LOCUS V035869 4325 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035869 VERSION V035869 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 27..330 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 331..534 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 672..768 /note="modified SV40 intron with splice donor and acceptor sites" /label="SV40 intron" CDS 844..1410 /label="PARK7(NM_007262)" /note="PARK7(NM_007262)" /gene="PARK7" CDS 1420..1449 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" polyA_signal 1464..1598 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(1709..1725) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 1733..1749 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(1757..1787) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 1802..1823 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(2111..2699) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(2870..3730) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(3731..3835) /gene="bla" /label="AmpR promoter" primer_bind 4309..4325 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc 61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta 541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac 601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag 661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg 721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa 781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcgcc 841 accatggctt ccaaaagagc tctggtcatc ctggctaaag gagcagagga aatggagacg 901 gtcatccctg tagatgtcat gaggcgagct gggattaagg tcaccgttgc aggcctggct 961 ggaaaagacc cagtacagtg tagccgtgat gtggtcattt gtcctgatgc cagccttgaa 1021 gatgcaaaaa aagagggacc atatgatgtg gtggttctac caggaggtaa tctgggcgca 1081 cagaatttat ctgagtctgc tgctgtgaag gagatactga aggagcagga aaaccggaag 1141 ggcctgatag ccgccatctg tgcaggtcct actgctctgt tggctcatga aataggtttt 1201 ggaagtaaag ttacaacaca ccctcttgct aaagacaaaa tgatgaatgg aggtcattac 1261 acctactctg agaatcgtgt ggaaaaagac ggcctgattc ttacaagccg ggggcctggg 1321 accagcttcg agtttgcgct tgcaattgtt gaagccctga atggcaagga ggtggcggct 1381 caagtgaagg ctccacttgt tcttaaagac ggtaccggcg agcagaagct gatctcagag 1441 gaggacctgt aagcggccgc ggggatccag acatgataag atacattgat gagtttggac 1501 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg 1561 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt 1621 ttatgtttca ggttcagggg gaggtgtggg aggttttttc ggatcctcta gagtcgatct 1681 gcaggcatgc tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 1741 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 1801 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 1861 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 1921 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 1981 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 2041 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 2101 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 2161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 2221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 2281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 2341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 2401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 2461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 2521 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 2581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 2641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 2701 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 2761 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 2821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 2881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 2941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 3001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 3061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 3121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 3181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 3241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 3301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 3361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 3421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 3481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 3541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 3601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 3661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 3721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 3781 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 3841 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 3901 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc 3961 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 4021 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 4081 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 4141 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 4201 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 4261 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 4321 ccagt //