LOCUS V039768 7790 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039768 VERSION V039768 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..2749 /label="NCOA4(NM_005437)" /note="NCOA4(NM_005437)" /gene="NCOA4" CDS 2798..3508 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" polyA_signal 3552..3776 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3822..4250 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4264..4593 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4660..5259 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 5433..5554 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5603..5619) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5627..5643 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5651..5681) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5696..5717 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6005..6590) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6761..7621) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7622..7726) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg aataccttcc aagaccagag tggcagctcc agtaatagag aacccctttt 961 gaggtgtagt gatgcacgga gggacttgga gcttgctatt ggtggagttc tccgggctga 1021 acagcaaatt aaagataact tgcgagaggt caaagctcag attcacagtt gcataagccg 1081 tcacctggaa tgtcttagaa gccgtgaggt atggctgtat gaacaggtgg accttattta 1141 tcagcttaaa gaggagacac ttcaacagca ggctcagcag ctctactcgt tattgggcca 1201 gttcaattgt cttactcatc aactggagtg tacccaaaac aaagatctag ccaatcaagt 1261 ctctgtgtgc ctggagagac tgggcagttt gacccttaag cctgaagatt caactgtcct 1321 gctctttgaa gctgacacaa ttactctgcg ccagaccatc accacatttg ggtctctcaa 1381 aaccattcaa attcctgagc acttgatggc tcatgctagt tcagcaaata ttgggccctt 1441 cctggagaag agaggctgta tctccatgcc agagcagaag tcagcatccg gtattgtagc 1501 tgtccctttc agcgaatggc tccttggaag caaacctgcc agtggttatc aagctcctta 1561 catacccagc accgaccccc aggactggct tacccaaaag cagaccttgg agaacagtca 1621 gacttcttcc agagcctgca atttcttcaa taatgtcggg ggaaacctaa agggcttaga 1681 aaactggctc ctcaagagtg aaaaatcaag ttatcaaaag tgtaacagcc attccactac 1741 tagttctttc tccattgaaa tggaaaaggt tggagatcaa gagcttcctg atcaagatga 1801 gatggaccta tcagattggc tagtgactcc ccaggaatcc cataagctgc ggaagcctga 1861 gaatggcagt cgtgaaacca gtgagaagtt taagctctta ttccagtcct ataatgtgaa 1921 tgattggctt gtcaagactg actcctgtac caactgtcag ggaaaccagc ccaaaggtgt 1981 ggagattgaa aacctgggca atctgaagtg cctgaatgac cacttggagg ccaagaaacc 2041 attgtccacc cccagcatgg ttacagagga ttggcttgtc cagaaccatc aggacccatg 2101 taaggtagag gaggtgtgca gagccaatga gccctgcaca agctttgcag agtgtgtgtg 2161 tgatgagaat tgtgagaagg aggctctgta taagtggctt ctgaagaaag aaggaaagga 2221 taaaaatggg atgcctgtgg aacccaaacc tgagcctgag aagcataaag attccctgaa 2281 tatgtggctc tgtcctagaa aagaagtaat agaacaaact aaagcaccaa aggcaatgac 2341 tccttctaga attgctgatt ccttccaagt cataaagaac agccccttgt cggagtggct 2401 tatcaggccc ccatacaaag aaggaagtcc caaggaagtg cctggtactg aagacagagc 2461 tggcaaacag aagtttaaaa gccccatgaa tacttcctgg tgttccttta acacagctga 2521 ctgggtcctg ccaggaaaga agatgggcaa cctcagccag ttatcttctg gagaagacaa 2581 gtggctgctt cgaaagaagg cccaggaagt attacttaat tcacctctac aggaggaaca 2641 taacttcccc ccagaccatt atggcctccc tgcagtttgt gatctctttg cctgtatgca 2701 gcttaaagtt gataaagaga agtggttata tcgaactcct ctacagatgg aattcgtcat 2761 ggatatcggt ggaggcggat caggaggcgg tggatctatg gtgagcaagg gcgaggagga 2821 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa 2881 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac 2941 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc 3001 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt 3061 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg 3121 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa 3181 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg 3241 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca 3301 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc 3361 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc 3421 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac 3481 cggcggcatg gacgagctgt acaagtagct cgagtctaga gggcccgttt aaacccgctg 3541 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 3601 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 3661 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 3721 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 3781 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 3841 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 3901 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 3961 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 4021 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 4081 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 4141 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 4201 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 4261 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 4321 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 4381 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 4441 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 4501 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga 4561 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt 4621 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgaccgagta caagcccacg 4681 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc 4741 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc 4801 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 4861 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 4921 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 4981 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 5041 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 5101 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 5161 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 5221 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag cgggactctg gggttcgaaa 5281 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 5341 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 5401 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 5461 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 5521 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 5581 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 5641 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 5701 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 5761 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 5821 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 5881 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 5941 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 6001 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 6061 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 6121 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 6181 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 6241 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 6301 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 6361 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 6421 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 6481 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 6541 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 6601 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 6661 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 6721 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 6781 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 6841 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 6901 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 6961 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 7021 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 7081 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 7141 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 7201 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 7261 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 7321 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 7381 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 7441 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 7501 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 7561 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 7621 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 7681 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 7741 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt //