LOCUS V038486 5016 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038486 VERSION V038486 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..952 /label="PCBD1(NM_000281)" /note="PCBD1(NM_000281)" /gene="PCBD1" CDS 1004..1681 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Strongin et al., 2007)" /transl_table=1 /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS GSQ*" /label="DsRed-Monomer" polyA_signal 1804..1925 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(1932..2387) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2414..2518 /gene="bla" /label="AmpR promoter" promoter 2520..2877 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2912..3706 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3938..3985 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4314..4902 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atggctggca aagcacacag 661 gctgagcgct gaggagaggg accagctgct gccaaacctg agggctgtgg ggtggaatga 721 gctggaaggc cgtgatgcca tcttcaagca gtttcatttc aaagacttca acagggcctt 781 tgggttcatg acaagagtgg ccctgcaggc tgagaaactg gaccaccatc ctgaatggtt 841 taacgtgtac aacaaggtcc acatcacgct gagcacccat gagtgtgccg gcctttcaga 901 acgggacata aacctggcca gcttcatcga acaagtagca gtgtccatga caggcggcgg 961 agggagtgga ggagggggaa gcgcggatcc accggtcgcc accatggaca acaccgagga 1021 cgtcatcaag gagttcatgc agttcaaggt gcgcatggag ggctccgtga acggccacta 1081 cttcgagatc gagggcgagg gcgagggcaa gccctacgag ggcacccaga ccgccaagct 1141 gcaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca 1201 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactaca tgaagctgtc 1261 cttccccgag ggcttcacct gggagcgctc catgaacttc gaggacggcg gcgtggtgga 1321 ggtgcagcag gactcctccc tgcaggacgg caccttcatc tacaaggtga agttcaaggg 1381 cgtgaacttc cccgccgacg gccccgtaat gcagaagaag actgccggct gggagccctc 1441 caccgagaag ctgtaccccc aggacggcgt gctgaagggc gagatctccc acgccctgaa 1501 gctgaaggac ggcggccact acacctgcga cttcaagacc gtgtacaagg ccaagaagcc 1561 cgtgcagctg cccggcaacc actacgtgga ctccaagctg gacatcacca accacaacga 1621 ggactacacc gtggtggagc agtacgagca cgccgaggcc cgccactccg gctcccagta 1681 gagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 1741 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 1801 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 1861 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 1921 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt 2401 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 2461 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc 2521 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 2581 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 2641 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 2701 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 2761 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 2821 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 2881 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 2941 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 3001 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 3061 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 3121 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 3181 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 3241 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 3301 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 3361 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 3421 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 3481 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 3541 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 3601 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 3661 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 3721 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 3781 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 3841 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 3901 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 3961 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 4021 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 4081 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 4141 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 4201 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 4261 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 4321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 4381 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 4441 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 4501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 4561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 4621 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 4681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 4741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 4801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 4861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 4921 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 4981 cctgattctg tggataaccg tattaccgcc atgcat //