LOCUS       V018289                 5542 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018289
VERSION     V018289
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        67..370
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        371..574
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        620..638
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     regulatory      673..682
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             682..705
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             739..1980
                     /label="Mbd2(NM_010773)"
                     /note="Mbd2(NM_010773)"
                     /gene="Mbd2"
     promoter        complement(2044..2062)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     polyA_signal    2336..2457
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2464..2919)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        2946..3050
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3052..3409
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3444..4238
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4470..4517
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4846..5434
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagagccc gggcggatcc
      721 cccgggctgc aggaattcat gcgcgcgcac ccggggggag gccgctgctg cccggagcag
      781 gaggaggggg agagcgcggc gggcggcagc ggcgctggcg gcgactccgc catagagcag
      841 gggggccagg gcagcgcgct cgctccgtcc ccggtgagcg gcgtgcgcag ggaaggcgct
      901 cggggcggcg gccgtggccg ggggcggtgg aagcaggcgg cccggggcgg cggcgtctgt
      961 ggccgtggcc gtggccgtgg ccggggtcgg ggccgtggcc ggggccgggg ccggggccgc
     1021 ggccgtcccc agagtggcgg cagcggcctt ggcggcgacg gcggcggcgg cgcgggcggc
     1081 tgcggcggcg gcagcggtgg cggcgtcgcc ccccggcggg atcctgtccc tttcccgtcg
     1141 gggagctcgg ggccggggcc caggggaccc cgggccacgg agagcgggaa gaggatggac
     1201 tgcccggccc tcccccccgg atggaagaag gaggaagtga tccgaaaatc agggctcagt
     1261 gctggcaaga gcgatgtcta ctacttcagt ccaagtggta agaagttcag aagtaaacct
     1321 cagctggcaa gatacctggg aaatgctgtt gaccttagca gttttgactt caggaccggc
     1381 aagatgatgc ctagtaaatt acagaagaac aagcagagac tccggaatga ccccctcaat
     1441 cagaacaagg gtaaaccaga cctgaacaca acattgccaa ttagacaaac tgcatcaatt
     1501 ttcaagcaac cagtaaccaa attcacgaac cacccgagca ataaggtgaa gtcagacccc
     1561 cagcggatga atgaacaacc acgtcagctt ttctgggaga agaggctaca aggacttagc
     1621 gcatcagatg taacagaaca aattataaaa accatggagc tacctaaagg tcttcaagga
     1681 gtcggtccag gtagcaatga cgagaccctt ctgtctgctg tggccagtgc tttacacaca
     1741 agctctgcgc ccatcacagg acaagtctct gctgccgtgg aaaagaaccc tgctgtttgg
     1801 cttaacacat ctcaacccct ctgcaaagct ttcattgtta cagatgaaga cattaggaaa
     1861 caggaagagc gagtccaaca agtacgcaag aaactggagg aggcactgat ggccgacatc
     1921 ctgtcccggg ctgcggacac ggaggaagta gacattgaca tggacagtgg agatgaggcg
     1981 taactcgagg gggggcccgg taccttaatt aattaaggta ccaggtaagt gtacccaatt
     2041 cgccctatag tgagtcgtat tacaattcac tcgatcgccc ttcccaacag ttgcgcagcc
     2101 tgaatggcga atggagatcc aatttttaag tgtataatgt gttaaactac tgattctaat
     2161 tgtttgtgta ttttagattc acagtcccaa ggctcatttc aggcccctca gtcctcacag
     2221 tctgttcatg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     2281 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     2341 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2401 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg
     2461 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     2521 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     2581 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     2641 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     2701 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     2761 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     2821 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     2881 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     2941 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3001 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat cctgaggcgg
     3061 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     3121 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     3181 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     3241 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3301 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3361 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     3421 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     3481 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     3541 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     3601 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     3661 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     3721 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     3781 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     3841 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     3901 tcgatcagga tgatctggac gaagaacatc aggggctcgc gccagccgaa ctgttcgcca
     3961 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     4021 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     4081 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagaacttg
     4141 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     4201 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     4261 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     4321 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     4381 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     4441 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     4501 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     4561 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     4621 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     4681 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4741 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4801 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4861 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4921 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4981 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5041 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5101 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5161 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5221 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5281 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5341 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5401 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5461 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5521 tgtggataac cgtattaccg cc
//
