LOCUS       V019914                 7639 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019914
VERSION     V019914
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             624..3551
                     /label="PARG(NM_003631)"
                     /note="PARG(NM_003631)"
                     /gene="PARG"
     CDS             3585..4304
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4427..4548
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4555..5010)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5037..5141
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5143..5500
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5535..6329
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6561..6608
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6937..7525
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatgaatg cgggccccgg ctgtgaaccc tgcaccaagc
      661 gaccccgctg gggcgccgct acaacttcgc cggctgcttc ggacgcccgg agctttccca
      721 gcaggcagag gcgcgtcctc gaccccaagg acgctcacgt gcagttcagg gtcccaccgt
      781 cctcgccagc ctgcgtccca gggcgggcgg gacagcacag aggcagcgcc acctcgcttg
      841 ttttcaaaca aaagactatt accagttgga tggacactaa aggaatcaag acagcggaat
      901 cagaaagttt ggatagtaaa gaaaacaaca atacaagaat agaatccatg atgagttctg
      961 tacaaaaaga taacttttac caacataatg tagaaaaatt agaaaatgtt tctcagctaa
     1021 gtcttgataa gtcacccact gaaaaaagta cacagtattt gaaccagcat cagactgcag
     1081 caatgtgtaa gtggcaaaat gaagggaaac acacggagca gcttttggaa agtgaacctc
     1141 aaacagtaac cctggtacca gagcagttta gtaatgctaa cattgatcgg tcacctcaaa
     1201 atgatgatca cagtgacaca gatagtgaag agaatagaga caatcaacag tttctcacaa
     1261 ctgtaaagct tgcaaatgca aagcagacta cagaagatga acaggccaga gaagccaaaa
     1321 gccaccagaa gtgcagcaag tcttgcgatc ctggggaaga ctgtgcaagt tgtcagcaag
     1381 atgagataga tgtggtgcca gagagtccat tgtcagatgt tggctctgag gatgttggta
     1441 ctgggccaaa aaatgacaac aaattgacta gacaagaaag ttgcctagga aattctcctc
     1501 catttgagaa ggaaagtgaa cccgagtcac cgatggatgt ggataattct aaaaatagtt
     1561 gtcaagactc agaagcagat gaggagacaa gtccaggttt tgatgaacaa gaagatggta
     1621 gttcctccca aacagcaaat aaaccttcaa ggttccaagc aagagacgct gacattgaat
     1681 ttaggaaacg gtactctact aagggcggtg aagttagatt acatttccaa tttgaaggag
     1741 gagagagtcg cactggaatg aatgatttaa atgctaaact acctggaaat atttctagcc
     1801 tgaatgtaga atgcagaaat tctaagcaac atggaaaaaa ggattctaaa atcacagatc
     1861 atttcatgag actgcccaaa gcagaggaca gaagaaaaga acagtgggaa accaaacatc
     1921 aaagaacaga aaggaagatc cctaaatacg ttccacctca cctttctcca gataagaagt
     1981 ggcttggaac tcccattgag gagatgagaa gaatgcctcg gtgtgggatc cggctgcctc
     2041 tcttgagacc atctgccaat cacacagtaa ctattcgggt agatcttttg cgagcaggag
     2101 aagttcctaa accttttcca acacattata aagatttgtg ggataacaag catgttaaaa
     2161 tgccttgttc agaacaaaat ttgtacccag tggaagatga gaatggtgag cgaactgcgg
     2221 ggagccggtg ggagctcatt cagactgcac ttctcaacaa atttacacga ccccaaaact
     2281 tgaaggatgc tattctgaaa tacaatgtgg catattctaa gaaatgggac tttacagctt
     2341 tgatcgattt ctgggataag gtacttgaag aagcagaagc tcaacattta tatcagtcca
     2401 tcttgcctga tatggtgaaa attgcactct gtctgccaaa tatttgcacc cagccaatac
     2461 cactcctgaa acagaagatg aatcattcca tcacaatgtc gcaggaacag attgccagtc
     2521 ttttagctaa tgctttcttc tgcacatttc cacgacgaaa tgctaagatg aaatcggagt
     2581 attctagtta cccagacatt aacttcaatc gattgtttga gggacgttca tcaaggaaac
     2641 cggagaaact taaaacgctc ttctgctact ttagaagagt cacagagaaa aaacctactg
     2701 ggttggtgac atttacaaga cagagtcttg aagattttcc agaatgggaa agatgtgaaa
     2761 aacccttgac acgattgcat gtcacttacg aaggtaccat agaagaaaat ggccaaggca
     2821 tgctacaggt ggattttgca aatcgttttg ttggaggtgg tgtaaccagt gcaggacttg
     2881 tgcaagaaga aatccgcttt ttaatcaatc ctgagttgat tatttcacgg ctcttcactg
     2941 aggtgctgga tcacaatgaa tgtctaatta tcacaggtac tgagcagtac agtgaataca
     3001 caggctatgc tgagacatat cgttggtccc ggagccacga agatgggagt gaaagggacg
     3061 actggcagcg gcgctgcact gagatcgttg ccatcgatgc tcttcacttc agacgctacc
     3121 tcgatcagtt tgtgcctgag aaaatgagac gcgagctgaa caaggcttac tgtggatttc
     3181 tccgtcctgg agtttcttca gagaatcttt ctgcagtggc cacaggaaac tggggctgtg
     3241 gtgcctttgg gggtgatgcc aggttaaaag ccttaataca gatattggca gctgctgcag
     3301 ctgagcgaga tgtggtttat ttcacctttg gggactcaga attgatgaga gacatttaca
     3361 gcatgcacat tttccttact gaaaggaaac tcactgttgg agatgtgtat aagctgttgc
     3421 tacgatacta caatgaagaa tgcagaaact gttccacccc tggaccagac atcaagcttt
     3481 atccattcat ataccatgct gtcgagtcct gtgcagagac cgctgaccat tcagggcaaa
     3541 ggacagggac cggaggaggg ggaagcgatc caccggtcgc caccatggtg agcaagggcg
     3601 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     3661 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     3721 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     3781 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     3841 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     3901 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     3961 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     4021 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     4081 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     4141 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     4201 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4261 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta
     4321 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     4381 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     4441 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4501 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
     4561 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     4621 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     4681 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     4741 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     4801 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     4861 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4921 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4981 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     5041 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5101 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     5161 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     5221 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5281 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5341 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5401 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5461 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     5521 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     5581 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     5641 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     5701 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     5761 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     5821 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5881 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5941 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     6001 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     6061 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     6121 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     6181 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     6241 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     6301 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     6361 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     6421 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     6481 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     6541 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     6601 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     6661 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     6721 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     6781 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6841 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7081 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7201 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7261 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7321 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7381 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7441 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7501 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7561 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7621 ccgtattacc gccatgcat
//
