LOCUS       V032384                 7428 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032384
VERSION     V032384
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..4083
                     /label="MARS1(NM_004990)"
                     /note="MARS1(NM_004990)"
                     /gene="MARS1"
     polyA_signal    4216..4337
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4344..4799)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4826..4930
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4932..5289
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5324..6118
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6350..6397
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6726..7314
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatgagac tgttcgtgag tgatggcgtc ccgggttgct tgccggtgct ggccgccgcc
     1441 gggagagccc ggggcagagc agaggtgctc atcagcactg taggcccgga agattgtgtg
     1501 gtcccgttcc tgacccggcc taaggtccct gtcttgcagc tggatagcgg caactacctc
     1561 ttctccacta gtgcaatctg ccgatatttt tttttgttat ctggctggga gcaagatgac
     1621 ctcactaacc agtggctgga atgggaagcg acagagctgc agccagcttt gtctgctgcc
     1681 ctgtactatt tagtggtcca aggcaagaag ggggaagatg ttcttggttc agtgcggaga
     1741 gccctgactc acattgacca cagcttgagt cgtcagaact gtcctttcct ggctggggag
     1801 acagaatctc tagccgacat tgttttgtgg ggagccctat acccattact gcaagatccc
     1861 gcctacctcc ctgaggagct gagtgccctg cacagctggt tccagacact gagtacccag
     1921 gaaccatgtc agcgagctgc agagactgta ctgaaacagc aaggtgtcct ggctctccgg
     1981 ccttacctcc aaaagcagcc ccagcccagc cccgctgagg gaagggctgt caccaatgag
     2041 cctgaggagg aggagctggc taccctatct gaggaggaga ttgctatggc tgttactgct
     2101 tgggagaagg gcctagaaag tttgcccccg ctgcggcccc agcagaatcc agtgttgcct
     2161 gtggctggag aaaggaatgt gctcatcacc agtgccctcc cttacgtcaa caatgtcccc
     2221 caccttggga acatcattgg ttgtgtgctc agtgccgatg tctttgccag gtactctcgc
     2281 ctccgccagt ggaacaccct ctatctgtgt gggacagatg agtatggtac agcaacagag
     2341 accaaggctc tggaggaggg actaaccccc caggagatct gcgacaagta ccacatcatc
     2401 catgctgaca tctaccgctg gtttaacatt tcgtttgata tttttggtcg caccaccact
     2461 ccacagcaga ccaaaatcac ccaggacatt ttccagcagt tgctgaaacg aggttttgtg
     2521 ctgcaagata ctgtggagca actgcgatgt gagcactgtg ctcgcttcct ggctgaccgc
     2581 ttcgtggagg gcgtgtgtcc cttctgtggc tatgaggagg ctcggggtga ccagtgtgac
     2641 aagtgtggca agctcatcaa tgctgtcgag cttaagaagc ctcagtgtaa agtctgccga
     2701 tcatgccctg tggtgcagtc gagccagcac ctgtttctgg acctgcctaa gctggagaag
     2761 cgactggagg agtggttggg gaggacattg cctggcagtg actggacacc caatgcccag
     2821 tttatcaccc gttcttggct tcgggatggc ctcaagccac gctgcataac ccgagacctc
     2881 aaatggggaa cccctgtacc cttagaaggt tttgaagaca aggtattcta tgtctggttt
     2941 gatgccacta ttggctatct gtccatcaca gccaactaca cagaccagtg ggagagatgg
     3001 tggaagaacc cagagcaagt ggacctgtat cagttcatgg ccaaagacaa tgttcctttc
     3061 catagcttag tctttccttg ctcagcccta ggagctgagg ataactatac cttggtcagc
     3121 cacctcattg ctacagagta cctgaactat gaggatggga aattctctaa gagccgcggt
     3181 gtgggagtgt ttggggacat ggcccaggac acggggatcc ctgctgacat ctggcgcttc
     3241 tatctgctgt acattcggcc tgagggccag gacagtgctt tctcctggac ggacctgctg
     3301 ctgaagaata attctgagct gcttaacaac ctgggcaact tcatcaacag agctgggatg
     3361 tttgtgtcta agttctttgg gggctatgtg cctgagatgg tgctcacccc tgatgatcag
     3421 cgcctgctgg cccatgtcac cctggagctc cagcactatc accagctact tgagaaggtt
     3481 cggatccggg atgccttgcg cagtatcctc accatatctc gacatggcaa ccaatatatt
     3541 caggtgaatg agccctggaa gcggattaaa ggcagtgagg ctgacaggca acgggcagga
     3601 acagtgactg gcttggcagt gaatatagct gccttgctct ctgtcatgct tcagccttac
     3661 atgcccacgg ttagtgccac aatccaggcc cagctgcagc tcccacctcc agcctgcagt
     3721 atcctgctga caaacttcct gtgtacctta ccagcaggac accagattgg cacagtcagt
     3781 cccttgttcc aaaaattgga aaatgaccag attgaaagtt taaggcagcg ctttggaggg
     3841 ggccaggcaa aaacgtcccc gaagccagca gttgtagaga ctgttacaac agccaagcca
     3901 cagcagatac aagcgctgat ggatgaagtg acaaaacaag gaaacattgt ccgagaactg
     3961 aaagcacaaa aggcagacaa gaacgaggtt gctgcggagg tggcgaaact cttggatcta
     4021 aagaaacagt tggctgtagc tgaggggaaa ccccctgaag cccctaaagg caagaagaaa
     4081 aagtaaggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     4141 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4201 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4261 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4321 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     4381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4921 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     4981 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5041 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5101 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5161 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5221 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5281 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5341 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5401 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5461 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     5521 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5581 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5641 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5701 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5761 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5821 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     5881 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5941 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6001 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6061 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6121 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6181 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6241 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     6301 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     6361 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     6421 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6481 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     6541 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     6601 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6661 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6721 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6781 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6841 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6901 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6961 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7021 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7081 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7141 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7201 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7261 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7321 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7381 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
