LOCUS V019955 5146 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019955 VERSION V019955 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 624..1058 /label="SYNJ2BP(NM_018373)" /note="SYNJ2BP(NM_018373)" /gene="SYNJ2BP" CDS 1092..1811 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 1934..2055 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2062..2517) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2544..2648 /gene="bla" /label="AmpR promoter" promoter 2650..3007 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3042..3836 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4068..4115 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4444..5032 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagcc accatgaacg gaagagtgga ttatttggtc actgaggaag 661 agatcaatct taccagaggg ccctcagggc tgggcttcaa catcgtcggt gggacagatc 721 agcagtatgt ctccaacgac agtggcatct acgtcagccg catcaaagaa aatggggctg 781 cggccctgga tgggcggctc caggagggtg ataagatcct ttcggtaaat ggccaagacc 841 taaagaacct gctgcaccag gatgctgtag acctctttcg taatgcaggc tatgctgtgt 901 ctctgagagt gcagcacagg ttacaggtgc agaatggacc tataggacat cgaggtgaag 961 gggacccaag tggtattccc atatttatgg tgctggtgcc agtgtttgcc ctcaccatgg 1021 tagcagcctg ggctttcatg agataccggc aacaacttgg aggaggggga agcgatccac 1081 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 1141 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 1201 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 1261 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 1321 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 1381 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 1441 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 1501 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 1561 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 1621 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 1681 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 1741 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 1801 tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt 1861 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 1921 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 1981 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 2041 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 2101 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 2161 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 2221 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 2281 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 2341 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 2401 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 2461 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 2521 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 2581 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 2641 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 2701 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 2761 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 2821 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 2881 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 2941 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 3001 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 3061 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 3121 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 3181 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 3241 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 3301 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 3361 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 3421 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 3481 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 3541 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 3601 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 3661 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 3721 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 3781 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 3841 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 3901 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 3961 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct 4021 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 4081 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg 4141 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc 4201 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg 4261 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact 4321 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 4381 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 4441 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 4501 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 4561 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 4621 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4681 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 4741 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 4801 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 4861 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 4921 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 4981 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 5041 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 5101 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat //