LOCUS V040438 6538 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040438 VERSION V040438 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS complement(180..248) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(249..353) /gene="bla" /label="AmpR promoter" rep_origin 380..835 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" primer_bind 976..992 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 1153..1532 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 1533..1736 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 1772..1798 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1802..1828 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1832..1858 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1880..3691 /label="BIRC3(NM_182962)" /note="BIRC3(NM_182962)" /gene="BIRC3" polyA_signal 3714..4336 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" promoter 4365..4694 /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(4733..4751) /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" primer_bind complement(4765..4781) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4789..4805 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4813..4843) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4858..4879 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5167..5755) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 61 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 361 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 421 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 481 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 541 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 601 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 661 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 721 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 781 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt 841 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 901 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt 961 cccagtcacg acgttgtaaa acgacggcca gtgccaagct gatctataca ttgaatcaat 1021 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc 1081 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat 1141 gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat 1201 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg 1261 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa 1321 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact 1381 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta 1441 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt 1501 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg 1561 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg 1621 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc 1681 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt 1741 tagtgaaccg tcagaattaa ccgccaccat gtacccatac gacgtacctg attatgcaac 1801 ttatccttat gatgtacctg attatgctag ttatccttat gatgtcccag actacgcaaa 1861 gcttgcggcc gcgaattcaa tgaacatagt agaaaacagc atattcttat caaatttgat 1921 gaaaagcgcc aacacgtttg aactgaaata cgacttgtca tgtgaactgt accgaatgtc 1981 tacgtattcc acttttcctg ctggggttcc tgtctcagaa aggagtcttg ctcgtgctgg 2041 tttctattac actggtgtga atgacaaggt caaatgcttc tgttgtggcc tgatgctgga 2101 taactggaaa agaggagaca gtcctactga aaagcataaa aagttgtatc ctagctgcag 2161 attcgttcag agtctaaatt ccgttaacaa cttggaagct acctctcagc ctacttttcc 2221 ttcttcagta acaaattcca cacactcatt acttccgggt acagaaaaca gtggatattt 2281 ccgtggctct tattcaaact ctccatcaaa tcctgtaaac tccagagcaa atcaagattt 2341 ttctgccttg atgagaagtt cctaccactg tgcaatgaat aacgaaaatg ccagattact 2401 tacttttcag acatggccat tgacttttct gtcgccaaca gatctggcaa aagcaggctt 2461 ttactacata ggacctggag acagagtggc ttgctttgcc tgtggtggaa aattgagcaa 2521 ttgggaaccg aaggataatg ctatgtcaga acacctgaga cattttccca aatgcccatt 2581 tatagaaaat cagcttcaag acacttcaag atacacagtt tctaatctga gcatgcagac 2641 acatgcagcc cgctttaaaa cattctttaa ctggccctct agtgttctag ttaatcctga 2701 gcagcttgca agtgcgggtt tttattatgt gggtaacagt gatgatgtca aatgcttttg 2761 ctgtgatggt ggactcaggt gttgggaatc tggagatgat ccatgggttc aacatgccaa 2821 gtggtttcca aggtgtgagt acttgataag aattaaagga caggagttca tccgtcaagt 2881 tcaagccagt taccctcatc tacttgaaca gctgctatcc acatcagaca gcccaggaga 2941 tgaaaatgca gagtcatcaa ttatccattt tgaacctgga gaagaccatt cagaagatgc 3001 aatcatgatg aatactcctg tgattaatgc tgccgtggaa atgggcttta gtagaagcct 3061 ggtaaaacag acagttcaga gaaaaatcct agcaactgga gagaattata gactagtcaa 3121 tgatcttgtg ttagacttac tcaatgcaga agatgaaata agggaagagg agagagaaag 3181 agcaactgag gaaaaagaat caaatgattt attattaatc cggaagaata gaatggcact 3241 ttttcaacat ttgacttgtg taattccaat cctggatagt ctactaactg ccggaattat 3301 taatgaacaa gaacatgatg ttattaaaca gaagacacag acgtctttac aagcaagaga 3361 actgattgat acgattttag taaaaggaaa tattgcagcc actgtattca gaaactctct 3421 gcaagaagct gaagctgtgt tatatgagca tttatttgtg caacaggaca taaaatatat 3481 tcccacagaa gatgtttcag atctaccagt ggaagaacaa ttgcggagac tacaagaaga 3541 aagaacatgt aaagtgtgta tggacaaaga agtgtccata gtgtttattc cttgtggtca 3601 tctagtagta tgcaaagatt gtgctccttc tttaagaaag tgtcctattt gtaggagtac 3661 aatcaagggt acagttcgta catttctttc atgagtcgac tctagaggat cccgggtggc 3721 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca 3781 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata 3841 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta 3901 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg 3961 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat 4021 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca 4081 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc 4141 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa 4201 aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc catgcccaac 4261 cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg tccactcagt 4321 agatgcctgt tgaattgggt acgcggccag cttggctgtg gaatgtgtgt cagttagggt 4381 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 4441 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 4501 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 4561 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 4621 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 4681 taggcttttg caaaaagctc ctcgaggaac tgaaaaacca gaaagttaat tccctatagt 4741 gagtcgtatt aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct 4801 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 4861 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 4921 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 4981 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 5041 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 5101 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 5161 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 5221 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 5281 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 5341 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 5401 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 5461 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 5521 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 5581 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 5641 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 5701 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 5761 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 5821 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 5881 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 5941 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 6001 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 6061 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 6121 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 6181 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 6241 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 6301 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 6361 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 6421 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 6481 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcg //