LOCUS       V043305                 7656 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043305
VERSION     V043305
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..3550
                     /label="MYO19(NM_001163735)"
                     /note="MYO19(NM_001163735)"
                     /gene="MYO19"
     CDS             3602..4321
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EYFP"
     polyA_signal    4444..4565
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4572..5027)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5054..5158
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5160..5517
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5552..6346
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6578..6625
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6954..7542
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgctccagc aggtcaatgg
      661 ccacaatccg gggtctgatg gccaagccag ggagtacctc agagaagacc tgcaggagtt
      721 cctgggtggg gaggtcctgc tgtacaaact ggatgacctc accagggtga atcctgtgac
      781 actagagaca gtcctgaggt gcctgcaggc ccggtacatg gcagacacat tctacaccaa
      841 tgctggctgc accctggtag ccttgaaccc cttcaagcct gttcctcagc tctactcgcc
      901 cgagctaatg agagagtacc atgctgcgcc tcagccccag aaactgaagc cccatgtgtt
      961 cactgtgggt gaacagacct acaggaatgt caagagcctg attgaaccag tcaaccagtc
     1021 tattgttgtc agtggagaga gtggtgctgg aaagacatgg acgtctcgct gcctaatgaa
     1081 gttctatgct gtggtggcca cctcacctgc atcttgggag agccacaaga ttgcagagag
     1141 gatagaacag aggatcctga actccaaccc tgtcatggaa gcttttggga atgcgtgtac
     1201 actgaggaat aacaacagca gtcgctttgg gaagttcatc cagctccagc tgaacagggc
     1261 tcagcaaatg actggagccg cagtccagac ctacctccta gagaaaactc gagtggcctg
     1321 ccaggcttcc agtgagagga acttccacat cttctatcag atttgcaaag gagccagtga
     1381 ggacgagagg ctccagtggc accttcctga gggagctgcc ttctcctggc tgcccaaccc
     1441 agagaggagc ttagaagagg attgttttga ggtgaccaga gaggccatgc tccatttggg
     1501 cattgacacc cctacccaga acaacatctt taaggtccta gctggactgc tgcaccttgg
     1561 caatatccag tttgctgcct ccgaggatga agcccagccc tgccagccga tggatgatgc
     1621 caagtactct gtcaggacgg cagcctcgct gctggggctc ccagaggacg tgctgctgga
     1681 gatggtgcag attagaacca tcagggcagg cagacagcag caggtgttcc ggaagccctg
     1741 cgcccgagcc gagtgtgaca cccgtagaga ctgcctggcc aaactgatct atgcgcggtt
     1801 gtttgactgg ctggtatcag tgatcaacag cagcatctgt gcagacaccg actcgtggac
     1861 cactttcata ggcctgctgg atgtgtatgg atttgaatca tttcctgaca acagtctgga
     1921 acagttgtgc atcaactacg ccaatgagaa gctgcagcag cattttgtgg ctcactacct
     1981 aagggcccag caggaggaat acgcagttga gggcctggag tggtcattca tcaactacca
     2041 ggacaaccag ccctgtttgg atctcattga gggaagcccc atcagcatct gctccctcat
     2101 aaatgaggaa tgccgcctca atcgacccag cagcgcagcc cagctccaga cacgcattga
     2161 gactgccctg gcaggcagcc cctgcctggg ccacaataag ctcagccggg agcccagctt
     2221 cattgtggtg cattatgcgg ggcctgtgcg gtaccacaca gcaggcctgg tggagaagaa
     2281 caaggaccct atcccacctg agctgaccag gctcctgcag caatcccagg accccctgct
     2341 catggggctg tttcctacta accccaaaga gaagacccag gaggaacccc ctggccagag
     2401 cagggcccct gtgttgaccg tggtgtccaa gttcaaggcc tcactggagc agcttctgca
     2461 ggtcctacac agcaccacgc cccactacat tcgctgcatc aagcccaaca gccagggcca
     2521 ggcgcagacc tttctccaag aggaggtcct gagccagctg gaggcctgtg gcctcgtgga
     2581 gaccatccat atcagtgctg ctggcttccc catccgggtc tctcaccgaa actttgtaga
     2641 acgatacaag ttactaagaa ggcttcatcc ttgcacatcc tctggccccg acagcccata
     2701 tcctgccaaa gggctccctg aatggtgtcc acacagcgag gaagccacgc ttgaacctct
     2761 catccaggac attctccaca ctctgccggt cctaactcag gcagcagcca taactggtga
     2821 ctcggctgag gccatgccag cccccatgca ctgtggcagg accaaggtgt tcatgactga
     2881 ctctatgctg gagcttctgg aatgtgggcg tgcccgggtg ctggagcagt gtgcccgctg
     2941 catccagggt ggctggaggc gacaccggca ccgagagcag gagcggcagt ggcgggccgt
     3001 catgctcatc caggcagcca ttcgttcctg gttaactcgg aaacacatcc agaggctgca
     3061 tgcagctgcc acagtcatca agcgtgcatg gcagaagtgg agaatcagaa tggcctgcct
     3121 tgctgctaaa gagctggatg gtgtggaaga aaaacacttc tctcaagctc cctgttccct
     3181 gagcacctcg ccgctgcaga ccaggctcct ggaggcaata atccgcctct ggcccctggg
     3241 actggtcctg gccaatacgg ctatgggtgt aggcagcttt cagaggaaat tagtggtctg
     3301 ggcttgcctc cagctcccca ggggcagccc cagtagctac actgtccaga cagcacaaga
     3361 ccaggctggt gtcacgtcca tccgagcgct gcctcaggga tcgataaagt ttcactgcag
     3421 aaagtctcca ctgcggtatg ctgacatctg ccctgaacct tcaccctaca gcattacagg
     3481 ctttaatcag attctgctgg aaagacacag gctgatccac gtgacctctt ctgccttcac
     3541 tgggctgggg ggtggaggcg gatcaggagg cggtggatct gcggatccac cggtcgccac
     3601 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     3661 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3721 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3781 cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
     3841 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3901 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3961 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     4021 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     4081 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     4141 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     4201 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     4261 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     4321 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     4381 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     4441 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4501 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4561 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4621 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4681 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4741 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4801 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4861 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4921 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4981 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     5041 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5101 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     5161 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5221 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5281 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5341 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5401 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5461 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     5521 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5581 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5641 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5701 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     5761 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     5821 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     5881 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     5941 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6001 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6061 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6121 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6181 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6241 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6301 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6361 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6421 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6481 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     6541 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     6601 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     6661 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     6721 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     6781 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     6841 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6901 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6961 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7021 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7081 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7141 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7201 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7261 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7321 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7381 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7441 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7501 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7561 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7621 cctgattctg tggataaccg tattaccgcc atgcat
//
