LOCUS V033069 7742 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V033069 VERSION V033069 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS 8..31 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" CDS 47..2575 /label="FBXO11(NM_025133)" /note="FBXO11(NM_025133)" /gene="FBXO11" CDS 2627..3145 /codon_start=1 /product="N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG IKANFKIRHNIE" /label="VN173" polyA_signal 3156..3778 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" promoter 3807..4136 /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(4175..4193) /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" primer_bind complement(4207..4223) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4231..4247 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4255..4285) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4300..4321 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4609..5197) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5368..6228) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6229..6333) /gene="bla" /label="AmpR promoter" rep_origin 6360..6815 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" primer_bind 6956..6972 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 7133..7512 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 7513..7716 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" ORIGIN 1 taccatggac tacaaagacg atgacgacaa gcttgcggcc gcgaatatgg ttgcagaaga 61 atcaggtcct ggtgcacaaa atagtccata ccaacttcgt agaaaaactc ttttgccgaa 121 aagaacagcg tgtcccacaa agaacagtat ggagggcgcc tcaacttcaa ctacagaaaa 181 ctttggtcat cgtgcaaaac gtgcaagagt gtctggaaaa tcacaagatc tatcagcagc 241 acctgctgaa cagtatcttc aggagaaact gccagatgaa gtggttctaa aaatcttctc 301 ttacttgctg gaacaggatc tttgtagagc agcttgtgta tgtaaacgct tcagtgaact 361 tgctaatgat ccaattttgt ggaaacgatt atatatggaa gtatttgaat atactcgccc 421 tatgatgcat cctgaacctg gaaaattcta ccagattaat ccagaagagt atgaacatcc 481 aaatccctgg aaagagagtt tccagcagtt gtataaaggt gcacatgtaa agccaggatt 541 tgctgaacat ttctacagta accctgcaag atataaagga agagaaaata tgttgtatta 601 tgatactatt gaagatgccc ttggtggggt acaagaggct cattttgatg gacttatctt 661 tgttcattct ggaatatata ctgatgaatg gatatatatt gaatctccaa tcaccatgat 721 tggtgcagca cctgggaaag tggcagacaa agttataatt gaaaacacta gagattcaac 781 cttcgttttt atggaaggct ctgaagatgc ttatgttgga tatatgacaa taaggtttaa 841 ccctgatgac aaatctgcac aacaccacaa tgcacaccac tgcttagaga ttacagtaaa 901 ttgtagccct attattgatc actgtatcat ccgaagtaca tgtacagttg gttctgcagt 961 atgtgttagt ggtcaaggag catgtcccac catcaagcac tgtaacatca gtgactgtga 1021 aaatgttgga ctatatataa cagatcatgc acagggaata tatgaggata atgaaatttc 1081 caataatgcg ttagctggga tttgggttaa aaatcatgga aacccaatta ttagacggaa 1141 tcatattcat catggacgtg atgttggtgt gttcacattt gatcatggca tgggttactt 1201 tgaaagttgc aatatacaca gaaataggat agcaggcttt gaagtaaaag cctatgctaa 1261 ccctacagtg gttcgatgtg aaattcacca tgggcagact ggaggaatat atgtccatga 1321 aaaaggaaga ggacaattca tagagaataa aatctatgca aacaactttg caggtgtatg 1381 gattacctca aatagtgacc caacaataag gggaaattct atatttaatg gaaatcaagg 1441 aggagtttac atctttggtg atggacgagg ccttattgaa ggaaatgaca tttatggcaa 1501 tgcattagca ggaattcaaa ttaggacaaa cagttgtcca attgttcggc ataacaaaat 1561 tcatgatggc cagcatggtg ggatttatgt gcatgaaaag ggacaaggag taatagaaga 1621 gaatgaagtt tatagtaaca ctctagctgg agtctgggtg acaactggca gcactccagt 1681 actgagaaga aaccggatac acagtggcaa gcaggttggt gtttattttt atgacaatgg 1741 acatggagtg ctagaagaca atgatatcta taatcatatg tattcagggg ttcagataag 1801 gactggaagc aaccccaaaa ttagacgcaa caaaatctgg ggaggacaga atggtggaat 1861 tctagtttat aattctggtc taggctgtat agaagacaat gaaatatttg acaatgcaat 1921 ggctggagtc tggattaaga cagatagtaa tcctacacta agaagaaata aaatccatga 1981 tggaagagat ggtggcatct gtatatttaa tgggggtcga ggtctccttg aagaaaatga 2041 tattttcagg aatgctcaag caggtgttct catcagcact aatagtcatc caatcttaag 2101 gaaaaacaga atatttgatg gatttgccgc aggtattgaa attacaaatc acgcaactgc 2161 aacactagaa ggcaatcaga tttttaacaa ccggtttgga ggcttatttt tagcatctgg 2221 tgttaatgtg acaatgaaag ataacaaaat aatgaacaat caagatgcca tagaaaaggc 2281 tgttagtaga ggccaatgtt tatataaaat atcaagttat accagctatc ccatgcatga 2341 tttctacaga tgtcatactt gtaacaccac agatcgaaat gccatatgtg tgaactgcat 2401 taagaagtgc catcagggac atgatgtaga gtttattaga catgataggt ttttctgtga 2461 ctgtggtgct ggaacactgt ctaatccttg tacattagct ggtgagccta cacatgatac 2521 agatacacta tatgactctg ctccacctat agaatctaat acattgcagc acaacggagg 2581 tggaggttca ggtggtggag gatcttctag aagatccagc gccaccatgg tgagcaaggg 2641 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 2701 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 2761 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 2821 gacctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt 2881 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 2941 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 3001 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 3061 cgccatcagc gacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa 3121 cttcaagatc cgccacaaca tcgagtaggg atcccgggtg gcatccctgt gacccctccc 3181 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa 3241 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg 3301 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt 3361 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg 3421 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca 3481 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg 3541 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac 3601 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta taccagcagg 3661 aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga catttgagtt 3721 gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct gttgaattgg 3781 gtacgcggcc agcttggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 3841 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 3901 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 3961 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 4021 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 4081 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 4141 tcctcgagga actgaaaaac cagaaagtta attccctata gtgagtcgta ttaaattcgt 4201 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 4261 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 4321 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 4381 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 4441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 4501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 4561 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 4621 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 4681 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 4741 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 4801 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 4861 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 4921 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 4981 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 5041 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 5101 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 5161 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 5221 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 5281 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 5341 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 5401 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 5461 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 5521 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 5581 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 5641 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 5701 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 5761 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 5821 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 5881 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 5941 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 6001 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 6061 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 6121 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 6181 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 6241 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 6301 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 6361 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 6421 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 6481 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 6541 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 6601 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 6661 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 6721 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 6781 cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac 6841 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga 6901 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa 6961 acgacggcca gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta 7021 gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta 7081 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga 7141 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 7201 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 7261 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 7321 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 7381 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 7441 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc 7501 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac 7561 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 7621 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt 7681 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaattga 7741 tc //