcccttcat ttttgttggc acccgagaga
     1681 acatcagcaa tgcccaggct ttgctggagt atcacctctc ctacctgcag gaggtagagc
     1741 agcttcgctt ggagaggcta caaattgatg agcagcttcg gcagattggg ctgggctttc
     1801 gccctcctgg gagtgggcgg ggcagcggtg gcagcgacaa ggctggatat agcactgatg
     1861 agagctcctc ctcctccctc catgcgactc gaacctatgg gggcagctat gggggccgtg
     1921 gccgtggccg gaggacaggc ggtcctgcct atggccccag ctcagatgtg tctacagctt
     1981 cagagactga gtcagagaag agagaggagc ccaaccgagc tgggcctggc gacagggatc
     2041 ccccaacccg aggggaagaa agccggaggc ggccgactgg gggccggggt aggggacccc
     2101 cacctgcccc ccggcccact tcgagataca attcttcatc tattagctca gtgctgaagg
     2161 atccagacag taatccctac agcctattgg acacgtctga accagagccc ccggttgatt
     2221 cagaacctgg ggaacccccc ccagcaagtg ccaggcgccg ccgctcccgc cgccgccgca
     2281 ctgatgaaga caggaccgtc atggatggag gcctggaatc agatgggccc aacatgacag
     2341 agaatggcct ggaagatgaa tcaagacctc aacgtcgtaa tcgcagccgc cgccgccgta
     2401 accgtggtaa tcggactgat ggctctatca gtggagaccg ccagccagtg actgtggctg
     2461 actatatctc acgagcagag tctcagagcc gccagaggcc acccctggaa cgcactaaac
     2521 cctcagaaga ctctctttca ggacagaagg gtgactctgt cagcaagctt cctaagggcc
     2581 cctcggagaa tggggagctc tccgccccct tggagttggg tagtatggtg aatggggttt
     2641 caaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca
     2701 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2761 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2821 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2881 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     2941 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3001 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3061 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3121 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     3181 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3241 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3301 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3361 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa
     3421 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3481 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3541 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3601 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3661 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     3721 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     3781 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     3841 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     3901 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     3961 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     4021 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     4081 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     4141 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4201 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     4261 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4321 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4381 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4441 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4501 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4561 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     4621 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     4681 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4741 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4801 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     4861 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4921 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4981 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5041 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5101 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5161 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     5221 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5281 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5341 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5401 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5461 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5521 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5581 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     5641 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     5701 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     5761 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     5821 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     5881 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     5941 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6001 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6061 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6121 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6181 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6241 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6301 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6361 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6421 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6481 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6541 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6601 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6661 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6721 tgattctgtg gataaccgta ttaccgccat gcat
//
