LOCUS       V019491                 6912 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019491
VERSION     V019491
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1354..3567
                     /label="SUN2(NM_001199579)"
                     /note="SUN2(NM_001199579)"
                     /gene="SUN2"
     polyA_signal    3700..3821
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3828..4283)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4310..4414
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4416..4773
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4808..5602
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5834..5881
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6210..6798
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagg aggaggggga agcatgtccc gaagaagcca gcgcctcacg
     1381 cgctactccc agggtgacga tgacggcagc agcagcagcg gagggagctc ggtggctggg
     1441 agtcagagca ccctgtttaa agacagtcct ctcaggacct tgaagaggaa atccagcaac
     1501 atgaagcgcc tgtccccagc gccacagctg ggcccgtcct ctgatgcaca cacctcctac
     1561 tacagtgagt cgctggtcca cgagtcctgg ttcccaccca ggagctccct ggaggaactg
     1621 catggtgacg ccaactgggg tgaggacctg cgggtgcgga ggaggagagg cacgggtggc
     1681 tcagagagca gcagggccag cgggcttgtg gggcgcaagg ccaccgagga cttcctgggc
     1741 tcttcctcgg gctactcctc tgaggacgac tacgtggaag acagtgaagg aagaggctcg
     1801 aaagtgacag agactgagcc agtgtcctcg ttccccgcag gctactcgga tgtggaccag
     1861 cagagttcca gctcgcggct ccgaagcgcc gtctcacggg cgggctcctt actctggatg
     1921 gtggccactt cgccaggccg gctcttcaga cttctctact ggtgggctgg caccacctgg
     1981 taccgcctga ccacagctgc ctccctcctt gacgtcttcg ttttaaccag gcgcttctcg
     2041 tccctgaaga cgttcctctg gttcctgctg ccgctgctct tgctgacgtg cctgacgtat
     2101 ggtgcttggt atttctaccc ctatgggctg cagacattcc accctgcttt ggtttcctgg
     2161 tgggcagcga aggacagcag gaggccggat gagggctggg aagccagaga ctcatcgcca
     2221 catttccagg ctgagcagcg tgttatgtcc cgggtacact ctctggagcg gcgtctggaa
     2281 gctcttgctg ctgaattttc ctccaactgg cagaaggagg ccatgcggct ggaacgtctg
     2341 gagctgcggc aaggggctcc tggccaggga ggtggtggtg gcctgagcca cgaggacacc
     2401 ctggcgctgc tggaggggct agtgagccgc cgtgaagctg ccctgaagga ggatttccgc
     2461 agggaaactg ctgctcgcat ccaggaagaa ctgtctgccc tgagagcaga gcatcagcaa
     2521 gactcagaag acctcttcaa gaagatcgtc cgggcctccc aggagtccga ggctcgcatc
     2581 cagcagctga agtcagagtg gcaaagcatg acccaggagt ccttccagga gagctctgtg
     2641 aaggagctga ggcggctgga ggaccagctg gccggcctgc agcaggagct ggcggctctg
     2701 gcactgaagc agagctcggt ggcggaagaa gtgggcctgc tgccccagca gatccaggcc
     2761 gtgcgggacg acgtggaatc tcagttcccg gcctggatca gtcagttcct tgcccgaggt
     2821 ggagggggcc gcgtggggct ccttcagaga gaggagatgc aagctcagct gcgagagctg
     2881 gagagcaaga tcctcaccca tgtggcagag atgcagggca agtcggccag ggaagccgcg
     2941 gcctccctga gcctgacgct gcagaaagaa ggtgtgattg gagtgacaga ggagcaggtg
     3001 caccacatcg tgaagcaggc cctgcagcgc tacagtgagg accgcatcgg gctggcagac
     3061 tacgccctgg agtcaggagg ggccagcgtc atcagcaccc gatgttctga gacctacgag
     3121 accaagacgg ccctcctcag cctcttcggc atccccctgt ggtaccactc ccagtcaccc
     3181 cgagtcatcc tccagccaga tgtgcaccca ggcaactgct gggccttcca ggggccacaa
     3241 ggcttcgccg tggtccgcct ctctgcccgc atccgcccca cagccgttac cttagagcat
     3301 gtgcccaagg ccttgtcacc caacagcact atctccagtg cccccaagga cttcgccatc
     3361 tttgggtttg acgaagacct gcagcaggag gggacactcc ttggcaagtt cacttacgat
     3421 caggacggcg agcctattca gacgtttcac tttcaggccc ctacgatggc cacgtaccag
     3481 gtggtggagc tgcggatcct gactaactgg ggccaccccg agtacacctg catctaccgc
     3541 ttcagagtgc atggggagcc cgcccactag ggatccaccg gatctagata actgatcata
     3601 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3661 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3721 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3781 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
     3841 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3901 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3961 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     4021 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     4081 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     4141 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     4201 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     4261 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4321 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4381 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     4441 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4501 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4561 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4621 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4681 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4741 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     4801 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4861 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4921 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4981 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5041 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5101 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5161 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5221 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5281 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     5341 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5401 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5461 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5521 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5581 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     5641 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5701 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5761 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     5821 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     5881 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     5941 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     6001 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     6061 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6121 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     6361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6841 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6901 accgccatgc at
//
