LOCUS       V043001                 8167 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043001
VERSION     V043001
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             635..3217
                     /label="Pde6c(NM_033614)"
                     /note="Pde6c(NM_033614)"
                     /gene="Pde6c"
     CDS             3230..3295
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3336..3560
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     promoter        3576..4099
                     /label="mCMV promoter"
     CDS             4113..4832
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4955..5076
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5083..5538)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5565..5669
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5671..6028
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6063..6857
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7089..7136
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7465..8053
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct ccgaattcgc caccatgggt gagatcagcc aagaggccgt
      661 ggagcgctac ctggagaaga acccttgttt tgccaaagag tattttgaca agaaactacg
      721 ggtggaggca ctgggggtga tcttcaaaaa cagccatgca ggggtccaga ccggcttgtc
      781 gctccctgag atgacacagg tggaggagtc ggccgtgtgc ctggagctac tgcagtgtat
      841 gcaggacgag gccggaagcg cggagcagat ggctcaccgg gcactgcaga ggctcgctca
      901 acttctgcag gctgattgct gcagtatgtt ctcgtgcaga gcaagaaacg gcataccaga
      961 ggtggcctcc aggctgctga acgtcacccc aacttctaag tttgaggaca acctcgtggc
     1021 ccctgacaga gaagttgtat ttccgttgga cattgggata gttggctggg tcgctcatgt
     1081 gaagaaagcc cttaatgtct cagatgtcaa aaagaacagc catttttctg actttatgga
     1141 caagcaaact ggatatgtca ccaggaatct gctagcagtc ccgatcgtgg caggcaagga
     1201 ggtccttgct gtggtcatgg cagtgaacaa aatcagcgct cccgagtttt ccaaacagga
     1261 tgaggaggtc ttttctaagt acctcagctt tgttgctgtc gcactaaggc tgcagcacac
     1321 cagttacctg tacagtgtgg agtcccggag aagccagatc cttatgtggt cagccaataa
     1381 agtattcgaa gagctcactg atgttgagcg acagtttcac aaggcactgt acaccatcag
     1441 gacgtacctg aactgcgatc gctactccat tggtcttctg gacatgacca aggagaagga
     1501 attttacgat gagtggccaa tcaagcttgg agaagttgag ccttataaag gaccaaagac
     1561 tccagatggc agggaaatca tcttctataa aatcatcgat tacattttac atggaaaaga
     1621 agagatcaac gtgattccgt cacctccagc agaccactgg acgcttgtta gtggattgcc
     1681 aacatacgtt gcagaaaatg gatttatctg taacatgctg aatgcccctg cagatgagta
     1741 cttcacattt cagaaaggac cggtagatga gactggctgg gtcattaaaa atgtcttgtc
     1801 cctgcctatt gtcaacaaga aagaagacat cgtgggtgta gctacgtttt acaaccggaa
     1861 ggatgggaaa ccctttgatg agcatgatga acatatcact gagactttaa cccagtttct
     1921 tggatggtct cttttaaaca ccgataccta tgagcgagtg aacaagctgg aaagcagaaa
     1981 ggacattgct caggaaatgg ttatgaacct cacaaaagcc accccggatg aaatcagctc
     2041 tattctgaag tttaaagaga agttaaatgt agaggtaatt gaagagtgtg aagagagaca
     2101 gctcctggcc atattgaagg aggacttgcc ggacccgaga acagcagacc tgtacgagtt
     2161 ctgtttcagc gacttcccca ttacggagca cgagttggtt aaatgcgggc tgaggctgtt
     2221 tttggagata aacgtggtgg agaaattcaa agtccctgta gaggttctta caaggtggat
     2281 gtacacagtc cggaaagggt accgtcctgt cacctaccac aactggcggc acggattcaa
     2341 cgtggggcag accatgttca ctttgttaat gacaggaaga ctaaagaaat actatacaga
     2401 ccttgaagcc tttgccatgc tcgctgctgc tttctgccat gacattgacc acagaggcac
     2461 caataacttg taccaaatga agtccacatc tccacttgca aggcttcatg ggacttccat
     2521 tttagagcga catcacctgg aatatagcaa gactctcttg caggatgaga gtttaaacat
     2581 cttccagaat ctaaacaagc ggcagtttga aacggtgatc catttgttcg aggttgcaat
     2641 aatagcaact gacctggcac tctatttcaa gaagagaacc atgtttcaaa agatcgttga
     2701 tacctgcgag cagatgcaaa gtgaagaaga aaccatcaaa tacgtaacct ccgacccgac
     2761 caaaaaggaa gtcatcatgg ccatgatgat gactgcctgt gacctgtctg ccatcaccaa
     2821 gccctgggag gtgcagagtc aggtagcact tctggttgca aatgaatttt gggaacaagg
     2881 agatctggaa agaacagtgc tgcagcagca accaattcct atgatggaca gaagcaaaaa
     2941 ggatgaactc ccgaaacttc aagtgggatt tattgatttt gtttgtactt tcgtatataa
     3001 ggagttctct cggtttcatg gagagatcac acccatgctg aacggccttc agaataacag
     3061 ggtggaatgg aaatccctgg cggaggagta cgaggcgaag gtgaaggtga ctgaagaaga
     3121 ggcgggaaag caggaagaag aagcctcaga tggaaaagct gccacagatt taggaggcag
     3181 cgccgaagac aaaaagtcca aaacatgctt aatgttaaag cttacgcgtg actacaaaga
     3241 ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg acaagtgagg
     3301 atccgggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     3361 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     3421 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     3481 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     3541 ggatgcggtg ggctctatgg tctagagata tactgagtca ttagggactt tccaatgggt
     3601 tttgcccagt acataaggtc aataggggtg aatcaacagg aaagtcccat tggagccaag
     3661 tacactgagt caatagggac tttccattgg gttttgccca gtacaaaagg tcaatagggg
     3721 gtgagtcaat gggtttttcc cattattggc acgtacataa ggtcaatagg ggtgagtcat
     3781 tgggtttttc cagccaattt aattaaaacg ccatgtactt tcccaccatt gacgtcaatg
     3841 ggctattgaa actaatgcaa cgtgaccttt aaacggtact ttcccatagc tgattaatgg
     3901 gaaagtaccg ttctcgagcc aatacacgtc aatgggaagt gaaagggcag ccaaaacgta
     3961 acaccgcccc ggttttcccc tggaaattcc atattggcac gcattctatt ggctgagctg
     4021 cgttctacgt gggtataaga ggcgcgacca gcgtcggtac cgtcgcagtc ttcggtctga
     4081 ccaccgtaga acgcagatca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     4141 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4201 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     4261 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     4321 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4381 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4441 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4501 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4561 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4621 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4681 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     4741 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4801 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag
     4861 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     4921 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     4981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5041 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     5101 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     5161 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     5221 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     5281 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     5341 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     5401 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     5461 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     5521 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5641 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     5701 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5761 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     5821 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5881 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5941 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6001 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     6061 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     6121 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     6181 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     6241 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     6301 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     6361 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     6421 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     6481 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     6541 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     6601 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     6661 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     6721 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     6781 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     6841 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     6901 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     6961 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7021 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     7081 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     7141 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     7201 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     7261 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     7321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     7621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8161 catgcat
//
