LOCUS       V040042                10919 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040042
VERSION     V040042
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..5878
                     /label="IQGAP1(NM_003870)"
                     /note="IQGAP1(NM_003870)"
                     /gene="IQGAP1"
     CDS             5927..6637
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    6681..6905
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6951..7379
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7393..7722
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7789..8388
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    8562..8683
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8732..8748)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8756..8772
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8780..8810)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8825..8846
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9134..9719)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9890..10750)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10751..10855)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg tccgccgcag acgaggttga cgggctgggc gtggcccggc cgcactatgg
      961 ctctgtcctg gataatgaaa gacttactgc agaggagatg gatgaaagga gacgtcagaa
     1021 cgtggcttat gagtaccttt gtcatttgga agaagcgaag aggtggatgg aagcatgcct
     1081 aggggaagat ctgcctccca ccacagaact ggaggagggg cttaggaatg gggtctacct
     1141 tgccaaactg gggaacttct tctctcccaa agtagtgtcc ctgaaaaaaa tctatgatcg
     1201 agaacagacc agatacaagg cgactggcct ccactttaga cacactgata atgtgattca
     1261 gtggttgaat gccatggatg agattggatt gcctaagatt ttttacccag aaactacaga
     1321 tatctatgat cgaaagaaca tgccaagatg tatctactgt atccatgcac tcagtttgta
     1381 cctgttcaag ctaggcctgg cccctcagat tcaagaccta tatggaaagg ttgacttcac
     1441 agaagaagaa atcaacaaca tgaagactga gttggagaag tatggcatcc agatgcctgc
     1501 ctttagcaag attgggggca tcttggctaa tgaactgtca gtggatgaag ccgcattaca
     1561 tgctgctgtt attgctatta atgaagctat tgaccgtaga attccagccg acacatttgc
     1621 agctttgaaa aatccgaatg ccatgcttgt aaatcttgaa gagcccttgg catccactta
     1681 ccaggatata ctttaccagg ctaagcagga caaaatgaca aatgctaaaa acaggacaga
     1741 aaactcagag agagaaagag atgtttatga ggagctgctc acgcaagctg aaattcaagg
     1801 caatataaac aaagtcaata cattttctgc attagcaaat atcgacctgg ctttagaaca
     1861 aggagatgca ctggccttgt tcagggctct gcagtcacca gccctggggc ttcgaggact
     1921 gcagcaacag aatagcgact ggtacttgaa gcagctcctg agtgataaac agcagaagag
     1981 acagagtggt cagactgacc ccctgcagaa ggaggagctg cagtctggag tggatgctgc
     2041 aaacagtgct gcccagcaat atcagagaag attggcagca gtagcactga ttaatgctgc
     2101 aatccagaag ggtgttgctg agaagactgt tttggaactg atgaatcccg aagcccagct
     2161 gccccaggtg tatccatttg ccgccgatct ctatcagaag gagctggcta ccctgcagcg
     2221 acaaagtcct gaacataatc tcacccaccc agagctctct gtcgcagtgg agatgttgtc
     2281 atcggtggcc ctgatcaaca gggcattgga atcaggagat gtgaatacag tgtggaagca
     2341 attgagcagt tcagttactg gtcttaccaa tattgaggaa gaaaactgtc agaggtatct
     2401 cgatgagttg atgaaactga aggctcaggc acatgcagag aataatgaat tcattacatg
     2461 gaatgatatc caagcttgcg tggaccatgt gaacctggtg gtgcaagagg aacatgagag
     2521 gattttagcc attggtttaa ttaatgaagc cctggatgaa ggtgatgccc aaaagactct
     2581 gcaggcccta cagattcctg cagctaaact tgagggagtc cttgcagaag tggcccagca
     2641 ttaccaagac acgctgatta gagcgaagag agagaaagcc caggaaatcc aggatgagtc
     2701 agctgtgtta tggttggatg aaattcaagg tggaatctgg cagtccaaca aagacaccca
     2761 agaagcacag aagtttgcct taggaatctt tgccattaat gaggcagtag aaagtggtga
     2821 tgttggcaaa acactgagtg cccttcgctc ccctgatgtt ggcttgtatg gagtcatccc
     2881 tgagtgtggt gaaacttacc acagtgatct tgctgaagcc aagaagaaaa aactggcagt
     2941 aggagataat aacagcaagt gggtgaagca ctgggtaaaa ggtggatatt attattacca
     3001 caatctggag acccaggaag gaggatggga tgaacctcca aattttgtgc aaaattctat
     3061 gcagctttct cgggaggaga tccagagttc tatctctggg gtgactgccg catataaccg
     3121 agaacagctg tggctggcca atgaaggcct gatcaccagg ctgcaggctc gctgccgtgg
     3181 atacttagtt cgacaggaat tccgatccag gatgaatttc ctgaagaaac aaatccctgc
     3241 catcacctgc attcagtcac agtggagagg atacaagcag aagaaggcat atcaagatcg
     3301 gttagcttac ctgcgctccc acaaagatga agttgtaaag attcagtccc tggcaaggat
     3361 gcaccaagct cgaaagcgct atcgagatcg cctgcagtac ttccgggacc atataaatga
     3421 cattatcaaa atccaggctt ttattcgggc aaacaaagct cgggatgact acaagactct
     3481 catcaatgct gaggatcctc ctatggttgt ggtccgaaaa tttgtccacc tgctggacca
     3541 aagtgaccag gattttcagg aggagcttga ccttatgaag atgcgggaag aggttatcac
     3601 cctcattcgt tctaaccagc agctggagaa tgacctcaat ctcatggata tcaaaattgg
     3661 actgctagtg aaaaataaga ttacgttgca ggatgtggtt tcccacagta aaaaacttac
     3721 caaaaaaaat aaggaacagt tgtctgatat gatgatgata aataaacaga agggaggtct
     3781 caaggctttg agcaaggaga agagagagaa gttggaagct taccagcacc tgttttattt
     3841 attgcaaacc aatcccacct atctggccaa gctcattttt cagatgcccc agaacaagtc
     3901 caccaagttc atggactctg taatcttcac actctacaac tacgcgtcca accagcgaga
     3961 ggagtacctg ctcctgcggc tctttaagac agcactccaa gaggaaatca agtcgaaggt
     4021 agatcagatt caagagattg tgacaggaaa tcctacggtt attaaaatgg ttgtaagttt
     4081 caaccgtggt gcccgtggcc agaatgccct gagacagatc ttggccccag tcgtgaagga
     4141 aattatggat gacaaatctc tcaacatcaa aactgaccct gtggatattt acaaatcttg
     4201 ggttaatcag atggagtctc agacaggaga ggcaagcaaa ctgccctatg atgtgacccc
     4261 tgagcaggcg ctagctcatg aagaagtgaa gacacggcta gacagctcca tcaggaacat
     4321 gcgggctgtg acagacaagt ttctctcagc cattgtcagc tctgtggaca aaatccctta
     4381 tgggatgcgc ttcattgcca aagtgctgaa ggactcgttg catgagaagt tccctgatgc
     4441 tggtgaggat gagctgctga agattattgg taacttgctt tattatcgat acatgaatcc
     4501 agccattgtt gctcctgatg cctttgacat cattgacctg tcagcaggag gccagcttac
     4561 cacagaccaa cgccgaaatc tgggctccat tgcaaaaatg cttcagcatg ctgcttccaa
     4621 taagatgttt ctgggagata atgcccactt aagcatcatt aatgaatatc tttcccagtc
     4681 ctaccagaaa ttcagacggt ttttccaaac tgcttgtgat gtcccagagc ttcaggataa
     4741 atttaatgtg gatgagtact ctgatttagt aaccctcacc aaaccagtaa tctacatttc
     4801 cattggtgaa atcatcaaca cccacactct cctgttggat caccaggatg ccattgctcc
     4861 ggagcacaat gatccaatcc acgaactgct ggacgacctc ggcgaggtgc ccaccatcga
