LOCUS V043305 7656 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043305 VERSION V043305 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..3550 /label="MYO19(NM_001163735)" /note="MYO19(NM_001163735)" /gene="MYO19" CDS 3602..4321 /codon_start=1 /note="mammalian codon-optimized" /product="enhanced YFP" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EYFP" polyA_signal 4444..4565 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4572..5027) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5054..5158 /gene="bla" /label="AmpR promoter" promoter 5160..5517 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5552..6346 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6578..6625 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6954..7542 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atgctccagc aggtcaatgg 661 ccacaatccg gggtctgatg gccaagccag ggagtacctc agagaagacc tgcaggagtt 721 cctgggtggg gaggtcctgc tgtacaaact ggatgacctc accagggtga atcctgtgac 781 actagagaca gtcctgaggt gcctgcaggc ccggtacatg gcagacacat tctacaccaa 841 tgctggctgc accctggtag ccttgaaccc cttcaagcct gttcctcagc tctactcgcc 901 cgagctaatg agagagtacc atgctgcgcc tcagccccag aaactgaagc cccatgtgtt 961 cactgtgggt gaacagacct acaggaatgt caagagcctg attgaaccag tcaaccagtc 1021 tattgttgtc agtggagaga gtggtgctgg aaagacatgg acgtctcgct gcctaatgaa 1081 gttctatgct gtggtggcca cctcacctgc atcttgggag agccacaaga ttgcagagag 1141 gatagaacag aggatcctga actccaaccc tgtcatggaa gcttttggga atgcgtgtac 1201 actgaggaat aacaacagca gtcgctttgg gaagttcatc cagctccagc tgaacagggc 1261 tcagcaaatg actggagccg cagtccagac ctacctccta gagaaaactc gagtggcctg 1321 ccaggcttcc agtgagagga acttccacat cttctatcag atttgcaaag gagccagtga 1381 ggacgagagg ctccagtggc accttcctga gggagctgcc ttctcctggc tgcccaaccc 1441 agagaggagc ttagaagagg attgttttga ggtgaccaga gaggccatgc tccatttggg 1501 cattgacacc cctacccaga acaacatctt taaggtccta gctggactgc tgcaccttgg 1561 caatatccag tttgctgcct ccgaggatga agcccagccc tgccagccga tggatgatgc 1621 caagtactct gtcaggacgg cagcctcgct gctggggctc ccagaggacg tgctgctgga 1681 gatggtgcag attagaacca tcagggcagg cagacagcag caggtgttcc ggaagccctg 1741 cgcccgagcc gagtgtgaca cccgtagaga ctgcctggcc aaactgatct atgcgcggtt 1801 gtttgactgg ctggtatcag tgatcaacag cagcatctgt gcagacaccg actcgtggac 1861 cactttcata ggcctgctgg atgtgtatgg atttgaatca tttcctgaca acagtctgga 1921 acagttgtgc atcaactacg ccaatgagaa gctgcagcag cattttgtgg ctcactacct 1981 aagggcccag caggaggaat acgcagttga gggcctggag tggtcattca tcaactacca 2041 ggacaaccag ccctgtttgg atctcattga gggaagcccc atcagcatct gctccctcat 2101 aaatgaggaa tgccgcctca atcgacccag cagcgcagcc cagctccaga cacgcattga 2161 gactgccctg gcaggcagcc cctgcctggg ccacaataag ctcagccggg agcccagctt 2221 cattgtggtg cattatgcgg ggcctgtgcg gtaccacaca gcaggcctgg tggagaagaa 2281 caaggaccct atcccacctg agctgaccag gctcctgcag caatcccagg accccctgct 2341 catggggctg tttcctacta accccaaaga gaagacccag gaggaacccc ctggccagag 2401 cagggcccct gtgttgaccg tggtgtccaa gttcaaggcc tcactggagc agcttctgca 2461 ggtcctacac agcaccacgc cccactacat tcgctgcatc aagcccaaca gccagggcca 2521 ggcgcagacc tttctccaag aggaggtcct gagccagctg gaggcctgtg gcctcgtgga 2581 gaccatccat atcagtgctg ctggcttccc catccgggtc tctcaccgaa actttgtaga 2641 acgatacaag ttactaagaa ggcttcatcc ttgcacatcc tctggccccg acagcccata 2701 tcctgccaaa gggctccctg aatggtgtcc acacagcgag gaagccacgc ttgaacctct 2761 catccaggac attctccaca ctctgccggt cctaactcag gcagcagcca taactggtga 2821 ctcggctgag gccatgccag cccccatgca ctgtggcagg accaaggtgt tcatgactga 2881 ctctatgctg gagcttctgg aatgtgggcg tgcccgggtg ctggagcagt gtgcccgctg 2941 catccagggt ggctggaggc gacaccggca ccgagagcag gagcggcagt ggcgggccgt 3001 catgctcatc caggcagcca ttcgttcctg gttaactcgg aaacacatcc agaggctgca 3061 tgcagctgcc acagtcatca agcgtgcatg gcagaagtgg agaatcagaa tggcctgcct 3121 tgctgctaaa gagctggatg gtgtggaaga aaaacacttc tctcaagctc cctgttccct 3181 gagcacctcg ccgctgcaga ccaggctcct ggaggcaata atccgcctct ggcccctggg 3241 actggtcctg gccaatacgg ctatgggtgt aggcagcttt cagaggaaat tagtggtctg 3301 ggcttgcctc cagctcccca ggggcagccc cagtagctac actgtccaga cagcacaaga 3361 ccaggctggt gtcacgtcca tccgagcgct gcctcaggga tcgataaagt ttcactgcag 3421 aaagtctcca ctgcggtatg ctgacatctg ccctgaacct tcaccctaca gcattacagg 3481 ctttaatcag attctgctgg aaagacacag gctgatccac gtgacctctt ctgccttcac 3541 tgggctgggg ggtggaggcg gatcaggagg cggtggatct gcggatccac cggtcgccac 3601 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 3661 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 3721 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 3781 cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa 3841 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 3901 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 3961 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 4021 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 4081 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 4141 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 4201 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 4261 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 4321 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 4381 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 4441 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 4501 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 4561 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 4621 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 4681 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 4741 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 4801 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 4861 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 4921 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 4981 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt 5041 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 5101 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc 5161 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 5221 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 5281 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 5341 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 5401 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 5461 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 5521 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 5581 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 5641 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 5701 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 5761 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 5821 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 5881 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 5941 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 6001 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 6061 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 6121 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 6181 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 6241 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 6301 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 6361 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 6421 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 6481 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 6541 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 6601 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 6661 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 6721 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 6781 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 6841 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 6901 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 6961 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 7021 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 7081 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 7141 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 7201 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 7261 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 7321 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 7381 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 7441 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 7501 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 7561 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 7621 cctgattctg tggataaccg tattaccgcc atgcat //