LOCUS       V037522                 6882 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037522
VERSION     V037522
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..3537
                     /label="TARS2(NM_025150)"
                     /note="TARS2(NM_025150)"
                     /gene="TARS2"
     polyA_signal    3670..3791
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3798..4253)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4280..4384
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4386..4743
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4778..5572
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5804..5851
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6180..6768
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatggccc tgtatcagag gtggcggtgt ctccggctcc aaggtttaca ggcttgcagg
     1441 ctacacacgg cagttgtgtc gacccctcca cgctggttgg cagagcggct tggccttttt
     1501 gaggagctgt gggctgctca ggtaaagaga ttagcaagca tggcacagaa ggaaccccgg
     1561 actattaaga tatcacttcc tggaggccag aaaattgatg ctgtggcatg gaacacaacc
     1621 ccctaccaac tagcccggca gatcagttca acactggcag atactgcagt ggctgctcaa
     1681 gtgaatggag aaccttatga tctggagcgg cccttggaga cagattctga cctcagattt
     1741 ctgacattcg attccccaga ggggaaagca gtgttctggc actccagcac ccatgtcctg
     1801 ggggcagcag ctgaacaatt cctaggtgct gttctctgca gaggtccaag tacagaatat
     1861 ggcttttacc atgatttctt cctgggaaag gagaggacaa tccggggctc agagctgcct
     1921 gttttggagc ggatttgcca ggaacttaca gctgctgctc gacccttccg gaggctagag
     1981 gcttcacggg atcagcttcg ccagttgttc aaggataacc cctttaagct tcacttgatt
     2041 gaggagaaag tgacaggtcc aacagcaaca gtatatgggt gtggcacatt ggttgacctt
     2101 tgccagggcc cccaccttcg gcatactgga cagattggag gactgaagct gctatcgaac
     2161 tcatcatcct tatggaggtc ttcaggggcc ccagagacac tgcagagagt gtcagggatt
     2221 tccttcccca caacagaatt gctgagggtc tgggaagcat ggagggagga agcagaattg
     2281 cgggaccacc ggcgcattgg gaaggaacag gagctcttct tcttccatga actgagccct
     2341 gggagctgct tcttcctgcc acgagggaca agggtgtata atgcactagt ggcgtttatc
     2401 agggctgagt atgcccatcg tggtttctcc gaggtgaaaa ctcccacact gttttctacg
     2461 aagctctggg aacagtcagg gcactgggag cattatcagg aagacatgtt tgccgtgcag
     2521 cccccaggct ctgacaggcc tcccagctcc cagagtgacg attctaccag gcatatcaca
     2581 gatacactcg ccctcaagcc tatgaactgc cctgcacact gcctgatgtt cgcccaccgg
     2641 cccagatcct ggcgggaact gcccctgcga ctagctgact ttggggctct acaccgggcc
     2701 gaagcctctg gtggtctggg gggactgacc cgactgcggt gcttccagca ggatgacgct
     2761 cacatcttct gtacaacaga tcagctggaa gcagagatcc aaagctgtct tgatttcctc
     2821 cgttccgtct atgccgttct tggcttctcc ttccgcctgg cactgtccac ccggccatct
     2881 ggcttcctgg gggacccttg cctttgggac caggccgaac aggtccttaa acaggccctg
     2941 aaggaatttg gagaaccctg ggacctcaac tctggagatg gtgccttcta tggacctaag
     3001 attgacgtgc acctccacga tgccctgggc cggccacatc agtgtgggac aattcagctt
     3061 gacttccaac tgcccctgag atttgacctc cagtataagg ggcaggcggg tgccctggag
     3121 cgtccagtcc tcattcaccg agcagtgctc ggttctgtgg aaagactgtt gggagtgctg
     3181 gcagaaagct gcggggggaa atggccactg tggctgtccc cgttccaggt ggtggtcatc
     3241 cctgtgggga gtgagcaaga ggaatacgcc aaagaggcac agcagagcct gcgggctgca
     3301 ggactggtca gtgacctgga tgcagactct ggactgaccc tcagccggag aatccgccgg
     3361 gcccagcttg cccactacaa ttttcagttt gtggttggcc agaaagagca aagtaagaga
     3421 acagtgaaca ttcggactcg agataatcgt cgccttgggg agtgggactt gcctgaggct
     3481 gtgcagcgac tggtggagct acagaacacg agggtcccaa atgccgaaga aattttctga
     3541 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     3601 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     3661 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3721 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3781 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3841 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3901 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3961 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4021 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4081 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4141 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4201 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     4261 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4321 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4381 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4441 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4501 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4561 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4621 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4681 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4741 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4801 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4861 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4921 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     4981 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5041 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5101 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5161 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5221 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5281 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     5341 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5401 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5461 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5521 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     5581 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     5641 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5701 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     5761 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     5821 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     5881 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     5941 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     6001 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     6061 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6121 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6181 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6241 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6301 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     6361 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6421 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6481 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6541 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6601 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6661 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6721 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6781 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6841 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