     4921 gtccctgata ggggaaagct ctggcaattt aaatgaccca aataaggagg cactggctaa
     4981 gacggaagtg tctctcaccc tgaccaacaa gttcgacgtg cctggagatg agaatgcaga
     5041 aatggatgct cgaaccatct tactgaatac aaaacgttta attgtggatg tcatccggtt
     5101 ccagccagga gagaccttga ctgaaatcct agaaacacca gccaccagtg aacaggaagc
     5161 agaacatcag agagccatgc agagacgtgc tatccgtgat gccaaaacac ctgacaagat
     5221 gaaaaagtca aaatctgtaa aggaagacag caacctcact cttcaagaga agaaagagaa
     5281 gatccagaca ggtttaaaga agctaacaga gcttggaacc gtggacccaa agaacaaata
     5341 ccaggaactg atcaacgaca ttgccaggga tattcggaat cagcggaggt accgacagag
     5401 gagaaaggcc gaactagtga aactgcaaca gacatacgct gctctgaact ctaaggccac
     5461 cttttatggg gagcaggtgg attactataa aagctatatc aaaacctgct tggataactt
     5521 agccagcaag ggcaaagtct ccaaaaagcc tagggaaatg aaaggaaaga aaagcaaaaa
     5581 gatttctctg aaatatacag cagcaagact acatgaaaaa ggagttcttc tggaaattga
     5641 ggacctgcaa gtgaatcagt ttaaaaatgt tatatttgaa atcagtccaa cagaagaagt
     5701 tggagacttc gaagtgaaag ccaaattcat gggagttcaa atggagactt ttatgttaca
     5761 ttatcaggac ctgctgcagc tacagtatga aggagttgca gtcatgaaat tatttgatag
     5821 agctaaagta aatgtcaacc tcctgatctt ccttctcaac aaaaagttct acgggaagga
     5881 attcgtcatg gatatcggtg gaggcggatc aggaggcggt ggatctatgg tgagcaaggg
     5941 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     6001 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     6061 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     6121 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     6181 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     6241 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     6301 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     6361 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     6421 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     6481 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     6541 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     6601 ccactccacc ggcggcatgg acgagctgta caagtagctc gagtctagag ggcccgttta
     6661 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6721 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6781 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     6841 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     6901 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     6961 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7021 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7081 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7141 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7201 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7261 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7321 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     7381 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7441 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7501 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7561 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7621 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     7681 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     7741 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gaccgagtac
     7801 aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc
     7861 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag
     7921 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     7981 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     8041 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     8101 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     8161 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     8221 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     8281 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     8341 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc gggactctgg
     8401 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     8461 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     8521 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     8581 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8641 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     8701 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8761 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8821 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8881 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8941 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9001 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9061 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9181 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9241 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9301 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9361 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9421 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9481 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9541 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9601 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9661 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9721 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9781 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9841 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9901 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9961 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10021 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10081 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10141 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10201 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10261 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10321 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10381 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10441 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10501 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10561 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10621 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10681 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10741 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10801 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10861 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
