LOCUS       V038039                 7093 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038039
VERSION     V038039
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        join(6860..7093,1..96)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(135..153)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(167..183)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    191..207
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(215..245)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    260..281
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(569..1157)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(1328..2188)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(2189..2293)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      2320..2775
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     2916..2932
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        3093..3472
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        3473..3676
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             3721..5658
                     /label="HSPA8(NM_006597)"
                     /note="HSPA8(NM_006597)"
                     /gene="HSPA8"
     CDS             5680..6198
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNG
                     IKANFKIRHNIE"
                     /label="VN173"
     polyA_signal    6209..6831
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
ORIGIN
        1 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
       61 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcctcgagga actgaaaaac
      121 cagaaagtta attccctata gtgagtcgta ttaaattcgt aatcatgtca tagctgtttc
      181 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      241 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      301 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      361 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      421 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2221 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2281 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     2341 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2401 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2461 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2521 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2581 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2641 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2701 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2761 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     2821 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     2881 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
     2941 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
     3001 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
     3061 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
     3121 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     3181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     3241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     3301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
     3361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
     3421 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     3481 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     3541 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     3601 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
     3661 ctatataagc agagctcgtt tagtgaaccg tcagaattga tctaccatgc ttgcggccgc
     3721 atgtccaagg gacctgcagt tggtattgat cttggcacca cctactcttg tgtgggtgtt
     3781 ttccagcacg gaaaagtcga gataattgcc aatgatcagg gaaaccgaac cactccaagc
     3841 tatgtcgcct ttacggacac tgaacggttg atcggtgatg ccgcaaagaa tcaagttgca
     3901 atgaacccca ccaacacagt ttttgatgcc aaacgtctga ttggacgcag atttgatgat
     3961 gctgttgtcc agtctgatat gaaacattgg ccctttatgg tggtgaatga tgctggcagg
     4021 cccaaggtcc aagtagaata caagggagag accaaaagct tctatccaga ggaggtgtct
     4081 tctatggttc tgacaaagat gaaggaaatt gcagaagcct accttgggaa gactgttacc
     4141 aatgctgtgg tcacagtgcc agcttacttt aatgactctc agcgtcaggc taccaaagat
     4201 gctggaacta ttgctggtct caatgtactt agaattatta atgagccaac tgctgctgct
     4261 attgcttacg gcttagacaa aaaggttgga gcagaaagaa acgtgctcat ctttgacctg
     4321 ggaggtggca cttttgatgt gtcaatcctc actattgagg atggaatctt tgaggtcaag
     4381 tctacagctg gagacaccca cttgggtgga gaagattttg acaaccgaat ggtcaaccat
     4441 tttattgctg agtttaagcg caagcataag aaggacatca gtgagaacaa gagagctgta
     4501 agacgcctcc gtactgcttg tgaacgtgct aagcgtaccc tctcttccag cacccaggcc
     4561 agtattgaga tcgattctct ctatgaagga atcgacttct atacctccat tacccgtgcc
     4621 cgatttgaag aactgaatgc tgacctgttc cgtggcaccc tggacccagt agagaaagcc
     4681 cttcgagatg ccaaactaga caagtcacag attcatgata ttgtcctggt tggtggttct
     4741 actcgtatcc ccaagattca gaagcttctc caagacttct tcaatggaaa agaactgaat
     4801 aagagcatca accctgatga agctgttgct tatggtgcag ctgtccaggc agccatcttg
     4861 tctggagaca agtctgagaa tgttcaagat ttgctgctct tggatgtcac tcctctttcc
     4921 cttggtattg aaactgctgg tggagtcatg actgtcctca tcaagcgtaa taccaccatt
     4981 cctaccaagc agacacagac cttcactacc tattctgaca accagcctgg tgtgcttatt
     5041 caggtttatg aaggcgagcg tgccatgaca aaggataaca acctgcttgg caagtttgaa
     5101 ctcacaggca tacctcctgc accccgaggt gttcctcaga ttgaagtcac ttttgacatt
     5161 gatgccaatg gtatactcaa tgtctctgct gtggacaaga gtacgggaaa agagaacaag
     5221 attactatca ctaatgacaa gggccgtttg agcaaggaag acattgaacg tatggtccag
     5281 gaagctgaga agtacaaagc tgaagatgag aagcagaggg acaaggtgtc atccaagaat
     5341 tcacttgagt cctatgcctt caacatgaaa gcaactgttg aagatgagaa acttcaaggc
     5401 aagattaacg atgaggacaa acagaagatt ctggacaagt gtaatgaaat tatcaactgg
     5461 cttgataaga atcagactgc tgagaaggaa gaatttgaac atcaacagaa agagctggag
     5521 aaagtttgca accccatcat caccaagctg taccagagtg caggaggcat gccaggagga
     5581 atgcctgggg gatttcctgg tggtggagct cctccctctg gtggtgcttc ctcagggccc
     5641 accattgaag aggttgattc tagaagatcc atcgccacca tggtgagcaa gggcgaggag
     5701 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     5761 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
     5821 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac
     5881 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     5941 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     6001 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     6061 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     6121 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     6181 atccgccaca acatcgagta gggatcccgg gtggcatccc tgtgacccct ccccagtgcc
     6241 tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag
     6301 ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt
     6361 atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc
     6421 aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc
     6481 gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag
     6541 ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa
     6601 ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg
     6661 aaccactgct cccttccctg tccttctgat tttaaaataa ctataccagc aggaggacgt
     6721 ccagacacag cataggctac ctggccatgc ccaaccggtg ggacatttga gttgcttgct
     6781 tggcactgtc ctctcatgcg ttgggtccac tcagtagatg cctgttgaat tgggtacgcg
     6841 gccagcttgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6901 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6961 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7021 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7081 ccatggctga cta
//
