LOCUS V038445 6075 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038445 VERSION V038445 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..2011 /label="PDLIM7(NM_005451)" /note="PDLIM7(NM_005451)" /gene="PDLIM7" CDS 2063..2740 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Strongin et al., 2007)" /transl_table=1 /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS GSQ*" /label="DsRed-Monomer" polyA_signal 2863..2984 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2991..3446) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3473..3577 /gene="bla" /label="AmpR promoter" promoter 3579..3936 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3971..4765 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4997..5044 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5373..5961 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atggattcct tcaaagtagt 661 gctggagggg ccagcacctt ggggcttccg gctgcaaggg ggcaaggact tcaatgtgcc 721 cctctccatt tcccggctca ctcctggggg caaagcggcg caggccggag tggccgtggg 781 tgactgggtg ctgagcatcg atggcgagaa tgcgggtagc ctcacacaca tcgaagctca 841 gaacaagatc cgggcctgcg gggagcgcct cagcctgggc ctcagcaggg cccagccggt 901 tcagagcaaa ccgcagaagg cctccgcccc cgccgcggac cctccgcggt acacctttgc 961 acccagcgtc tccctcaaca agacggcccg gccctttggg gcgcccccgc ccgctgacag 1021 cgccccgcag cagaatggac agccgctccg accgctggtc ccagatgcca gcaagcagcg 1081 gctgatggag aacacagagg actggcggcc gcggccgggg acaggccagt cgcgttcctt 1141 ccgcatcctt gcccacctca caggcaccga gttcatgcaa gacccggatg aggagcacct 1201 gaagaaatca agccaggtgc ccaggacaga agccccagcc ccagcctcat ctacacccca 1261 ggagccctgg cctggcccta ccgcccccag ccctaccagc cgcccgccct gggctgtgga 1321 ccctgcgttt gccgagcgct atgccccgga caaaacgagc acagtgctga cccggcacag 1381 ccagccggcc acgcccacgc cgctgcagag ccgcacctcc attgtgcagg cagctgccgg 1441 aggggtgcca ggagggggca gcaacaacgg caagactccc gtgtgtcacc agtgccacaa 1501 ggtcatccgg ggccgctacc tggtggcgct gggccacgcg taccacccgg aggagtttgt 1561 gtgtagccag tgtgggaagg tcctggaaga gggtggcttc tttgaggaga agggcgccat 1621 cttctgccca ccatgctatg acgtgcgcta tgcacccagc tgtgccaagt gcaagaagaa 1681 gattacaggc gagatcatgc acgccctgaa gatgacctgg cacgtgcact gctttacctg 1741 tgctgcctgc aagacgccca tccggaacag ggccttctac atggaggagg gcgtgcccta 1801 ttgcgagcga gactatgaga agatgtttgg cacgaaatgc catggctgtg acttcaagat 1861 cgacgctggg gaccgcttcc tggaggccct gggcttcagc tggcatgaca cctgcttcgt 1921 ctgtgcgata tgtcagatca acctggaagg aaagaccttc tactccaaga aggacaggcc 1981 tctctgcaag agccatgcct tctctcatgt gggcggcgga gggagtggag gagggggaag 2041 cgcggatcca ccggtcgcca ccatggacaa caccgaggac gtcatcaagg agttcatgca 2101 gttcaaggtg cgcatggagg gctccgtgaa cggccactac ttcgagatcg agggcgaggg 2161 cgagggcaag ccctacgagg gcacccagac cgccaagctg caggtgacca agggcggccc 2221 cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca aggcctacgt 2281 gaagcacccc gccgacatcc ccgactacat gaagctgtcc ttccccgagg gcttcacctg 2341 ggagcgctcc atgaacttcg aggacggcgg cgtggtggag gtgcagcagg actcctccct 2401 gcaggacggc accttcatct acaaggtgaa gttcaagggc gtgaacttcc ccgccgacgg 2461 ccccgtaatg cagaagaaga ctgccggctg ggagccctcc accgagaagc tgtaccccca 2521 ggacggcgtg ctgaagggcg agatctccca cgccctgaag ctgaaggacg gcggccacta 2581 cacctgcgac ttcaagaccg tgtacaaggc caagaagccc gtgcagctgc ccggcaacca 2641 ctacgtggac tccaagctgg acatcaccaa ccacaacgag gactacaccg tggtggagca 2701 gtacgagcac gccgaggccc gccactccgg ctcccagtag agcggccgcg actctagatc 2761 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc 2821 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct 2881 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 2941 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag 3001 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca 3061 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag 3121 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 3181 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 3241 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 3301 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 3361 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 3421 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac 3481 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 3541 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg 3601 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3661 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 3721 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 3781 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 3841 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 3901 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg 3961 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 4021 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 4081 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 4141 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 4201 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 4261 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 4321 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 4381 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 4441 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag 4501 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 4561 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4621 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4681 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4741 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 4801 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 4861 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 4921 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa 4981 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt 5041 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc 5101 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt 5161 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac 5221 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 5281 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 5341 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 5401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 5461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 5521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 5581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 5641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 5701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 5761 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 5821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 5881 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 5941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 6001 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 6061 attaccgcca tgcat //