LOCUS       V040438                 6538 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040438
VERSION     V040438
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(180..248)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(249..353)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      380..835
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     976..992
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        1153..1532
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        1533..1736
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             1772..1798
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1802..1828
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1832..1858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1880..3691
                     /label="BIRC3(NM_182962)"
                     /note="BIRC3(NM_182962)"
                     /gene="BIRC3"
     polyA_signal    3714..4336
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     promoter        4365..4694
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4733..4751)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(4765..4781)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4789..4805
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4813..4843)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4858..4879
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5167..5755)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
       61 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      361 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      421 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      481 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      541 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      601 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      661 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      721 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
      781 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt
      841 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      901 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
      961 cccagtcacg acgttgtaaa acgacggcca gtgccaagct gatctataca ttgaatcaat
     1021 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     1081 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     1141 gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     1201 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     1261 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     1321 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     1381 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta
     1441 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt
     1501 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg
     1561 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1621 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc
     1681 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     1741 tagtgaaccg tcagaattaa ccgccaccat gtacccatac gacgtacctg attatgcaac
     1801 ttatccttat gatgtacctg attatgctag ttatccttat gatgtcccag actacgcaaa
     1861 gcttgcggcc gcgaattcaa tgaacatagt agaaaacagc atattcttat caaatttgat
     1921 gaaaagcgcc aacacgtttg aactgaaata cgacttgtca tgtgaactgt accgaatgtc
     1981 tacgtattcc acttttcctg ctggggttcc tgtctcagaa aggagtcttg ctcgtgctgg
     2041 tttctattac actggtgtga atgacaaggt caaatgcttc tgttgtggcc tgatgctgga
     2101 taactggaaa agaggagaca gtcctactga aaagcataaa aagttgtatc ctagctgcag
     2161 attcgttcag agtctaaatt ccgttaacaa cttggaagct acctctcagc ctacttttcc
     2221 ttcttcagta acaaattcca cacactcatt acttccgggt acagaaaaca gtggatattt
     2281 ccgtggctct tattcaaact ctccatcaaa tcctgtaaac tccagagcaa atcaagattt
     2341 ttctgccttg atgagaagtt cctaccactg tgcaatgaat aacgaaaatg ccagattact
     2401 tacttttcag acatggccat tgacttttct gtcgccaaca gatctggcaa aagcaggctt
     2461 ttactacata ggacctggag acagagtggc ttgctttgcc tgtggtggaa aattgagcaa
     2521 ttgggaaccg aaggataatg ctatgtcaga acacctgaga cattttccca aatgcccatt
     2581 tatagaaaat cagcttcaag acacttcaag atacacagtt tctaatctga gcatgcagac
     2641 acatgcagcc cgctttaaaa cattctttaa ctggccctct agtgttctag ttaatcctga
     2701 gcagcttgca agtgcgggtt tttattatgt gggtaacagt gatgatgtca aatgcttttg
     2761 ctgtgatggt ggactcaggt gttgggaatc tggagatgat ccatgggttc aacatgccaa
     2821 gtggtttcca aggtgtgagt acttgataag aattaaagga caggagttca tccgtcaagt
     2881 tcaagccagt taccctcatc tacttgaaca gctgctatcc acatcagaca gcccaggaga
     2941 tgaaaatgca gagtcatcaa ttatccattt tgaacctgga gaagaccatt cagaagatgc
     3001 aatcatgatg aatactcctg tgattaatgc tgccgtggaa atgggcttta gtagaagcct
     3061 ggtaaaacag acagttcaga gaaaaatcct agcaactgga gagaattata gactagtcaa
     3121 tgatcttgtg ttagacttac tcaatgcaga agatgaaata agggaagagg agagagaaag
     3181 agcaactgag gaaaaagaat caaatgattt attattaatc cggaagaata gaatggcact
     3241 ttttcaacat ttgacttgtg taattccaat cctggatagt ctactaactg ccggaattat
     3301 taatgaacaa gaacatgatg ttattaaaca gaagacacag acgtctttac aagcaagaga
     3361 actgattgat acgattttag taaaaggaaa tattgcagcc actgtattca gaaactctct
     3421 gcaagaagct gaagctgtgt tatatgagca tttatttgtg caacaggaca taaaatatat
     3481 tcccacagaa gatgtttcag atctaccagt ggaagaacaa ttgcggagac tacaagaaga
     3541 aagaacatgt aaagtgtgta tggacaaaga agtgtccata gtgtttattc cttgtggtca
     3601 tctagtagta tgcaaagatt gtgctccttc tttaagaaag tgtcctattt gtaggagtac
     3661 aatcaagggt acagttcgta catttctttc atgagtcgac tctagaggat cccgggtggc
     3721 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     3781 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     3841 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     3901 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     3961 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     4021 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     4081 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     4141 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa
     4201 aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc catgcccaac
     4261 cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg tccactcagt
     4321 agatgcctgt tgaattgggt acgcggccag cttggctgtg gaatgtgtgt cagttagggt
     4381 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4441 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4501 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4561 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4621 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4681 taggcttttg caaaaagctc ctcgaggaac tgaaaaacca gaaagttaat tccctatagt
     4741 gagtcgtatt aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     4801 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4861 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4921 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4981 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5041 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5101 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5161 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5221 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5281 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5341 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5401 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5461 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5521 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5581 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5641 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5701 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5761 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5821 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5881 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5941 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6001 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6061 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6121 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6181 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6241 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6301 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6361 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6421 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6481 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcg
//
