LOCUS       V046769                 6180 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046769
VERSION     V046769
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..2068
                     /label="RAB3IP(NM_175623)"
                     /note="RAB3IP(NM_175623)"
                     /gene="RAB3IP"
     CDS             2126..2845
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EYFP"
     polyA_signal    2968..3089
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3096..3551)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3578..3682
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3684..4041
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4076..4870
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5102..5149
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5478..6066
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgggattaa aaaagatgaa
      661 agggttatct tatgatgagg cttttgctat ggctaatgat cccttggaag gcttccatga
      721 agtaaacctt gcttcaccta cttctccgga ccttcttggt gtgtatgaat caggaactca
      781 agagcagact acctcaccaa gtgtcatcta ccggccacac ccttcagctt tatcctctgt
      841 acctatccag gcaaatgcat tagatgtttc tgaacttcct acacaacccg tgtattcatc
      901 ccccagacgt ttaaattgtg cggaaatatc tagtatcagc tttcatgtta cagacccagc
      961 cccttgctct acctctggag tcacagctgg attaactaaa ttaactacaa gaaaggacaa
     1021 ctataatgca gagagagagt ttttacaggg tgctactata acagaggctt gcgatggcag
     1081 tgatgatatt tttgggttga gtactgatag tctgtctcgt ttacgaagcc catctgtttt
     1141 ggaagttaga gaaaagggct atgaacgatt aaaagaagaa ctcgcaaaag ctcagaggga
     1201 actgaagtta aaagatgaag aatgtgagag gctttcaaaa gtgcgagatc aacttggaca
     1261 ggaattggaa gaactcacag ctagtctatt tgaggaagct cataaaatgg tgagagaagc
     1321 aaatatcaag caggcaacag cagaaaaaca gctaaaagaa gcacaaggaa aaattgatgt
     1381 acttcaagct gaagtagctg cattgaagac acttgtattg tccagttctc caacatcacc
     1441 tacgcaggag cctttgccag gtggaaagac accttttaaa aaggggcata caagaaataa
     1501 aagcacaagc agtgctatga gtggcagtca tcaggacctc agtgtgatac agccaattgt
     1561 aaaagactgc aaagaggctg acttatcctt gtataatgaa ttccgattgt ggaaggatga
     1621 gcccacaatg gacaggacgt gtcctttctt agacaaaatc taccaggaag atatctttcc
     1681 atgtttaaca ttctcaaaaa gtgagttggc ttcagctgtt ctggaggctg tggaaaacaa
     1741 tactctaagc attgaaccag tgggattaca acctatccgg tttgtgaaag cttctgcagt
     1801 tgaatgcgga ggaccaaaaa aatgtgctct cactggccag agtaagtcct gtaaacacag
     1861 aattaaatta ggggactcaa gcaactatta ttatatttct cctttttgca gatacaggat
     1921 cacttctgta tgtaactttt ttacatacat tcgatacatt cagcagggac tcgtgaaaca
     1981 gcaggatgtt gatcagatgt tttgggaggt tatgcagttg agaaaagaga tgtcattggc
     2041 aaagctgggt tatttcaaag aggaactcga attcggtgga ggcggatcag gaggcggtgg
     2101 atctccggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     2161 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2221 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2281 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt
     2341 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2401 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2461 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2521 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2581 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     2641 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2701 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     2761 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2821 cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc
     2881 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     2941 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     3001 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3061 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
     3121 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     3181 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     3241 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     3301 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     3361 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     3421 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     3481 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     3541 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3661 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     3721 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3781 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3841 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3901 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3961 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4021 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     4081 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     4141 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     4201 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     4261 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4321 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4381 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4441 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4501 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4561 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     4621 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4681 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4741 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4801 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4861 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     4921 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     4981 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     5041 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     5101 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     5161 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     5221 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     5281 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     5341 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5401 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5461 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5521 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5581 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5641 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5701 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5761 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5821 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5881 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5941 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6001 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6061 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6121 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
