LOCUS       V042243                 6745 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042243
VERSION     V042243
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1378..3369
                     /label="SLC5A1(NM_000343)"
                     /note="SLC5A1(NM_000343)"
                     /gene="SLC5A1"
     polyA_signal    3533..3654
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3661..4116)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4143..4247
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4249..4606
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4641..5435
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5667..5714
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6043..6631
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggt ggaggcggat caggaggcgg tggatctatg
     1381 gacagtagca cctggagccc caagaccacc gcggtcaccc ggcctgttga gacccacgag
     1441 ctcattcgca atgcagccga tatctccatc atcgttatct acttcgtggt agtgatggcc
     1501 gtcggactgt gggctatgtt ttccaccaat cgtgggactg ttggaggctt cttcctggca
     1561 ggccgaagta tggtgtggtg gccgattgga gcctccctct ttgctagtaa cattggaagt
     1621 ggccactttg tggggctggc cgggactggg gcagcttcag gcatcgccat tggaggcttt
     1681 gaatggaatg ccctggtttt ggtggttgtg ctgggctggc tgtttgtccc catctatatt
     1741 aaggctgggg tggtgacaat gccagagtac ctgaggaagc ggtttggagg ccagcggatc
     1801 caggtctacc tttcccttct gtccctgctg ctctacattt tcaccaagat ctcggcagac
     1861 atcttctcgg gggccatatt catcaatctg gccttaggcc tgaatctgta tttagccatc
     1921 tttctcttat tggcaatcac tgccctttac acaattacag ggggcctggc ggcggtgatt
     1981 tacacggaca ccttgcagac ggtgatcatg ctggtggggt ctttaatcct gactgggttt
     2041 gcttttcacg aagtgggagg ctatgacgcc ttcatggaaa agtacatgaa agccattcca
     2101 accatagtgt ctgatggcaa caccaccttt caggaaaaat gctacactcc aagggccgac
     2161 tccttccaca tcttccgaga tcccctcacg ggagacctcc catggcctgg gttcatcttt
     2221 gggatgtcca tccttacctt gtggtactgg tgcacagatc aggtcattgt gcagcgctgc
     2281 ctctcagcca agaatatgtc tcacgtgaag ggtggctgca tcctgtgtgg gtatctaaag
     2341 ctgatgccca tgttcatcat ggtgatgcca ggaatgatca gccgcattct gtacacagaa
     2401 aaaattgcct gtgtcgtccc ttcagaatgt gagaaatatt gcggtaccaa ggttggctgt
     2461 accaacatcg cctatccaac cttagtggtg gagctcatgc ccaatggact gcgaggcctg
     2521 atgctatcag tcatgctggc ctccctcatg agctccctga cctccatctt caacagcgcc
     2581 agcaccctct tcaccatgga catctacgcc aaggtccgca agagagcatc tgagaaagag
     2641 ctcatgattg ccggaaggtt gtttatcctg gtgctgattg gcatcagcat cgcctgggtg
     2701 cccattgtgc agtcagcaca aagtgggcaa ctcttcgatt acatccagtc catcaccagt
     2761 tacttgggac cacccattgc ggctgtcttc ctgcttgcta ttttctggaa gagagtcaat
     2821 gagccaggag ccttttgggg actgatccta ggacttctga ttgggatttc acgtatgatt
     2881 actgagtttg cttatggaac cgggagctgc atggagccca gcaactgtcc cacgattatc
     2941 tgtggggtgc actacttgta ctttgccatt atcctcttcg ccatttcttt catcaccatc
     3001 gtggtcatct ccctcctcac caaacccatt ccggatgtgc atctctaccg tctgtgttgg
     3061 agcctgcgca acagcaaaga ggagcgtatt gacctggatg cggaagagga gaacatccaa
     3121 gaaggcccta aggagaccat tgaaatagaa acacaagttc ctgagaagaa aaaaggaatc
     3181 ttcaggagag cctatgacct attttgtggg ctagagcagc acggtgcacc caagatgact
     3241 gaggaagagg agaaagccat gaagatgaag atgacggaca cctctgagaa gcctttgtgg
     3301 aggacagtgt tgaacgtcaa tggcatcatc ctggtgaccg tggctgtctt ttgccatgca
     3361 tattttgcct gagaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag
     3421 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3481 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3541 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3601 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
     3661 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3721 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3781 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3841 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3901 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     3961 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     4021 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     4081 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     4141 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4201 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     4261 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4321 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4381 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4441 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4501 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4561 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     4621 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     4681 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4741 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4801 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     4861 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     4921 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4981 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     5041 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     5101 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     5161 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     5221 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     5281 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     5341 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5401 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     5461 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     5521 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5581 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     5641 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     5701 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     5761 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     5821 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     5881 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5941 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6001 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6061 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6121 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6181 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6241 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6301 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6361 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6421 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6481 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6541 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6601 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6661 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6721 ggataaccgt attaccgcca tgcat
//
