LOCUS       V036138                 7157 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036138
VERSION     V036138
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     141..157
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        318..697
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        698..901
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             935..958
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             962..2947
                     /label="DDX3X(NM_001356)"
                     /note="DDX3X(NM_001356)"
                     /gene="DDX3X"
     CDS             2969..3487
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNG
                     IKANFKIRHNIE"
                     /label="VN173"
     polyA_signal    3498..4120
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     promoter        4149..4478
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4517..4535)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(4549..4565)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4573..4589
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4597..4627)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4642..4663
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(4951..5539)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5710..6570)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6571..6675)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6702..7130
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
      961 tatgagtcat gtggcagtgg aaaatgcgct cgggctggac cagcagtttg ctggcctaga
     1021 cctgaactct tcagataatc agagtggagg aagtacagcc agcaaagggc gctatattcc
     1081 tcctcattta aggaaccgag aagctactaa aggtttctac gataaagaca gttcagggtg
     1141 gagttctagc aaagataagg atgcgtatag cagttttgga tctcgtagtg attcaagagg
     1201 gaagtctagc ttcttcagtg atcgtggaag tggatcaagg ggaaggtttg atgatcgtgg
     1261 acggagtgat tacgatggca ttggcagccg tggtgacaga agtggctttg gcaaatttga
     1321 acgtggtgga aacagtcgct ggtgtgacaa atcagatgaa gatgattggt caaaaccact
     1381 cccaccaagt gaacgcttgg aacaggaact cttttctgga ggcaacactg ggattaattt
     1441 tgagaaatac gatgacattc cagttgaggc aacaggcaac aactgtcctc cacatattga
     1501 aagtttcagt gatgttgaga tgggagaaat tatcatggga aacattgagc ttactcgtta
     1561 tactcgccca actccagtgc aaaagcatgc tattcctatt atcaaagaga aaagagactt
     1621 gatggcttgt gcccaaacag ggtctggaaa aactgcagca tttctgttgc ccatcttgag
     1681 tcagatttat tcagatggtc caggcgaggc tttgagggcc atgaaggaaa atggaaggta
     1741 tgggcgccgc aaacaatacc caatctcctt ggtattagca ccaacgagag agttggcagt
     1801 acagatctac gaggaagcca gaaaattttc ataccgatct agagttcgtc cttgcgtggt
     1861 ttatggtggt gccgatattg gtcagcagat tcgagacttg gaacgtggat gccatttgtt
     1921 agtagccact ccaggacgtc tagtggatat gatggaaaga ggaaagattg gattagactt
     1981 ttgcaaatac ttggtgttag atgaagctga tcggatgttg gatatggggt ttgagcctca
     2041 gattcgtaga atagtcgaac aagatactat gcctccaaag ggtgtccgcc acactatgat
     2101 gtttagtgct acttttccta aggaaataca gatgctggct cgtgatttct tagatgaata
     2161 tatcttcttg gctgtaggaa gagttggctc tacctctgaa aacatcacac agaaagtagt
     2221 ttgggtggaa gaatcagaca aacggtcatt tctgcttgac ctcctaaatg caacaggcaa
     2281 ggattcactg accttagtgt ttgtggagac caaaaagggt gcagattctc tggaggattt
     2341 cttataccat gaaggatacg catgtaccag catccatgga gaccgttctc agagggatag
     2401 agaagaggcc cttcaccagt tccgctcagg aaaaagccca attttagtgg ctacagcagt
     2461 agcagcaaga ggactggaca tttcaaatgt gaaacatgtt atcaattttg acttgccaag
     2521 tgatattgaa gaatatgtac atcgtattgg tcgtacggga cgtgtaggaa accttggcct
     2581 ggcaacctca ttctttaacg agaggaacat aaatattact aaggatttgt tggatcttct
     2641 tgttgaagct aaacaagaag tgccgtcttg gttagaaaac atggcttatg aacaccacta
     2701 caagggtagc agtcgtggac gttctaagag tagcagattt agtggagggt ttggtgccag
     2761 agactaccga caaagtagcg gtgccagcag ttccagcttc agcagcagcc gcgcaagcag
     2821 cagccgcagt ggcggaggtg gccacggtag cagcagagga tttggtggag gtggctatgg
     2881 aggcttttac aacagtgatg gatatggagg aaattataac tcccaggggg ttgactggtg
     2941 gggtaactct agaagatcca tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     3001 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3061 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac
     3121 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg
     3181 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3241 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3301 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3361 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3421 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     3481 catcgagtag ggatcccggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     3541 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     3601 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     3661 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     3721 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     3781 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     3841 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     3901 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     3961 ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc cagacacagc
     4021 ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt ggcactgtcc
     4081 tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg ccagcttggc
     4141 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     4201 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     4261 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     4321 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4381 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4441 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcctcgag gaactgaaaa
     4501 accagaaagt taattcccta tagtgagtcg tattaaattc gtaatcatgt catagctgtt
     4561 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4621 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4681 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4741 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4801 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4861 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4921 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4981 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5041 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5101 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5161 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5221 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5281 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5341 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5401 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5461 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5521 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5581 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5701 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     5761 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     5821 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     5881 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     5941 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6001 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6061 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6121 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6181 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6241 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6301 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6361 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6421 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6481 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6541 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6601 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6661 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
     6721 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6781 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6841 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6901 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6961 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7021 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     7081 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     7141 attaacgctt acaattt
//
