LOCUS 40924_11740 6575 bp DNA circular SYN 01-JAN-1980 DEFINITION Control vector for constitutive high-level expression of intracellular red firefly luciferase under control of the CMV promoter. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6575) AUTHORS Thermo Scientific (Pierce) TITLE Direct Submission REFERENCE 2 (bases 1 to 6575) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6575 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 64..367 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 368..571 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 616..634 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 647..2290 /codon_start=1 /label=Red Firefly Luciferase /note="intracellular red firefly luciferase" /translation="MENMENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFT NAVTGVDYSYAEYLEKSCCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGV APTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGY QCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFS HARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQD YKCTYVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQ GYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPML MKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELES VLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGG VRFVDEVPKGLTGKIDGRAIREILKKPVAKM" polyA_signal 2319..2430 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 2553..2882 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 2930..2977 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 2996..3592 /codon_start=1 /label=PuroR /note="puromycin N-acetyltransferase" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 3725..3858 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(3902..4759) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5023..5611 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5881..5897 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 6353..6369 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 6484..6575 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" ORIGIN 1 aactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 361 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg 421 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca 481 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg 541 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta 601 ctggcttatc gaaattaata cgactcacta taggggatcc gccaccatgg aaaatatgga 661 aaacgacgag aacatcgtgg tgggccccaa gcccttctac cccatcgagg aaggcagcgc 721 cggcacccag ctgcggaagt acatggaaag atacgccaag ctgggcgcca ttgccttcac 781 caacgccgtg accggcgtgg actacagcta cgccgagtac ctggaaaaga gctgctgcct 841 gggcaaggct ctgcagaact acggcctggt ggtggacggc cggatcgccc tgtgcagcga 901 gaactgcgag gaattcttca tccccgtgat cgccggcctg ttcatcggcg tgggcgtggc 961 tcccaccaac gagatctaca ccctgcggga gctggtgcac agcctgggca tcagcaagcc 1021 caccatcgtg ttcagcagca agaagggcct ggacaaagtc atcaccgtgc agaaaaccgt 1081 gaccaccatc aagaccatcg tgatcctgga cagcaaggtg gactaccggg gctaccagtg 1141 cctggacacc ttcatcaagc ggaacacccc ccctggcttc caggccagca gcttcaagac 1201 cgtggaggtg gaccggaaag aacaggtggc cctgatcatg aacagcagcg gcagcaccgg 1261 cctgcccaag ggcgtgcagc tgacccacga gaacaccgtg acccggttca gccacgccag 1321 ggaccccatc tacggcaacc aggtgtcccc cggcaccgcc gtgctgaccg tggtgccctt 1381 ccaccacggc ttcggcatgt tcaccaccct gggctacctg atctgcggct tccgggtggt 1441 gatgctgacc aagttcgacg aggaaacctt cctgaaaacc ctgcaggact acaagtgcac 1501 ctacgtgatt ctggtgccca ccctgttcgc catcctgaac aagagcgagc tgctgaacaa 1561 gtacgacctg agcaacctgg tggagatcgc cagcggcgga gcccccctga gcaaagaagt 1621 gggagaggcc gtcgccaggc ggttcaatct gcccggcgtg cggcagggct acggcctgac 1681 cgagacaacc agcgccatca tcatcacccc cgagggcgac gacaagcctg gagccagcgg 1741 caaggtggtg cccctgttca aggccaaagt gatcgacctg gacaccaaga agagcctggg 1801 ccccaacaga cggggcgaag tgtgcgtgaa gggccccatg ctgatgaagg gctacgtgaa 1861 caaccccgag gccaccaaag agctgatcga cgaagagggc tggctgcaca ccggcgacat 1921 cggctactac gacgaagaga agcacttctt catcgtggac cggctgaaga gcctgatcaa 1981 gtacaagggc tatcaggtgc cccctgccga gctggaaagc gtcctgctgc agcaccccag 2041 catcttcgac gccggcgtgg ccggggtgcc agatcctgtg gccggcgagc tgcctggcgc 2101 cgtggtggtg ctggaatccg gcaagaacat gaccgagaaa gaagtgatgg actacgtcgc 2161 cagccaggtg tccaacgcca agcggctgag aggcggcgtg agattcgtgg acgaagtgcc 2221 aaagggcctg accggcaaga tcgacggcag ggccatccgg gagatcctga agaaacccgt 2281 ggccaagatg tgatgagcgg ccgcaaaatc agcctcgact gtgccttcta gttgccagcc 2341 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 2401 cctttcctaa taaaatgagg aaattgcatc acaacactca accctatctc ggtctattct 2461 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 2521 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 2581 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 2641 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2701 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 2761 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 2821 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 2881 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta 2941 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatgac 3001 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac 3061 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca 3121 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 3181 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 3241 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 3301 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 3361 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 3421 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 3481 atccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 3541 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacacgt 3601 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 3661 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 3721 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 3781 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 3841 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcatt 3901 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 3961 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 4021 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 4081 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 4141 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 4201 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 4261 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 4321 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 4381 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 4441 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 4501 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 4561 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 4621 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 4681 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 4741 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 4801 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 4861 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 4921 gctcataaca ccccttgctc atgaccaaaa tcccttaacg tgagttacgc gcgcgtcgtt 4981 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 5041 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 5101 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 5161 aaatactgtt cttctagtgt agccgtagtt agcccaccac ttcaagaact ctgtagcacc 5221 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 5281 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 5341 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 5401 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 5461 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 5521 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 5581 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 5641 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 5701 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 5761 gcgcagcgag tcagtgagcg aggaagcgga aggcgagagt agggaactgc caggcatcaa 5821 actaagcaga aggcccctga cggatggcct ttttgcgttt ctacaaactc tttctgtgtt 5881 gtaaaacgac ggccagtctt aagctcgggc cccctgggcg gttctgataa cgagtaatcg 5941 ttaatccgca aataacgtaa aaacccgctt cggcgggttt ttttatgggg ggagtttagg 6001 gaaagagcat ttgtcagaat atttaagggc gcctgtcact ttgcttgata tatgagaatt 6061 atttaacctt ataaatgaga aaaaagcaac gcactttaaa taagatacgt tgctttttcg 6121 attgatgaac acctataatt aaactattca tctattattt atgatttttt gtatatacaa 6181 tatttctagt ttgttaaaga gaattaagaa aataaatctc gaaaataata aagggaaaat 6241 cagtttttga tatcaaaatt atacatgtca acgataatac aaaatataat acaaactata 6301 agatgttatc agtatttatt atcatttaga ataaattttg tgtcgccctt aattgtgagc 6361 ggataacaat tacgagcttc atgcacagtg gcgttgacat tgattattga ctagcatgtt 6421 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt 6481 aataacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt 6541 ccccagtgca agtgcaggtg ccagaacatt tctct //