LOCUS       V046597                 6585 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046597
VERSION     V046597
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..2473
                     /label="AHCYL2(NM_015328)"
                     /note="AHCYL2(NM_015328)"
                     /gene="AHCYL2"
     CDS             2531..3250
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EYFP"
     polyA_signal    3373..3494
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3501..3956)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3983..4087
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4089..4446
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4481..5275
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5507..5554
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5883..6471
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgtcggtgc aggttgtgtc
      661 agccgcggct gccgccaagg tgcctgaggt ggagctgaag gacctgagcc cctccgaggc
      721 ggagtcgcaa ctaggactga gcacggccgc cgtgggcgcc atggcccccc cggcgggcgg
      781 tggagaccct gaggctccag ctcccgccgc ggagcggccc ccggtccccg gcccgggctc
      841 ggggcccgcc gccgctctca gccccgccgc cgggaaggtg cctcaggcgt cggccatgaa
      901 gcggagcgac ccacatcacc agcaccagcg gcaccgcgac ggcggcgagg ccctggtcag
      961 ccccgacggc accgtcaccg aggcgccgcg cacagtcaag aagcagatcc agtttgctga
     1021 ccagaagcaa gaattcaaca aacgtcccac caaaattgga cgtcgctctt tgtctcgttc
     1081 catttctcag tcatctactg acagctacag ctcagcggct tcatatacag atagctctga
     1141 tgatgagaca tcgcccaggg acaagcagca aaagaactct aagggaagca gtgacttctg
     1201 tgttaagaac atcaaacagg cagagtttgg acgaagagaa attgaaattg ctgaacaaga
     1261 aatgcctgca ttgatggctt tgaggaagag agctcaagga gaaaagcctt tggctggagc
     1321 caaaatcgtg ggttgcacac acatcactgc tcagactgct gtgcttatgg aaactctggg
     1381 tgctctgggg gcccagtgcc gatgggctgc ctgcaacatc tattccactc tcaatgaagt
     1441 ggctgctgct ctagcagaaa gtggatttcc tgtttttgcc tggaagggag agtcagaaga
     1501 tgacttttgg tggtgtatcg atagatgtgt gaatgtggag ggctggcagc caaacatgat
     1561 cttggatgat ggaggggatc ttacccactg gatttataaa aagtatccca acatgtttaa
     1621 gaaaatcaag ggcatagtag aggagagtgt tactggagtt cacaggctgt accaactgtc
     1681 caaagctggg aagctgtgtg ttccagccat gaatgtcaat gactcagtca ccaaacagaa
     1741 atttgacaac ctctactgtt gccgtgaatc aattcttgat ggacttaaaa ggacaacaga
     1801 catgatgttt ggtggaaagc aagtggtagt ctgtggctat ggagaggtgg ggaaagggtg
     1861 ctgtgctgcc ctgaaagcca tgggctccat tgtgtatgta actgaaattg accccatctg
     1921 tgccctgcaa gcctgtatgg atggatttcg actggtgaaa ttaaatgagg tcatccgaca
     1981 agtggacatt gttattacct gtacaggtaa caagaatgtg gtaaccagag agcacttgga
     2041 ccgtatgaag aatagctgca tcgtttgtaa catgggacat tccaacacag agattgacgt
     2101 ggcgagtctg cggacaccag aactgacctg ggagcgagtg agatctcaag ttgaccatgt
     2161 gatatggcct gatggcaaga ggatagtact gctggcagag ggccgcctgc tgaaccttag
     2221 ctgctccaca gtgcctacat ttgtgctctc aatcactgct actactcagg ctcttgcctt
     2281 gatagagctt tacaatgctc ctgagggtcg ctataagcag gatgtctacc tgttgcccaa
     2341 gaagatggat gagtatgtgg ccagcctaca cctgcctacc tttgatgccc acttgacaga
     2401 gctgacagat gaacaggcca agtatctggg actcaacaag aatgggccct tcaagcctaa
     2461 ttactacagg tatgaattcg gtggaggcgg atcaggaggc ggtggatctc cggatccacc
     2521 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2581 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2641 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2701 ctggcccacc ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga
     2761 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2821 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2881 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2941 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3001 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3061 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3121 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     3181 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     3241 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     3301 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     3361 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3421 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3481 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3541 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3601 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3661 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3721 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     3781 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3841 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     3901 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     3961 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4021 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4081 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     4141 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4201 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4261 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4321 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4381 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4441 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4501 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4561 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4621 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     4681 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4741 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4801 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4861 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4921 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4981 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     5041 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5101 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5161 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5221 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     5281 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     5341 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5401 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     5461 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     5521 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     5581 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     5641 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     5701 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     5761 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5821 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5881 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5941 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6001 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6061 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6121 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6181 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6241 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6301 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6361 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6421 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6481 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6541 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
