LOCUS       V026051                 6057 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026051
VERSION     V026051
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..1969
                     /label="FBXO7(NM_001033024)"
                     /note="FBXO7(NM_001033024)"
                     /gene="FBXO7"
     CDS             2003..2722
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2845..2966
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2973..3428)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3455..3559
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3561..3918
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3953..4747
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4979..5026
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5355..5943
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggcccggc ctcccggggg
      661 ctctggtccc ctcctcgatt cagagcattc ttcactccag aataatgagc aaccctcttt
      721 ggccaccagc tccaatcaga ctagcatgca ggatgaacaa ccaagtgatt cattccaagg
      781 acaggcagcc cagtctggtg tttggaatga cgacagtatg ttagggccta gtcaaaattt
      841 tgaagctgag tcaattcaag ataatgcgca tatggcagag ggcacaggtt tctatccctc
      901 agaacccatg ctctgtagtg aatcggtgga agggcaagtg ccacattcat tagagacctt
      961 gtatcaatca gctgactgtt ctgatgccaa tgatgccttg atagtgttga tacatcttct
     1021 catgttggag tcaggttaca tacctcaggg caccgaagcc aaagcactgt ccatgccgga
     1081 gaagtggaag ttgagcgggg tgtataagct gcagtacatg catcctctct gcgagggcag
     1141 ctccgctact ctcacctgtg tgcctttggg aaacctgatt gttgtaaatg ctacactaaa
     1201 aatcaacaat gagattagaa gtgtgaaaag attgcagctg ctaccagaat cttttatttg
     1261 caaagagaaa ctaggggaaa atgtagccaa catatacaaa gatcttcaga aactctctcg
     1321 cctctttaaa gaccagctgg tgtatcctct tctggctttt acccgacaag cactgaacct
     1381 accagatgta tttgggttgg tcgtcctccc attggaactg aaactacgga tcttccgact
     1441 tctggatgtt cgttccgtct tgtctttgtc tgcggtttgt cgtgacctct ttactgcttc
     1501 aaatgaccca ctcctgtgga ggtttttata tctgcgtgat tttcgagaca atactgtcag
     1561 agttcaagac acagattgga aagaactgta caggaagagg cacatacaaa gaaaagaatc
     1621 cccgaaaggg cggtttgtga tgctcctgcc atcgtcaact cacaccattc cattctatcc
     1681 caaccccttg caccctaggc catttcctag ctcccgcctt cctccaggaa ttatcggggg
     1741 tgaatatgac caaagaccaa cacttcccta tgttggagac ccaatcagtt cactcattcc
     1801 tggtcctggg gagacgccca gccagtttcc tccactgaga ccacgctttg atccagttgg
     1861 cccacttcca ggacctaacc ccatcttgcc agggcgaggc ggccccaatg acagatttcc
     1921 ctttagaccc agcaggggtc ggccaactga tggccggctg tcattcatgg gaggtggtgg
     1981 ctcggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2041 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2101 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2161 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     2221 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2281 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2341 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2401 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     2461 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     2521 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2581 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     2641 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2701 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     2761 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2821 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2881 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2941 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     3001 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3061 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3121 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3181 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3241 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3301 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3361 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3421 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3481 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3541 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     3601 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3661 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3721 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3781 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3841 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3901 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     3961 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4021 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4081 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4141 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4201 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4261 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4321 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4381 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4441 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     4501 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4561 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4621 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4681 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4741 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4801 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     4861 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     4921 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     4981 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     5041 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5101 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     5161 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5221 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5281 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5341 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5401 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5461 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5521 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5581 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5641 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5701 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5761 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5821 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5881 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5941 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6001 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
