LOCUS       V047494                 8638 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047494
VERSION     V047494
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..4558
                     /label="ACE(NM_000789)"
                     /note="ACE(NM_000789)"
                     /gene="ACE"
     CDS             4595..5305
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    5426..5547
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5554..6009)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6036..6140
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        6142..6499
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6534..7328
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7560..7607
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7936..8524
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgggggccg cctcgggccg
      661 ccgggggccg gggctgctgc tgccgctgcc gctgctgttg ctgctgccgc cgcagcccgc
      721 cctggcgttg gaccccgggc tgcagcccgg caacttttct gctgacgagg ccggggcgca
      781 gctcttcgcg cagagctaca actccagcgc cgaacaggtg ctgttccaga gcgtggccgc
      841 cagctgggcg cacgacacca acatcaccgc ggagaatgca aggcgccagg aggaagcagc
      901 cctgctcagc caggagtttg cggaggcctg gggccagaag gccaaggagc tgtatgaacc
      961 gatctggcag aacttcacgg acccgcagct gcgcaggatc atcggagctg tgcgcaccct
     1021 gggctctgcc aacctgcccc tggctaagcg gcagcagtac aacgccctgc taagcaacat
     1081 gagcaggatc tactccaccg ccaaggtctg cctccccaac aagactgcca cctgctggtc
     1141 cctggaccca gatctcacca acatcctggc ttcctcgcga agctacgcca tgctcctgtt
     1201 tgcctgggag ggctggcaca acgctgcggg catcccgctg aaaccgctgt acgaggattt
     1261 cactgccctc agcaatgaag cctacaagca ggacggcttc acagacacgg gggcctactg
     1321 gcgctcctgg tacaactccc ccaccttcga ggacgatctg gaacacctct accaacagct
     1381 agagcccctc tacctgaacc tccatgcctt cgtccgccgc gcactgcatc gccgatacgg
     1441 agacagatac atcaacctca ggggacccat ccctgctcat ctgctgggag acatgtgggc
     1501 ccagagctgg gaaaacatct acgacatggt ggtgcctttc ccagacaagc ccaacctcga
     1561 tgtcaccagt actatgctgc agcagggctg gaacgccacg cacatgttcc gggtggcaga
     1621 ggagttcttc acctccctgg agctctcccc catgcctccc gagttctggg aagggtcgat
     1681 gctggagaag ccggccgacg ggcgggaagt ggtgtgccac gcctcggctt gggacttcta
     1741 caacaggaaa gacttcagga tcaagcagtg cacacgggtc acgatggacc agctctccac
     1801 agtgcaccat gagatgggcc atatacagta ctacctgcag tacaaggatc tgcccgtctc
     1861 cctgcgtcgg ggggccaacc ccggcttcca tgaggccatt ggggacgtgc tggcgctctc
     1921 ggtctccact cctgaacatc tgcacaaaat cggcctgctg gaccgtgtca ccaatgacac
     1981 ggaaagtgac atcaattact tgctaaaaat ggcactggaa aaaattgcct tcctgccctt
     2041 tggctacttg gtggaccagt ggcgctgggg ggtctttagt gggcgtaccc ccccttcccg
     2101 ctacaacttc gactggtggt atcttcgaac caagtatcag gggatctgtc ctcctgttac
     2161 ccgaaacgaa acccactttg atgctggagc taagtttcat gttccaaatg tgacaccata
     2221 catcaggtac tttgtgagtt ttgtcctgca gttccagttc catgaagccc tgtgcaagga
     2281 ggcaggctat gagggcccac tgcaccagtg tgacatctac cggtccacca aggcaggggc
     2341 caagctccgg aaggtgctgc aggctggctc ctccaggccc tggcaggagg tgctgaagga
     2401 catggtcggc ttagatgccc tggatgccca gccgctgctc aagtacttcc agccagtcac
     2461 ccagtggctg caggagcaga accagcagaa cggcgaggtc ctgggctggc ccgagtacca
     2521 gtggcacccg ccgttgcctg acaactaccc ggagggcata gacctggtga ctgatgaggc
     2581 tgaggccagc aagtttgtgg aggaatatga ccggacatcc caggtggtgt ggaacgagta
     2641 tgccgaggcc aactggaact acaacaccaa catcaccaca gagaccagca agattctgct
     2701 gcagaagaac atgcaaatag ccaaccacac cctgaagtac ggcacccagg ccaggaagtt
     2761 tgatgtgaac cagttgcaga acaccactat caagcggatc ataaagaagg ttcaggacct
     2821 agaacgggca gcactgcctg cccaggagct ggaggagtac aacaagatcc tgttggatat
     2881 ggaaaccacc tacagcgtgg ccactgtgtg ccacccgaat ggcagctgcc tgcagctcga
     2941 gccagatctg acgaatgtga tggccacgtc ccggaaatat gaagacctgt tatgggcatg
     3001 ggagggctgg cgagacaagg cggggagagc catcctccag ttttacccga aatacgtgga
     3061 actcatcaac caggctgccc ggctcaatgg ctatgtagat gcaggggact cgtggaggtc
     3121 tatgtacgag acaccatccc tggagcaaga cctggagcgg ctcttccagg agctgcagcc
     3181 actctacctc aacctgcatg cctacgtgcg ccgggccctg caccgtcact acggggccca
     3241 gcacatcaac ctggaggggc ccattcctgc tcacctgctg gggaacatgt gggcgcagac
     3301 ctggtccaac atctatgact tggtggtgcc cttcccttca gccccctcga tggacaccac
     3361 agaggctatg ctaaagcagg gctggacgcc caggaggatg tttaaggagg ctgatgattt
     3421 cttcacctcc ctggggctgc tgcccgtgcc tcctgagttc tggaacaagt cgatgctgga
     3481 gaagccaacc gacgggcggg aggtggtctg ccacgcctcg gcctgggact tctacaacgg
     3541 caaggacttc cggatcaagc agtgcaccac cgtgaacttg gaggacctgg tggtggccca
     3601 ccacgaaatg ggccacatcc agtatttcat gcagtacaaa gacttacctg tggccttgag
     3661 ggagggtgcc aaccccggct tccatgaggc cattggggac gtgctagccc tctcagtgtc
     3721 tacgcccaag cacctgcaca gtctcaacct gctgagcagt gagggtggca gcgacgagca
     3781 tgacatcaac tttctgatga agatggccct tgacaagatc gcctttatcc ccttcagcta
     3841 cctcgtcgat cagtggcgct ggagggtatt tgatggaagc atcaccaagg agaactataa
