LOCUS V019914 7639 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019914 VERSION V019914 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 624..3551 /label="PARG(NM_003631)" /note="PARG(NM_003631)" /gene="PARG" CDS 3585..4304 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 4427..4548 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4555..5010) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5037..5141 /gene="bla" /label="AmpR promoter" promoter 5143..5500 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5535..6329 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6561..6608 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6937..7525 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagcc accatgaatg cgggccccgg ctgtgaaccc tgcaccaagc 661 gaccccgctg gggcgccgct acaacttcgc cggctgcttc ggacgcccgg agctttccca 721 gcaggcagag gcgcgtcctc gaccccaagg acgctcacgt gcagttcagg gtcccaccgt 781 cctcgccagc ctgcgtccca gggcgggcgg gacagcacag aggcagcgcc acctcgcttg 841 ttttcaaaca aaagactatt accagttgga tggacactaa aggaatcaag acagcggaat 901 cagaaagttt ggatagtaaa gaaaacaaca atacaagaat agaatccatg atgagttctg 961 tacaaaaaga taacttttac caacataatg tagaaaaatt agaaaatgtt tctcagctaa 1021 gtcttgataa gtcacccact gaaaaaagta cacagtattt gaaccagcat cagactgcag 1081 caatgtgtaa gtggcaaaat gaagggaaac acacggagca gcttttggaa agtgaacctc 1141 aaacagtaac cctggtacca gagcagttta gtaatgctaa cattgatcgg tcacctcaaa 1201 atgatgatca cagtgacaca gatagtgaag agaatagaga caatcaacag tttctcacaa 1261 ctgtaaagct tgcaaatgca aagcagacta cagaagatga acaggccaga gaagccaaaa 1321 gccaccagaa gtgcagcaag tcttgcgatc ctggggaaga ctgtgcaagt tgtcagcaag 1381 atgagataga tgtggtgcca gagagtccat tgtcagatgt tggctctgag gatgttggta 1441 ctgggccaaa aaatgacaac aaattgacta gacaagaaag ttgcctagga aattctcctc 1501 catttgagaa ggaaagtgaa cccgagtcac cgatggatgt ggataattct aaaaatagtt 1561 gtcaagactc agaagcagat gaggagacaa gtccaggttt tgatgaacaa gaagatggta 1621 gttcctccca aacagcaaat aaaccttcaa ggttccaagc aagagacgct gacattgaat 1681 ttaggaaacg gtactctact aagggcggtg aagttagatt acatttccaa tttgaaggag 1741 gagagagtcg cactggaatg aatgatttaa atgctaaact acctggaaat atttctagcc 1801 tgaatgtaga atgcagaaat tctaagcaac atggaaaaaa ggattctaaa atcacagatc 1861 atttcatgag actgcccaaa gcagaggaca gaagaaaaga acagtgggaa accaaacatc 1921 aaagaacaga aaggaagatc cctaaatacg ttccacctca cctttctcca gataagaagt 1981 ggcttggaac tcccattgag gagatgagaa gaatgcctcg gtgtgggatc cggctgcctc 2041 tcttgagacc atctgccaat cacacagtaa ctattcgggt agatcttttg cgagcaggag 2101 aagttcctaa accttttcca acacattata aagatttgtg ggataacaag catgttaaaa 2161 tgccttgttc agaacaaaat ttgtacccag tggaagatga gaatggtgag cgaactgcgg 2221 ggagccggtg ggagctcatt cagactgcac ttctcaacaa atttacacga ccccaaaact 2281 tgaaggatgc tattctgaaa tacaatgtgg catattctaa gaaatgggac tttacagctt 2341 tgatcgattt ctgggataag gtacttgaag aagcagaagc tcaacattta tatcagtcca 2401 tcttgcctga tatggtgaaa attgcactct gtctgccaaa tatttgcacc cagccaatac 2461 cactcctgaa acagaagatg aatcattcca tcacaatgtc gcaggaacag attgccagtc 2521 ttttagctaa tgctttcttc tgcacatttc cacgacgaaa tgctaagatg aaatcggagt 2581 attctagtta cccagacatt aacttcaatc gattgtttga gggacgttca tcaaggaaac 2641 cggagaaact taaaacgctc ttctgctact ttagaagagt cacagagaaa aaacctactg 2701 ggttggtgac atttacaaga cagagtcttg aagattttcc agaatgggaa agatgtgaaa 2761 aacccttgac acgattgcat gtcacttacg aaggtaccat agaagaaaat ggccaaggca 2821 tgctacaggt ggattttgca aatcgttttg ttggaggtgg tgtaaccagt gcaggacttg 2881 tgcaagaaga aatccgcttt ttaatcaatc ctgagttgat tatttcacgg ctcttcactg 2941 aggtgctgga tcacaatgaa tgtctaatta tcacaggtac tgagcagtac agtgaataca 3001 caggctatgc tgagacatat cgttggtccc ggagccacga agatgggagt gaaagggacg 3061 actggcagcg gcgctgcact gagatcgttg ccatcgatgc tcttcacttc agacgctacc 3121 tcgatcagtt tgtgcctgag aaaatgagac gcgagctgaa caaggcttac tgtggatttc 3181 tccgtcctgg agtttcttca gagaatcttt ctgcagtggc cacaggaaac tggggctgtg 3241 gtgcctttgg gggtgatgcc aggttaaaag ccttaataca gatattggca gctgctgcag 3301 ctgagcgaga tgtggtttat ttcacctttg gggactcaga attgatgaga gacatttaca 3361 gcatgcacat tttccttact gaaaggaaac tcactgttgg agatgtgtat aagctgttgc 3421 tacgatacta caatgaagaa tgcagaaact gttccacccc tggaccagac atcaagcttt 3481 atccattcat ataccatgct gtcgagtcct gtgcagagac cgctgaccat tcagggcaaa 3541 ggacagggac cggaggaggg ggaagcgatc caccggtcgc caccatggtg agcaagggcg 3601 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 3661 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 3721 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga 3781 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca 3841 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 3901 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 3961 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 4021 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 4081 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 4141 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt 4201 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 4261 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta 4321 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 4381 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 4441 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 4501 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg 4561 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 4621 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 4681 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 4741 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 4801 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 4861 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 4921 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 4981 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg 5041 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 5101 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca 5161 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 5221 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 5281 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 5341 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 5401 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 5461 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat 5521 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 5581 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 5641 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 5701 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 5761 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 5821 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 5881 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 5941 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 6001 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 6061 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 6121 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 6181 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 6241 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 6301 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 6361 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 6421 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 6481 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc 6541 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc 6601 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag 6661 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc 6721 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg 6781 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 6841 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 6901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 6961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 7021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 7081 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 7141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 7201 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 7261 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 7321 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 7381 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 7441 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 7501 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 7561 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 7621 ccgtattacc gccatgcat //