LOCUS       V018484                 5769 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018484
VERSION     V018484
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1305
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             1342..2409
                     /label="SQSTM1(NM_001142298)"
                     /note="SQSTM1(NM_001142298)"
                     /gene="SQSTM1"
     polyA_signal    2557..2678
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2685..3140)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3167..3271
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3273..3630
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3665..4459
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4691..4738
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5067..5655
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatgagcga gctgatcaag gagaacatgc acatgaagct gtacatggag
      661 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      721 ggcacccaga ccatgaagat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
      781 atcctggcta ccagcttcat gtacggcagc aaagccttca tcaaccacac ccagggcatc
      841 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagaat caccacatac
      901 gaagacgggg gcgtgctgac cgctacccag gacaccagct tccagaacgg ctgcatcatc
      961 tacaacgtca agatcaacgg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     1021 acacgcggct gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctgagaggc
     1081 cacagccaga tggccctgaa gctcgtgggc gggggctacc tgcactgctc cttcaagacc
     1141 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcttcca cttcgtggac
     1201 cacagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgagatg
     1261 gctgtggcca agtactgcga cctccctagc aaactggggc acagatccgg actcagatct
     1321 cgagctcaag cttcgaattc gatggccatg tcctacgtga aggatgacat cttccgaatc
     1381 tacattaaag agaaaaaaga gtgccggcgg gaccaccgcc caccgtgtgc tcaggaggcg
     1441 ccccgcaaca tggtgcaccc caatgtgatc tgcgatggct gcaatgggcc tgtggtagga
     1501 acccgctaca agtgcagcgt ctgcccagac tacgacttgt gtagcgtctg cgagggaaag
     1561 ggcttgcacc gggggcacac caagctcgca ttccccagcc ccttcgggca cctgtctgag
     1621 ggcttctcgc acagccgctg gctccggaag gtgaaacacg gacacttcgg gtggccagga
     1681 tgggaaatgg gtccaccagg aaactggagc ccacgtcctc ctcgtgcagg ggaggcccgc
     1741 cctggcccca cggcagaatc agcttctggt ccatcggagg atccgagtgt gaatttcctg
     1801 aagaacgttg gggagagtgt ggcagctgcc cttagccctc tgggcattga agttgatatc
     1861 gatgtggagc acggagggaa aagaagccgc ctgacccccg tctctccaga gagttccagc
     1921 acagaggaga agagcagctc acagccaagc agctgctgct ctgaccccag caagccgggt
     1981 gggaatgttg agggcgccac gcagtctctg gcggagcaga tgaggaagat cgccttggag
     2041 tccgaggggc gccctgagga acagatggag tcggataact gttcaggagg agatgatgac
     2101 tggacccatc tgtcttcaaa agaagtggac ccgtctacag gtgaactcca gtccctacag
     2161 atgccagaat ccgaagggcc aagctctctg gacccctccc aggagggacc cacagggctg
     2221 aaggaagctg ccttgtaccc acatctcccg ccagaggctg acccgcggct gattgagtcc
     2281 ctctcccaga tgctgtccat gggcttctct gatgaaggcg gctggctcac caggctcctg
     2341 cagaccaaga actatgacat cggagcggct ctggacacca tccagtattc aaagcatccc
     2401 ccgccgttgt gaggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
     2461 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     2521 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     2581 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     2641 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
     2701 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     2761 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     2821 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     2881 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     2941 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     3001 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     3061 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     3121 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3181 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3241 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     3301 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3361 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3421 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3481 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3541 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3601 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     3661 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     3721 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     3781 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     3841 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     3901 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     3961 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4021 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4081 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4141 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     4201 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4261 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     4321 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     4381 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     4441 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     4501 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     4561 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     4621 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     4681 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     4741 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     4801 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     4861 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     4921 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4981 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5041 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5101 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5161 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5221 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5281 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5341 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5401 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5461 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5521 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5581 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5641 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5701 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5761 gccatgcat
//
