LOCUS V028055 5118 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028055 VERSION V028055 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..1015 /label="BATF(NM_006399)" /note="BATF(NM_006399)" /gene="BATF" CDS 1064..1783 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 1906..2027 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2034..2489) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2516..2620 /gene="bla" /label="AmpR promoter" promoter 2622..2979 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3014..3808 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4040..4087 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4416..5004 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atgcctcaca gctccgacag 661 cagtgactcc agcttcagcc gctctcctcc ccctggcaaa caggactcat ctgatgatgt 721 gagaagagtt cagaggaggg agaaaaatcg tattgccgcc cagaagagcc gacagaggca 781 gacacagaag gccgacaccc tgcacctgga gagcgaagac ctggagaaac agaacgcggc 841 tctacgcaag gagatcaagc agctcacaga ggaactgaag tacttcacgt cggtgctgaa 901 cagccacgag cccctgtgct cggtgctggc cgccagcacg ccctcgcccc ccgaggtggt 961 gtacagcgcc cacgcattcc accaacctca tgtcagctcc ccgcgcttcc agcccggtgg 1021 aggcggatca ggaggcggtg gatcggatcc accggtcgcc accatggtga gcaagggcga 1081 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 1141 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 1201 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 1261 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 1321 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 1381 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 1441 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 1501 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 1561 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 1621 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 1681 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 1741 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag 1801 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 1861 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 1921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 1981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt 2041 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 2101 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 2161 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 2221 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 2281 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 2341 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 2401 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 2461 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg 2521 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 2581 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag 2641 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 2701 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 2761 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 2821 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 2881 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 2941 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg 3001 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 3061 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 3121 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 3181 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 3241 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 3301 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 3361 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 3421 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 3481 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 3541 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 3601 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 3661 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 3721 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 3781 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 3841 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 3901 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 3961 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg 4021 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg 4081 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga 4141 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg 4201 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt 4261 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 4321 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 4381 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 4441 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 4501 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 4561 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 4621 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 4681 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 4741 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 4801 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 4861 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 4921 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 4981 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 5041 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 5101 cgtattaccg ccatgcat //