LOCUS       V040377                 6174 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040377
VERSION     V040377
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             603..1319
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric optimized variant of ECFP (Kremers et
                     al., 2006)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNG
                     IKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="SCFP3A"
     CDS             1350..2798
                     /label="ICA1(NM_022307)"
                     /note="ICA1(NM_022307)"
                     /gene="ICA1"
     polyA_signal    2962..3083
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3090..3545)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3572..3676
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3678..4035
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4070..4864
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5096..5143
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5472..6060
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      601 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      721 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      781 ccctcgtgac caccctgagc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga
      841 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      901 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      961 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1021 acaagctgga gtacaactac ttcagcgaca acgtctatat caccgccgac aagcagaaga
     1081 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
     1141 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1201 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1261 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     1321 gtggaggcgg atcaggaggc ggtggatcta tgtcaggaca caaatgcagt tatccctggg
     1381 acttacagga tcgatatgct caagataagt cagttgtaaa taagatgcaa cagaaatatt
     1441 gggagacgaa gcaggccttt attaaagcca cagggaagaa ggaagatgaa catgttgttg
     1501 cctctgacgc ggacctggat gccaagctag agctgtttca ttcaattcag agaacctgtc
     1561 tggacttatc gaaagcaatt gtactctatc aaaagaggat atgtttcttg tctcaagaag
     1621 aaaacgaact gggaaaattt cttcgatccc aaggtttcca agataaaacc agagcaggaa
     1681 agatgatgca agcgacagga aaggccctct gcttttcttc ccagcaaagg ttggccttac
     1741 gaaatccttt gtgtcgattt caccaagaag tggagacttt tcggcatcgg gccatctcag
     1801 atacttggct gacggtgaac cgcatggaac agtgcaggac ggaatataga ggagcactat
     1861 tatggatgaa ggacgtgtct caggagcttg atccagacct ctacaagcaa atggagaagt
     1921 tcaggaaggt acaaacacaa gtgcgccttg caaaaaaaaa ctttgacaaa ttgaagatgg
     1981 atgtttgtca aaaagtggat cttcttggag cgagcagatg caatctcttg tctcacatgc
     2041 tagcaacata ccagaccact ctgcttcatt tttgggagaa aacttctcac actatggcag
     2101 ccatccatga gagtttcaaa ggttatcaac catatgaatt tactacttta aagagcttac
     2161 aagaccctat gaaaaaatta gttgagaaag aagagaagaa gaaaatcaac cagcaggaaa
     2221 gtacagatgc agccgtgcag gagccgagcc aattaatttc attagaggaa gaaaaccagc
     2281 gcaaggaatc ctctagtttt aagactgaag atggaaaaag tattttatct gccttagaca
     2341 aaggctctac acatactgca tgctcaggac ccatagatga actattagac atgaaatctg
     2401 aggaaggtgc ttgcctggga ccagtggcag ggaccccgga acctgaaggt gctgacaaag
     2461 atgacctgct gctgttgagt gagatcttca atgcttcctc cttggaagag ggcgagttca
     2521 gcaaagagtg ggccgctgtg tttggagacg gccaagtgaa ggagccagtg cccactatgg
     2581 ccctgggaga gccagacccc aaggcccaga caggctcagg tttccttcct tcgcagcttt
     2641 tagaccaaaa tatgaaagac ttacaggcct cgctacaaga acctgctaag gctgcctcag
     2701 acctgactgc ctggttcagc ctcttcgctg acctcgaccc actctcaaat cctgatgctg
     2761 ttgggaaaac cgataaagaa cacgaattgc tcaatgcatg agaattctgc agtcgacggt
     2821 accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca taccacattt
     2881 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2941 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3001 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3061 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt
     3121 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     3181 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     3241 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3301 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     3361 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     3421 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     3481 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     3541 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3601 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3661 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     3721 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3781 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3841 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     3901 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     3961 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4021 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4081 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4141 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4201 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     4261 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4321 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     4381 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     4441 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     4501 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     4561 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     4621 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     4681 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     4741 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     4801 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     4861 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     4921 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4981 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     5041 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     5101 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     5161 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     5221 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     5281 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     5341 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5401 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5461 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5521 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5581 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5641 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5701 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5761 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5821 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5881 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5941 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6001 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6061 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6121 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
