LOCUS       V037158                 9658 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037158
VERSION     V037158
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1357..6282
                     /label="TRPM1(NM_001252020)"
                     /note="TRPM1(NM_001252020)"
                     /gene="TRPM1"
     polyA_signal    6446..6567
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(6574..7029)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7056..7160
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        7162..7519
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7554..8348
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8580..8627
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8956..9544
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 ggtggaggcg gatcaggagg cggtggatct ctcgagatga gctccttcaa gcggggctcg
     1381 ctcaagagct ccacatcagg gtcgcagaag ggtcagaaat cttggataga gaaaaccttt
     1441 tgcaaacggg aatgtatctt tgtaattcct agcatgaaag actctaacag gtgttgctgt
     1501 ggccagttca ccaaccagca tatcccccct ctgccaagtg caacacccag caaaaatgaa
     1561 gaggaaagca aacaggtgga gactcagcct gagaaatggt ctgttgccaa gcacacccag
     1621 agctacccaa cagattccta tggagttctt gaattccagg gtggcggata ttccaataaa
     1681 gccatgtata tccgtgtatc ctatgacacc aagccagact cactgctcca tctcatggtg
     1741 aaagattggc agctggaact ccccaagctc ttaatatctg tgcatggagg cctccagaac
     1801 tttgagatgc agcccaagct gaaacaagtc tttgggaaag gcctgatcaa ggctgctatg
     1861 accaccgggg cctggatctt caccgggggt gtcagcacag gtgttatcag ccacgtaggg
     1921 gatgccttga aagaccactc ctccaagtcc agaggccggg tttgtgctat aggaattgct
     1981 ccatggggca tcgtggagaa taaggaagac ctggttggaa aggatgtaac aagagtgtac
     2041 cagaccatgt ccaaccctct aagtaagctc tctgtgctca acaactccca cacccacttc
     2101 atcctggctg acaatggcac cctgggcaag tatggcgccg aggtgaagct gcgaaggctg
     2161 ctggaaaagc acatctccct gcagaagatc aacacaagac tggggcaggg cgtgcccctc
     2221 gtgggtctcg tggtggaggg gggccctaac gtggtgtcca tcgtcttgga atacctgcaa
     2281 gaagagcctc ccatccctgt ggtgatttgt gatggcagcg gacgtgcctc ggacatcctg
     2341 tcctttgcgc acaagtactg tgaagaaggc ggaataataa atgagtccct cagggagcag
     2401 cttctagtta ccattcagaa aacatttaat tataataagg cacaatcaca tcagctgttt
     2461 gcaattataa tggagtgcat gaagaagaaa gaactcgtca ctgtgttcag aatgggttct
     2521 gagggccagc aggacatcga gatggcaatt ttaactgccc tgctgaaagg aacaaacgta
     2581 tctgctccag atcagctgag cttggcactg gcttggaacc gcgtggacat agcacgaagc
     2641 cagatctttg tctttgggcc ccactggccg cccctgggaa gcctggcacc cccgacggac
     2701 agcaaagcca cggagaagga gaagaagcca cccatggcca ccaccaaggg aggaagagga
     2761 aaagggaaag gcaagaagaa agggaaagtg aaagaggaag tggaggaaga aactgacccc
     2821 cggaagatag agctgctgaa ctgggtgaat gctttggagc aagcgatgct agatgcttta
     2881 gtcttagatc gtgtcgactt tgtgaagctc ctgattgaaa acggagtgaa catgcaacac
     2941 tttctgacca ttccgaggct ggaggagctt tataacacaa gactgggtcc accaaacaca
     3001 cttcatctgc tggtgaggga tgtgaaaaag agcaaccttc cgcctgatta ccacatcagc
     3061 ctcatagaca tcgggctcgt gctggagtac ctcatgggag gagcctaccg ctgcaactac
     3121 actcggaaaa actttcggac cctttacaac aacttgtttg gaccaaagag gcctaaagct
     3181 cttaaacttc tgggaatgga agatgatgag cctccagcta aagggaagaa aaagaaaaag
     3241 aagaaaaagg aggaagagat cgacattgat gtggacgacc ctgccgtgag tcggttccag
     3301 tatcccttcc acgagctgat ggtgtgggca gtgctgatga aacgccagaa aatggcagtg
     3361 ttcctctggc agcgagggga agagagcatg gccaaggccc tggtggcctg caagctctac
     3421 aaggccatgg cccacgagtc ctccgagagt gatctggtgg atgacatctc ccaggacttg
     3481 gataacaatt ccaaagactt cggccagctt gctttggagt tattagacca gtcctataag
     3541 catgacgagc agatcgctat gaaactcctg acctacgagc tgaaaaactg gagcaactcg
     3601 acctgcctca aactggccgt ggcagccaaa caccgggact tcattgctca cacctgcagc
     3661 cagatgctgc tgaccgatat gtggatggga agactgcgga tgcggaagaa ccccggcctg
     3721 aaggttatca tggggattct tctacccccc accatcttgt ttttggaatt tcgcacatat
     3781 gatgatttct cgtatcaaac atccaaggaa aatgaggatg gcaaagaaaa agaagaggaa
     3841 aatacggatg caaatgcaga tgctggctca agaaaggggg atgaggagaa cgagcacaaa
     3901 aaacagagaa gtattcccat cggaacaaag atctgtgaat tctataacgc gcccattgtc
     3961 aagttctggt tttacacaat atcatacttg ggctacctgc tgctgtttaa ctacgtcatc
     4021 ctggtgcgga tggatggctg gccgtccctc caggagtgga tcgtcatctc ctacatcgtg
     4081 agcctggcgt tagagaagat acgagagatc ctcatgtcag aaccaggcaa actcagccag
     4141 aaaatcaaag tttggcttca ggagtactgg aacatcacag atctcgtggc catttccaca
     4201 ttcatgattg gagcaattct tcgcctacag aaccagccct acatgggcta tggccgggtg
     4261 atctactgtg tggatatcat cttctggtac atccgtgtcc tggacatctt tggtgtcaac
     4321 aagtatctgg ggccatacgt gatgatgatt ggaaagatga tgatcgacat gctgtacttt
     4381 gtggtcatca tgctggtcgt gctcatgagt ttcggagtag cccgtcaagc cattctgcat
     4441 ccagaggaga agccctcttg gaaactggcc cgaaacatct tctacatgcc ctactggatg
     4501 atctatggag aggtgtttgc agaccagata gacctctacg ccatggaaat taatcctcct
     4561 tgtggtgaga acctatatga tgaggagggc aagcggcttc ctccctgtat ccccggcgcc
     4621 tggctcactc cagcactcat ggcgtgctat ctactggtcg ccaacatcct gctggtgaac
     4681 ctgctgattg ctgtgttcaa caataccttc tttgaagtaa aatcaatatc caaccaggtg
     4741 tggaagttcc agcgatatca gctgattatg acatttcatg acaggccagt cctgccccca
     4801 ccgatgatca ttttaagcca catctacatc atcattatgc gtctcagcgg ccgctgcagg
     4861 aaaaagagag aaggggacca agaggaacgg gatcgtggat tgaagctctt ccttagcgac
     4921 gaggagctaa agaggctgca tgagttcgag gagcagtgcg tgcaggagca cttccgggag
     4981 aaggaggatg agcagcagtc gtccagcgac gagcgcatcc gggtcacttc tgaaagagtt
     5041 gaaaatatgt caatgaggtt ggaagaaatc aatgaaagag aaacttttat gaaaacttcc
     5101 ctgcagactg ttgaccttcg acttgctcag ctagaagaat tatctaacag aatggtgaat
     5161 gctcttgaaa atcttgcggg aatcgacagg tctgacctga tccaggcacg gtcccgggct
     5221 tcttctgaat gtgaggcaac gtatcttctc cggcaaagca gcatcaatag cgctgatggc
     5281 tacagcttgt atcgatatca ttttaacgga gaagagttat tatttgagga tacatctctc
     5341 tccacgtcac cagggacagg agtcaggaaa aaaacctgtt ccttccgtat aaaggaagag
     5401 aaggacgtga aaacgcacct agtcccagaa tgtcagaaca gtcttcacct ttcactgggc
     5461 acaagcacat cagcaacccc agatggcagt caccttgcag tagatgactt aaagaacgct
     5521 gaagagtcaa aattaggtcc agatattggg atttcaaagg aagatgatga aagacagaca
     5581 gactctaaaa aagaagaaac tatttcccca agtttaaata aaacagatgt gatacatgga
     5641 caggacaaat cagatgttca aaacactcag ctaacagtgg aaacgacaaa tatagaaggc
     5701 actatttcct atcccctgga agaaaccaaa attacacgct atttccccga tgaaacgatc
     5761 aatgcttgta aaacaatgaa gtccagaagc ttcgtctatt cccggggaag aaagctggtc
     5821 ggtggggtta accaggatgt agagtacagt tcaatcacgg accagcaatt gacgacggaa
     5881 tggcaatgcc aagttcaaaa gatcacgcgc tctcatagca cagatattcc ttacattgtg
     5941 tcggaagctg cagtgcaagc tgagcataaa gagcagtttg cagatatgca agatgaacac
     6001 catgtcgctg aagcaattcc tcgaatccct cgcttgtccc taaccattac tgacagaaat
     6061 gggatggaaa acttactgtc tgtgaagcca gatcaaactt tgggattccc atctctcagg
     6121 tcaaaaagtt tacatggaca tcctaggaat gtgaaatcca ttcagggaaa gttagacaga
     6181 tctggacatg ccagtagtgt aagcagctta gtaattgtgt ctggaatgac agcagaagaa
     6241 aaaaaggtta agaaagagaa agcttccaca gaaactgaat gctaggaatt ctgcagtcga
     6301 cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca gccataccac
     6361 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     6421 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     6481 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6541 tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa
     6601 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     6661 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     6721 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     6781 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     6841 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     6901 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     6961 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     7021 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7081 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7141 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     7201 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7261 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7321 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7381 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7441 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7501 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     7561 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     7621 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     7681 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     7741 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     7801 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     7861 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     7921 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     7981 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     8041 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     8101 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     8161 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     8221 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     8281 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     8341 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     8401 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     8461 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     8521 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     8581 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     8641 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     8701 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     8761 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     8821 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8881 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8941 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9001 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9061 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     9121 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9181 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9241 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9301 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9361 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9421 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9481 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9541 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9601 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
