LOCUS       V037737                11462 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037737
VERSION     V037737
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1378..8022
                     /label="PTPRB(NM_001109754)"
                     /note="PTPRB(NM_001109754)"
                     /gene="PTPRB"
     CDS             8029..8118
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     polyA_signal    8250..8371
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(8378..8833)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8860..8964
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        8966..9323
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9358..10152
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10384..10431
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      10760..11348
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tggaggtgga ggtagtggag gaggcggaag caagcttatg
     1381 gaggctgaat tttacatggt gattcttacc tgcttgatct tcaggaactc agaagggttt
     1441 cagattgtcc atgtccagaa acaacagtgt cttttcaaaa atgagaaagt ggtcgtgggc
     1501 tcatgcaaca ggaccatcca gaaccagcag tggatgtgga ctgaggatga aaagctcctt
     1561 catgttaaat ctgcactgtg cttggccatc tccaactctt cccgcggccc ctcccgctca
     1621 gccatcttgg accgctgttc ccaggcaccc cgatggacct gctatgatca ggaaggcttc
     1681 cttgaggtgg aaaatgcctc tctctttctc cagaaacaag gctccagagt agtggtcaag
     1741 aaggccagga aatacctcca tagctggatg aaaatagatg tcaacaagga gggaaaactg
     1801 gtcaatgaaa gcctctgttt acaaaaagct ggcctgggag cagaagtttc ggtgaggagc
     1861 actagaaaca cggctccacc ccagattctc actaccttta atgcagttcc agatggcctg
     1921 gtattcctta ttaggaatac cacagaggcc ttcatcagaa atgctgcaga aaactacagc
     1981 caaaacagca gcgagaggca gcatcccaat ctgcacatga ctggaattac agacacatca
     2041 tgggttttgt cgactactca gcccttctcc agcaccactg aagagactgg actggcggag
     2101 ccagagagat gtaacttcac cctggcggag tccaaggcct ccagccattc tgtgtctatc
     2161 cagtggagaa ttttgggctc accctgtaac tttagcctca tctatagcag tgacaccctg
     2221 ggggccgcgt tgtgccctac ctttcggata gacaacacca catacggatg taaccttcaa
     2281 gatttacaag caggaaccat ctataacttc aggattattt ctctggatga agagagaaca
     2341 gtggtcttgc aaacagatcc tttacctcct gctaggtttg gagtcagtaa agagaagacg
     2401 acttcaacca gcttgcatgt ttggtggact ccttcttccg gaaaagtcac ctcatatgag
     2461 gtgcaattat ttgatgaaaa taaccaaaag atacaggggg ttcaaattca agaaagtact
     2521 tcatggaatg aatacacttt tttcaatctc actgctggta gtaaatacaa tattgccatc
     2581 acagctgttt ctggaggaaa acgttctttt tcagtttata ccaatggatc aacagtgcca
     2641 tctccagtga aagatattgg tatttccaca aaagccaatt ctctcctgat ttcctggtcc
     2701 catggttctg ggaatgtgga acgataccgg ctgatgctaa tggataaagg gatcctagtt
     2761 catggcggtg ttgtggacaa acatgctact tcctatgctt ttcacgggct gacccctggc
     2821 tacctctaca acctcactgt tatgactgag gctgcagggc tgcaaaacta caggtggaaa
     2881 ctagtcagga cagcccccat ggaagtctca aatctgaagg tgacaaatga tggcagtttg
     2941 acctctctaa aagtcaaatg gcaaagacct cctggaaatg tggattctta caatatcacc
     3001 ctgtctcaca aagggaccat caaggaatcc agagtattag caccttggat tactgaaact
     3061 cactttaaag agttagtccc cggtcgactt tatcaagtta ctgtcagctg tgtctctggt
     3121 gaactgtctg ctcagaagat ggcagtgggc agaacatttc cagacaaagt tgcaaacctg
     3181 gaggcaaaca ataatggcag gatgaggtct cttgtagtga gctggtcgcc ccctgctgga
     3241 gactgggagc agtatcggat cctactcttc aatgattctg tggtgctgct caacatcact
     3301 gtgggaaagg aagaaacaca gtatgtcatg gatgacacgg ggctcgtacc gggaagacag
     3361 tatgaggtgg aagtcattgt tgagagtgga aatttgaaga attctgagcg ttgccaaggc
     3421 aggacagtcc ccctggctgt cctccagctt cgtgtcaaac atgccaatga aacctcactg
     3481 agtatcatgt ggcagacccc tgtagcagaa tgggagaaat acatcatttc cctagctgac
     3541 agagacctct tactgatcca caagtcactc tccaaagatg ccaaagaatt cacttttact
     3601 gacctggtgc ctggacgaaa atacatggct acagtcacca gtattagtgg agacttaaaa
     3661 aattcctctt cagtaaaagg aagaacagtg cctgcccaag tgactgactt gcatgtggcc
     3721 aaccaaggaa tgaccagtag tctgtttact aactggaccc aggcacaagg agacgtagaa
     3781 ttttaccaag tcttactgat ccatgaaaat gtggtcatta aaaatgaaag catctccagt
     3841 gagaccagca gatacagctt ccactctctc aagtccggca gcctgtactc cgtggtggta
