LOCUS V021613 5808 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021613 VERSION V021613 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers rep_origin join(5804..5808,1..584) /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" enhancer 759..1062 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 1063..1266 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 1311..2027 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 2034..3101 /label="SERTAD4(NM_019605)" /note="SERTAD4(NM_019605)" /gene="SERTAD4" CDS 3117..3146 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" polyA_signal 3294..3415 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3422..3877) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3904..4008 /gene="bla" /label="AmpR promoter" promoter 4010..4367 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4402..5196 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5428..5475 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" ORIGIN 1 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 61 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 121 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 181 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 241 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 301 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 361 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 421 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 481 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 541 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 601 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 661 cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt 721 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 781 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 841 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 901 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 961 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 1021 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 1081 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 1141 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 1201 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 1261 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagca 1321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 1381 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 1441 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 1501 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 1561 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 1621 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 1681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 1741 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 1801 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 1861 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 1921 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 1981 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggcc accatgactc 2041 tggttctgtc catgaataga ttctgcgagc ccattgtctc ggaaggagct gctgaaattg 2101 ctgggtacca aacactatgg gaggctgaca gctacggagg cccaagcccc ccagggccag 2161 cacaagctcc tttgcaggga gaccggggag ctggtccccc actggcagga tcacattaca 2221 ggggaatttc aaatcctata acaacatcca agatcacata ctttaagagg aagtatgtgg 2281 aagaagagga ttttcaccca ccactcagca gctgtagcca taaaaccatc tcaatttttg 2341 aggaacgagc ccacatcctt tatatgtcct tagaaaagct aaagtttatc gatgatcctg 2401 aagtgtacct ccgaagatct gtccttataa acaatttgat gaaaaggatc catggagaaa 2461 ttatcatgca gaataactgg tgcttccctg cctgctcttt caatggcacc tctgcccaag 2521 agtggtttat ggctcaagac tgcccttacc gaaaacgacc acggatggcc aaagaggaat 2581 gtgaaaagtt tcatgcctgc tgcttttacc aagaatgtgg tggccactac ctaaatttac 2641 ccctttctgt caatgctaat gttggaagtg cctccactgc tgcctcctct ccctccgcct 2701 cttcttcctc ctcatcttcc tcttcctctc cccctttgcc tttaccgagt tgttcccgcc 2761 aggtggattt tgatgtaggt agtgcatcta tttacaagag tgatggccag atacctgcca 2821 atgaaatctt tgtcactaat gtcagatcac ttggtgttca ggaaaaggcc aaattaaatg 2881 atgagaaagc aaatgatgac accaacagag atggtggccc cctcagccac gaacctgtgg 2941 gaaatgacct tgcttttgag tgcaaaggcc aattttatga ttattttgag accggatata 3001 atgaaagaaa caatgtaaat gaatcttgga aaaagtcctt acggaaaaag gaggcttcac 3061 caccaagtaa caaactgtgc tgcagcaaag gaagtaaaat atccggactc agatctgagc 3121 agaaactcat ctctgaagag gatctgtaag gtaccgcggg cccgggatcc accggatcta 3181 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 3241 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 3301 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 3361 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg 3421 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 3481 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 3541 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 3601 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 3661 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 3721 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 3781 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 3841 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 3901 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 3961 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 4021 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 4081 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 4141 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 4201 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 4261 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 4321 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 4381 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 4441 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 4501 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 4561 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 4621 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 4681 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 4741 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 4801 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 4861 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 4921 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 4981 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 5041 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 5101 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 5161 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 5221 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 5281 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 5341 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 5401 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 5461 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 5521 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 5581 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 5641 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 5701 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 5761 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgag //