LOCUS       pCMV-OPRM1(human)-HA    4987 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019084
VERSION     V019084
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     regulatory      891..900
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             901..2100
                     /label="OPRM1(NM_000914)"
                     /note="OPRM1(NM_000914)"
                     /gene="OPRM1"
     polyA_signal    2126..2260
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2371..2387)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2395..2411
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2419..2449)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2464..2485
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2773..3361)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3532..4392)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4393..4497)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     4971..4987
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
      841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcggtcgac gccaccatgg
      901 atggacagca gcgctgcccc cacgaacgcc agcaattgca ctgatgcctt ggcgtactca
      961 agttgctccc cagcacccag ccccggttcc tgggtcaact tgtcccactt agatggcaac
     1021 ctgtccgacc catgcggtcc gaaccgcacc gacctgggcg ggagagacag cctgtgccct
     1081 ccgaccggca gtccctccat gatcacggcc atcacgatca tggccctcta ctccatcgtg
     1141 tgcgtggtgg ggctcttcgg aaacttcctg gtcatgtatg tgattgtcag atacaccaag
     1201 atgaagactg ccaccaacat ctacattttc aaccttgctc tggcagatgc cttagccacc
     1261 agtaccctgc ccttccagag tgtgaattac ctaatgggaa catggccatt tggaaccatc
     1321 ctttgcaaga tagtgatctc catagattac tataacatgt tcaccagcat attcaccctc
     1381 tgcaccatga gtgttgatcg atacattgca gtctgccacc ctgtcaaggc cttagatttc
     1441 cgtactcccc gaaatgccaa aattatcaat gtctgcaact ggatcctctc ttcagccatt
     1501 ggtcttcctg taatgttcat ggctacaaca aaatacaggc aaggttccat agattgtaca
     1561 ctaacattct ctcatccaac ctggtactgg gaaaacctgc tgaagatctg tgttttcatc
     1621 ttcgccttca ttatgccagt gctcatcatt accgtgtgct atggactgat gatcttgcgc
     1681 ctcaagagtg tccgcatgct ctctggctcc aaagaaaagg acaggaatct tcgaaggatc
     1741 accaggatgg tgctggtggt ggtggctgtg ttcatcgtct gctggactcc cattcacatt
     1801 tacgtcatca ttaaagcctt ggttacaatc ccagaaacta cgttccagac tgtttcttgg
     1861 cacttctgca ttgctctagg ttacacaaac agctgcctca acccagtcct ttatgcattt
     1921 ctggatgaaa acttcaaacg atgcttcaga gagttctgta tcccaacctc ttccaacatt
     1981 gagcaacaaa actccactcg aattcgtcag aacactagag accacccctc cacggccaat
     2041 acagtggata gaactaatca tcagctagaa aatctggaag cagaaactgc tccgttgccc
     2101 taactcgagg taccgcggcc gcggggatcc agacatgata agatacattg atgagtttgg
     2161 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2221 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     2281 ttttatgttt caggttcagg gggaggtgtg ggaggttttt tcggatcctc tagagtcgat
     2341 ctgcaggcat gctagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2401 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2461 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2521 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2581 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2641 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2701 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3181 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3841 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4141 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4501 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     4561 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     4621 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     4681 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     4741 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     4801 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     4861 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     4921 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     4981 ggccagt
//