     3901 acaacagtga gtggagggat ctcttcccga caagtggttg tggagggaag aacagtccct
     3961 tccagtgtga gtggagtaac ggtgaacaat tccggtcgta atgactacct cagcgtttcc
     4021 tggctgctgg cgcccggaga tgtggataac tatgaggtaa cattgtctca tgacggcaag
     4081 gtggttcagt cccttgtcat tgccaagtct gtcagagaat gttccttcag ctccctcacc
     4141 ccaggccgcc tctacaccgt gaccataact acaaggagtg gcaagtatga aaatcactcc
     4201 ttcagccaag agcggacagt gcctgacaaa gtccagggag tcagtgttag caactcagcc
     4261 aggagtgact atttaagggt atcctgggtg catgccactg gagactttga tcactatgaa
     4321 gtcaccatta aaaacaaaaa caacttcatt caaactaaaa gcattcccaa gtcagaaaac
     4381 gaatgtgtat ttgttcagct agtccctgga cggttgtaca gtgtcactgt tactacaaaa
     4441 agtggacaat atgaagccaa tgaacaaggg aatgggagaa caattccaga gcctgttaag
     4501 gatctaacat tgcgcaacag gagcactgag gacttgcatg tgacttggtc aggagctaat
     4561 ggggatgtcg accaatatga gatccagctg ctcttcaatg acatgaaagt atttcctcct
     4621 tttcaccttg taaataccgc aaccgagtat cgatttactt ccctaacacc aggccgccaa
     4681 tacaaaattc ttgtcttgac gattagcggg gatgtacagc agtcagcctt cattgagggc
     4741 ttcacagttc ctagtgctgt caaaaatatt cacatttctc ccaatggagc aacagatagc
     4801 ctgacggtga actggactcc tggtggggga gacgttgatt cctacacggt gtcggcattc
     4861 aggcacagtc aaaaggttga ctctcagact attcccaagc acgtctttga gcacacgttc
     4921 cacagactgg aggccgggga gcagtaccag atcatgattg cctcagtcag cgggtccctg
     4981 aagaatcaga taaatgtggt tgggcggaca gttccagcat ctgtccaagg agtaattgca
     5041 gacaatgcat acagcagtta ttccttaata gtaagttggc aaaaagctgc tggtgtggca
     5101 gaaagatatg atatcctgct tctaactgaa aatggaatcc ttctgcgcaa cacatcagag
     5161 ccagccacca ctaagcaaca caaatttgaa gatctaacac caggcaagaa atacaagata
     5221 cagatcctaa ctgtcagtgg aggcctcttt agcaaggaag cccagactga aggccgaaca
     5281 gtcccagcag ctgtcaccga cctgaggatc acagagaact ccaccaggca cctgtccttc
     5341 cgctggaccg cctcagaggg ggagctcagc tggtacaaca tctttttgta caacccagat
     5401 gggaatctcc aggagagagc tcaagttgac ccactagtcc agagcttctc tttccagaac
     5461 ttgctacaag gcagaatgta caagatggtg attgtaactc acagtgggga gctgtctaat
     5521 gagtctttca tatttggtag aacagtccca gcctctgtga gtcatctcag ggggtccaat
     5581 cggaacacga cagacagcct ttggttcaac tggagtccag cctctgggga ctttgacttt
     5641 tatgagctga ttctctataa tcccaatggc acaaagaagg aaaactggaa agacaaggac
     5701 ctgacggagt ggcggtttca aggccttgtt cctggaagga agtacgtgct gtgggtggta
     5761 actcacagtg gagatctcag caataaagtc acagcggaga gcagaacagc tccaagtcct
     5821 cccagtctta tgtcatttgc tgacattgca aacacatcct tggccatcac gtggaaaggg
     5881 cccccagact ggacagacta caacgacttt gagctgcagt ggttgcccag agatgcactt
     5941 actgtcttca acccctacaa caacagaaaa tcagaaggac gcattgtgta tggtcttcgt
     6001 ccagggagat cctatcaatt caacgtcaag actgtcagtg gtgattcctg gaaaacttac
     6061 agcaaaccaa tttttggatc tgtgaggaca aagcctgaca agatacaaaa cctgcattgc
     6121 cggcctcaga actccacggc cattgcctgt tcttggatcc ctcctgattc tgactttgat
     6181 ggttatagta ttgaatgccg gaaaatggac acccaagaag ttgagttttc cagaaagctg
     6241 gagaaagaaa aatctctgct caacatcatg atgctagtgc cccataagag gtacctggtg
     6301 tccatcaaag tgcagtcggc cggcatgacc agcgaggtgg ttgaagacag cactatcaca
     6361 atgatagacc gcccccctcc tccaccccca cacattcgtg tgaatgaaaa ggatgtgcta
     6421 attagcaagt cttccatcaa ctttactgtc aactgcagct ggttcagcga caccaatgga
     6481 gctgtgaaat acttcacagt ggtggtgaga gaggctgatg gcagtgatga gctgaagcca
     6541 gaacagcagc accctctccc ttcctacctg gagtacaggc acaatgcctc cattcgggtg
     6601 tatcagacta attattttgc cagcaaatgt gccgaaaatc ctaacagcaa ctccaagagt
     6661 tttaacatta agcttggagc agagatggag agcctaggtg gaaaatgcga tcccactcag
     6721 caaaaattct gtgatggacc actgaagcca cacactgcct acagaatcag cattcgagct
     6781 tttacacagc tctttgatga ggacctgaag gaattcacaa agccactcta ttcagacaca
     6841 tttttttctt tacccatcac tactgaatca gagcccttgt ttggagctat tgaaggtgtg
     6901 agtgctggtc tgtttttaat tggcatgcta gtggctgttg ttgccttatt gatctgcaga
     6961 cagaaagtga gccatggtcg agaaagaccc tctgcccgtc tgagcattcg tagggatcga
     7021 ccattatctg tccacttaaa cctgggccag aaaggtaacc ggaaaacttc ttgtccaata
     7081 aaaataaatc agtttgaagg gcatttcatg aagctacagg ctgactccaa ctaccttcta
     7141 tccaaggaat acgaggagtt aaaagacgtg ggccgaaacc agtcatgtga cattgcactc