     3901 ccaggagtgg tggagcctca ggctgaagta ccagggcctc tgccccccag tgcccaggac
     3961 tcaaggtgac tttgacccag gggccaagtt ccacattcct tctagcgtgc cttacatcag
     4021 gtactttgtc agcttcatca tccagttcca gttccacgag gcactgtgcc aggcagctgg
     4081 ccacacgggc cccctgcaca agtgtgacat ctaccagtcc aaggaggccg ggcagcgcct
     4141 ggcgaccgcc atgaagctgg gcttcagtag gccgtggccg gaagccatgc agctgatcac
     4201 gggccagccc aacatgagcg cctcggccat gttgagctac ttcaagccgc tgctggactg
     4261 gctccgcacg gagaacgagc tgcatgggga gaagctgggc tggccgcagt acaactggac
     4321 gccgaactcc gctcgctcag aagggcccct cccagacagc ggccgcgtca gcttcctggg
     4381 cctggacctg gatgcgcagc aggcccgcgt gggccagtgg ctgctgctct tcctgggcat
     4441 cgccctgctg gtagccaccc tgggcctcag ccagcggctc ttcagcatcc gccaccgcag
     4501 cctccaccgg cactcccacg ggccccagtt cggctccgag gtggagctga gacactccgg
     4561 tggaggcgga tcaggaggcg gtggatctgg atccatggtg agcaagggcg aggaggataa
     4621 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     4681 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     4741 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     4801 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     4861 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     4921 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     4981 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     5041 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     5101 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     5161 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     5221 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     5281 cggcatggac gagctgtaca agtagcggcc gcgactctag atcataatca gccataccac
     5341 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5401 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5521 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     5581 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     5641 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     5701 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     5761 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     5821 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     5881 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     5941 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6001 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6061 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6121 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     6181 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6241 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6301 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6361 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6421 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6481 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     6541 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     6601 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     6661 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     6721 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     6781 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     6841 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     6901 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     6961 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     7021 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     7081 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     7141 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     7201 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     7261 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     7321 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     7381 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     7441 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     7501 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     7561 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     7621 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     7681 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     7741 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     7801 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7861 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7921 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7981 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8041 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     8101 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8161 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8221 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8281 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8341 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8401 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8461 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8521 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8581 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
