LOCUS       V038445                 6075 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038445
VERSION     V038445
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..2011
                     /label="PDLIM7(NM_005451)"
                     /note="PDLIM7(NM_005451)"
                     /gene="PDLIM7"
     CDS             2063..2740
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /transl_table=1
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ*"
                     /label="DsRed-Monomer"
     polyA_signal    2863..2984
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2991..3446)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3473..3577
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3579..3936
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3971..4765
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4997..5044
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5373..5961
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggattcct tcaaagtagt
      661 gctggagggg ccagcacctt ggggcttccg gctgcaaggg ggcaaggact tcaatgtgcc
      721 cctctccatt tcccggctca ctcctggggg caaagcggcg caggccggag tggccgtggg
      781 tgactgggtg ctgagcatcg atggcgagaa tgcgggtagc ctcacacaca tcgaagctca
      841 gaacaagatc cgggcctgcg gggagcgcct cagcctgggc ctcagcaggg cccagccggt
      901 tcagagcaaa ccgcagaagg cctccgcccc cgccgcggac cctccgcggt acacctttgc
      961 acccagcgtc tccctcaaca agacggcccg gccctttggg gcgcccccgc ccgctgacag
     1021 cgccccgcag cagaatggac agccgctccg accgctggtc ccagatgcca gcaagcagcg
     1081 gctgatggag aacacagagg actggcggcc gcggccgggg acaggccagt cgcgttcctt
     1141 ccgcatcctt gcccacctca caggcaccga gttcatgcaa gacccggatg aggagcacct
     1201 gaagaaatca agccaggtgc ccaggacaga agccccagcc ccagcctcat ctacacccca
     1261 ggagccctgg cctggcccta ccgcccccag ccctaccagc cgcccgccct gggctgtgga
     1321 ccctgcgttt gccgagcgct atgccccgga caaaacgagc acagtgctga cccggcacag
     1381 ccagccggcc acgcccacgc cgctgcagag ccgcacctcc attgtgcagg cagctgccgg
     1441 aggggtgcca ggagggggca gcaacaacgg caagactccc gtgtgtcacc agtgccacaa
     1501 ggtcatccgg ggccgctacc tggtggcgct gggccacgcg taccacccgg aggagtttgt
     1561 gtgtagccag tgtgggaagg tcctggaaga gggtggcttc tttgaggaga agggcgccat
     1621 cttctgccca ccatgctatg acgtgcgcta tgcacccagc tgtgccaagt gcaagaagaa
     1681 gattacaggc gagatcatgc acgccctgaa gatgacctgg cacgtgcact gctttacctg
     1741 tgctgcctgc aagacgccca tccggaacag ggccttctac atggaggagg gcgtgcccta
     1801 ttgcgagcga gactatgaga agatgtttgg cacgaaatgc catggctgtg acttcaagat
     1861 cgacgctggg gaccgcttcc tggaggccct gggcttcagc tggcatgaca cctgcttcgt
     1921 ctgtgcgata tgtcagatca acctggaagg aaagaccttc tactccaaga aggacaggcc
     1981 tctctgcaag agccatgcct tctctcatgt gggcggcgga gggagtggag gagggggaag
     2041 cgcggatcca ccggtcgcca ccatggacaa caccgaggac gtcatcaagg agttcatgca
     2101 gttcaaggtg cgcatggagg gctccgtgaa cggccactac ttcgagatcg agggcgaggg
     2161 cgagggcaag ccctacgagg gcacccagac cgccaagctg caggtgacca agggcggccc
     2221 cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca aggcctacgt
     2281 gaagcacccc gccgacatcc ccgactacat gaagctgtcc ttccccgagg gcttcacctg
     2341 ggagcgctcc atgaacttcg aggacggcgg cgtggtggag gtgcagcagg actcctccct
     2401 gcaggacggc accttcatct acaaggtgaa gttcaagggc gtgaacttcc ccgccgacgg
     2461 ccccgtaatg cagaagaaga ctgccggctg ggagccctcc accgagaagc tgtaccccca
     2521 ggacggcgtg ctgaagggcg agatctccca cgccctgaag ctgaaggacg gcggccacta
     2581 cacctgcgac ttcaagaccg tgtacaaggc caagaagccc gtgcagctgc ccggcaacca
     2641 ctacgtggac tccaagctgg acatcaccaa ccacaacgag gactacaccg tggtggagca
     2701 gtacgagcac gccgaggccc gccactccgg ctcccagtag agcggccgcg actctagatc
     2761 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     2821 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     2881 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2941 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag
     3001 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3061 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3121 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3181 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3241 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3301 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3361 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3421 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     3481 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3541 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     3601 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3661 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3721 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3781 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3841 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3901 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     3961 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4021 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4081 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4141 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     4201 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4261 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4321 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4381 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4441 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     4501 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     4561 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4621 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     4681 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4741 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     4801 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4861 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     4921 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     4981 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     5041 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     5101 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     5161 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     5221 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5281 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5341 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     5521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5761 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5881 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6001 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6061 attaccgcca tgcat
//
