LOCUS V022325 6221 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022325 VERSION V022325 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 646..2157 /label="MYOC(NM_000261)" /note="MYOC(NM_000261)" /gene="MYOC" CDS 2167..2886 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 3009..3130 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3137..3592) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3619..3723 /gene="bla" /label="AmpR promoter" promoter 3725..4082 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4117..4911 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5143..5190 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5519..6107 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcccacca tggctatgag gttcttctgt 661 gcacgttgct gcagctttgg gcctgagatg ccagctgtcc agctgctgct tctggcctgc 721 ctggtgtggg atgtgggggc caggacagct cagctcagga aggccaatga ccagagtggc 781 cgatgccagt ataccttcag tgtggccagt cccaatgaat ccagctgccc agagcagagc 841 caggccatgt cagtcatcca taacttacag agagacagca gcacccaacg cttagacctg 901 gaggccacca aagctcgact cagctccctg gagagcctcc tccaccaatt gaccttggac 961 caggctgcca ggccccagga gacccaggag gggctgcaga gggagctggg caccctgagg 1021 cgggagcggg accagctgga aacccaaacc agagagttgg agactgccta cagcaacctc 1081 ctccgagaca agtcagttct ggaggaagag aagaagcgac taaggcaaga aaatgagaat 1141 ctggccagga ggttggaaag cagcagccag gaggtagcaa ggctgagaag gggccagtgt 1201 ccccagaccc gagacactgc tcgggctgtg ccaccaggct ccagagaagt ttctacgtgg 1261 aatttggaca ctttggcctt ccaggaactg aagtccgagc taactgaagt tcctgcttcc 1321 cgaattttga aggagagccc atctggctat ctcaggagtg gagagggaga caccggatgt 1381 ggagaactag tttgggtagg agagcctctc acgctgagaa cagcagaaac aattactggc 1441 aagtatggtg tgtggatgcg agaccccaag cccacctacc cctacaccca ggagaccacg 1501 tggagaatcg acacagttgg cacggatgtc cgccaggttt ttgagtatga cctcatcagc 1561 cagtttatgc agggctaccc ttctaaggtt cacatactgc ctaggccact ggaaagcacg 1621 ggtgctgtgg tgtactcggg gagcctctat ttccagggcg ctgagtccag aactgtcata 1681 agatatgagc tgaataccga gacagtgaag gctgagaagg aaatccctgg agctggctac 1741 cacggacagt tcccgtattc ttggggtggc tacacggaca ttgacttggc tgtggatgaa 1801 gcaggcctct gggtcattta cagcaccgat gaggccaaag gtgccattgt cctctccaaa 1861 ctgaacccag agaatctgga actcgaacaa acctgggaga caaacatccg taagcagtca 1921 gtcgccaatg ccttcatcat ctgtggcacc ttgtacaccg tcagcagcta cacctcagca 1981 gatgctaccg tcaactttgc ttatgacaca ggcacaggta tcagcaagac cctgaccatc 2041 ccattcaaga accgctataa gtacagcagc atgattgact acaaccccct ggagaagaag 2101 ctctttgcct gggacaactt gaacatggtc acttatgaca tcaagctctc caagatgcgg 2161 gatccaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 2221 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 2281 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 2341 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac 2401 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 2461 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 2521 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 2581 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 2641 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 2701 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 2761 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 2821 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 2881 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac 2941 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 3001 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 3061 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 3121 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt 3181 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca 3241 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta 3301 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta 3361 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg 3421 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga 3481 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg 3541 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc 3601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 3661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 3721 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc 3781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 3841 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 3901 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 3961 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 4021 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 4081 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 4141 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 4201 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 4261 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg 4321 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 4381 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 4441 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 4501 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 4561 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 4621 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat 4681 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 4741 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 4801 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 4861 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 4921 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 4981 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 5041 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg 5101 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga 5161 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc 5221 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt 5281 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg 5341 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt 5401 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 5461 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 5521 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 5581 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 5641 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 5701 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 5761 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 5821 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 5881 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 5941 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 6001 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 6061 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 6121 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 6181 tatcccctga ttctgtggat aaccgtatta ccgccatgca t //