LOCUS       pCMV_SPORT2.        4474 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for cloning and transient mammalian cell expression of cDNAs.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV SPORT2.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(125..259)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     664..680
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        691..709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    727..824
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(832..850)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(873..889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1015..1218)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1219..1522)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(1552..1568)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1576..1607)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     rep_origin      complement(2069..2657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    complement(2735..2768)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2873..3730)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(4018..4473)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
      301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
      361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct
      421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact
      481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta
      541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
      601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagatctcc cagtcacgac
      661 gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg agagagctat
      721 gacgtcgcat gcacgcgtaa gcttgggccc ctcgagggat cctctagagc ggccgccgac
      781 tagtgagctc gtcgacccgg gaattccgga ccggtacctg caggcgtacc ttctatagtg
      841 tcacctaaat agctttttgc aaaagcctag tggtcatagc tgtttcctgt gtgaaattgt
      901 tatccgctcc gcggcctagg ctagagtccg gaggctggat cggtcccggt gtcttctatg
      961 gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct
     1021 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     1081 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     1141 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     1201 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     1261 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     1321 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     1381 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     1441 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     1501 atttaccgta agttatgtaa cgacctgcac gatgctgttt cctgtgtgaa attgttatcc
     1561 gctcacaatt ccacacatta tacgagccgg aagctataaa gtgtaaagcc tggggtgcct
     1621 aatgagtgaa agggcctcgt atacgcctat ttttataggt taatgtcatg ataataatgg
     1681 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1741 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1801 aataatattg aaaaacgcgc gaattgcaag ctctgcatta atgaatcggc caacgcgcgg
     1861 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1921 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1981 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2041 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2101 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2161 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2221 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     2281 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2341 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2401 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2461 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     2521 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2581 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2641 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2701 acgaaaactc acgttaaggg attttggtca tgccataact tcgtatagca tacattatac
     2761 gaagttatgg catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3781 tgccaggggt gggcacacat atttgatacc agcgatccct acacagcaca taattcaatg
     3841 cgacttccct ctatcgcaca tcttagacct ttattctccc tccagcacac atcgaagctg
     3901 ccgagcaagc cgttctcacc agtccaagac ctggcatgag cggatacata tttgaatgta
     3961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
     4021 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4081 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     4141 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     4201 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4261 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     4321 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     4381 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     4441 ccgccgcgct taatgcgccg ctacagggcg cgtc
//