     7201 ttgccggaga atagagggaa aaatcgatac aacaatatat tgccctatga tgccacgcga
     7261 gtgaagctct ccaatgtaga tgatgatcct tgctctgact acatcaatgc cagctacatc
     7321 cctggcaaca acttcagaag agaatacatt gtcactcagg gaccgcttcc tggcaccaag
     7381 gatgacttct ggaaaatggt gtgggaacaa aacgttcaca acatcgtcat ggtgacccag
     7441 tgtgttgaga agggccgagt aaagtgtgat cattactggc cagcggacca ggattccctc
     7501 tactatgggg acctcatcct gcagatgctc tcagagtccg tcctgcctga gtggaccatc
     7561 cgggagttta agatatgcgg tgaggaacag cttgatgcac acagactcat ccgccacttt
     7621 cactatacgg tgtggccaga ccatggagtc ccagaaacca cccagtctct gatccagttt
     7681 gtgagaactg tcagggacta catcaacaga agcccgggtg ctgggcccac tgtggtgcac
     7741 tgcagtgctg gtgtgggtag gactggaacc tttattgcat tggaccgaat cctccagcag
     7801 ttagactcca aagactctgt ggacatttat ggagcagtgc acgacctaag acttcacagg
     7861 gttcacatgg tccagactga gtgtcagtat gtctacctac atcagtgtgt aagagatgtc
     7921 ctcagagcaa gaaagctacg gagtgaacaa gaaaacccct tgtttccaat ctatgaaaat
     7981 gtgaatccag agtatcacag agatccagtc tattcaaggc atgaattcga acaaaaacta
     8041 atatcagagg aagatttgga acagaaacta atcagtgaag aagatttaga gcaaaagtta
     8101 atttccgagg aggacttata ggatccaccg gatctagata actgatcata atcagccata
     8161 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     8221 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     8281 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8341 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg
     8401 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     8461 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     8521 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     8581 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     8641 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     8701 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     8761 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     8821 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8881 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8941 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     9001 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9061 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9121 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     9181 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     9241 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     9301 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     9361 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     9421 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     9481 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     9541 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     9601 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     9661 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     9721 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     9781 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     9841 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     9901 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     9961 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
    10021 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
    10081 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
    10141 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
    10201 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
    10261 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
    10321 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
    10381 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
    10441 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
    10501 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
    10561 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
    10621 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10681 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    10921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11341 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11401 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
    11461 at
//
