LOCUS       V020719                 5988 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020719
VERSION     V020719
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..1897
                     /label="Irf3(NM_016849)"
                     /note="Irf3(NM_016849)"
                     /gene="Irf3"
     CDS             1934..2653
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2776..2897
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2904..3359)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3386..3490
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3492..3849
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3884..4678
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4910..4957
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5286..5874
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggaaaccc cgaaaccgcg
      661 gattttgccc tggctggtgt cacagctgga cctggggcag ctggaaggcg tggcctggct
      721 ggacgagagc cgaacgaggt tcaggatccc gtggaagcat ggcctacggc aggacgcaca
      781 gatggctgac tttggcatct tccaggcctg ggcagaagcc agtggtgcct acaccccggg
      841 gaaggataag ccggacgtgt caacctggaa gaggaatttc cggtcagccc tgaaccggaa
      901 agaagtgttg cggttagctg ctgacaatag caaggaccct tatgaccctc ataaagtgta
      961 tgagtttgtg actccagggg cgcgggactt cgtacatctg ggtgcctctc ctgacaccaa
     1021 tggcaaaagc agcctgcctc actcccagga aaacctaccg aagttatttg atggcctgat
     1081 cttggggccc ctcaaagatg aggggtcctc agatctggct attgtttctg atccttctca
     1141 acaactgcca agccccaatg tgaacaactt cctaaaccct gcaccccaag aaaatccact
     1201 gaagcagctg ctagctgagg aacaatggga gttcgaggtg accgccttct accgaggccg
     1261 ccaggtcttc cagcagacac tcttttgccc ggggggcctg cggctggtgg gcagcacagc
     1321 tgacatgaca ctgccctggc agccagtcac cctgcccgat cctgaggggt ttctgacgga
     1381 caagcttgtg aaggagtacg tggggcaggt gctcaaaggg ctgggcaatg ggctggcact
     1441 gtggcaggct gggcagtgcc tctgggccca gcgcctaggc cactcccacg ccttctgggc
     1501 tctgggggag gagctgcttc cagacagtgg gcgagggcct gatggagagg tccacaagga
     1561 caaggacgga gccgtgttcg acctcaggcc cttcgtggca gatctgattg ccttcatgga
     1621 aggaagtgga cactccccac gctacactct gtggttctgc atgggggaaa tgtggcccca
     1681 ggaccagcca tgggtcaaga ggcttgtgat ggtcaaggtt gttcctacat gtcttaagga
     1741 gctgttagag atggcccggg aagggggagc ctcttcactg aaaaccgtgg acttgcacat
     1801 ctccaacagc cagcctatct cccttacctc tgaccagtac aaggcctacc tccaggactt
     1861 ggtggaggac atggacttcc aggccactgg aaatatctcg gtaccgcggg cccgggatcc
     1921 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1981 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2041 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2101 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     2161 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2221 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2281 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2341 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     2401 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     2461 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2521 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     2581 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2641 gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag
     2701 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     2761 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     2821 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     2881 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     2941 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     3001 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     3061 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     3121 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     3181 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     3241 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     3301 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     3361 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3421 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3481 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     3541 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3601 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3661 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3721 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3781 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3841 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     3901 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3961 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     4021 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     4081 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     4141 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     4201 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     4261 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     4321 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     4381 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     4441 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     4501 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     4561 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     4621 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     4681 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     4741 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4801 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     4861 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     4921 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     4981 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     5041 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     5101 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     5161 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5221 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5281 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5341 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5401 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5461 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5521 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5581 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5641 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5701 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5761 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5821 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5881 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5941 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
