LOCUS       V025252                 7039 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025252
VERSION     V025252
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..2667
                     /label="IGF2BP3(NM_006547)"
                     /note="IGF2BP3(NM_006547)"
                     /gene="IGF2BP3"
     CDS             2674..2700
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2704..2730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2734..2760
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    2801..3025
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3071..3499
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3513..3842
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3909..4508
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    4682..4803
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(4852..4868)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4876..4892
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4900..4930)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4945..4966
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5254..5839)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6010..6870)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6871..6975)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgaacaaac tgtatatcgg aaacctcagc
      961 gagaacgccg ccccctcgga cctagaaagt atcttcaagg acgccaagat cccggtgtcg
     1021 ggacccttcc tggtgaagac tggctacgcg ttcgtggact gcccggacga gagctgggcc
     1081 ctcaaggcca tcgaggcgct ttcaggtaaa atagaactgc acgggaaacc catagaagtt
     1141 gagcactcgg tcccaaaaag gcaaaggatt cggaaacttc agatacgaaa tatcccgcct
     1201 catttacagt gggaggtgct ggatagttta ctagtccagt atggagtggt ggagagctgt
     1261 gagcaagtga acactgactc ggaaactgca gttgtaaatg taacctattc cagtaaggac
     1321 caagctagac aagcactaga caaactgaat ggatttcagt tagagaattt caccttgaaa
     1381 gtagcctata tccctgatga aatggccgcc cagcaaaacc ccttgcagca gccccgaggt
     1441 cgccgggggc ttgggcagag gggctcctca aggcaggggt ctccaggatc cgtatccaag
     1501 cagaaaccat gtgatttgcc tctgcgcctg ctggttccca cccaatttgt tggagccatc
     1561 ataggaaaag aaggtgccac cattcggaac atcaccaaac agacccagtc taaaatcgat
     1621 gtccaccgta aagaaaatgc gggggctgct gagaagtcga ttactatcct ctctactcct
     1681 gaaggcacct ctgcggcttg taagtctatt ctggagatta tgcataagga agctcaagat
     1741 ataaaattca cagaagagat ccccttgaag attttagctc ataataactt tgttggacgt
     1801 cttattggta aagaaggaag aaatcttaaa aaaattgagc aagacacaga cactaaaatc
     1861 acgatatctc cattgcagga attgacgctg tataatccag aacgcactat tacagttaaa
     1921 ggcaatgttg agacatgtgc caaagctgag gaggagatca tgaagaaaat cagggagtct
     1981 tatgaaaatg atattgcttc tatgaatctt caagcacatt taattcctgg attaaatctg
     2041 aacgccttgg gtctgttccc acccacttca gggatgccac ctcccacctc agggccccct
     2101 tcagccatga ctcctcccta cccgcagttt gagcaatcag aaacggagac tgttcatctg
     2161 tttatcccag ctctatcagt cggtgccatc atcggcaagc agggccagca catcaagcag
     2221 ctttctcgct ttgctggagc ttcaattaag attgctccag cggaagcacc agatgctaaa
     2281 gtgaggatgg tgattatcac tggaccacca gaggctcagt tcaaggctca gggaagaatt
     2341 tatggaaaaa ttaaagaaga aaactttgtt agtcctaaag aagaggtgaa acttgaagct
     2401 catatcagag tgccatcctt tgctgctggc agagttattg gaaaaggagg caaaacggtg
     2461 aatgaacttc agaatttgtc aagtgcagaa gttgttgtcc ctcgtgacca gacacctgat
     2521 gagaatgacc aagtggttgt caaaataact ggtcacttct atgcttgcca ggttgcccag
     2581 agaaaaattc aggaaattct gactcaggta aagcagcacc aacaacagaa ggctctgcaa
     2641 agtggaccac ctcagtcaag acggaagctc gagtacccat acgacgtacc tgattatgca
     2701 acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca
     2761 tagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     2821 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     2881 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     2941 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     3001 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     3061 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3121 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3181 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3241 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3301 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3361 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3421 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3481 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3541 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3601 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3661 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3721 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3781 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3841 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     3901 cgtttcgcat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     3961 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     4021 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     4081 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     4141 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     4201 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     4261 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     4321 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     4381 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     4441 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     4501 gtgcctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     4561 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     4621 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     4681 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4741 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4801 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     4861 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     4921 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4981 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5041 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5101 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5161 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5221 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5281 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5341 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5401 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5461 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5521 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5581 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5641 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5701 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5761 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg
     5821 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5881 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5941 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6001 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6061 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6121 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6181 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6241 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6301 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6361 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6421 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6481 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6541 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6601 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6661 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6721 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6781 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6841 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6901 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6961 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     7021 tctcccgatc ccctatggt
//
