LOCUS       V024106                 6771 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024106
VERSION     V024106
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..2665
                     /label="Tmem232(NM_001008973)"
                     /note="Tmem232(NM_001008973)"
                     /gene="Tmem232"
     CDS             2717..3436
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3559..3680
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3687..4142)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4169..4273
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4275..4632
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4667..5461
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5693..5740
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6069..6657
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgaaagctt ataagccacc
      661 tgtggtcaat aagtttggag taatatcttc cacttatcat gaagaactcc tgaaatctat
      721 ttttgaaagt tcaaacagaa gaaagagtca aaaaccaaag ccatcatttt caatttccaa
      781 agaattcata ttgagattca accacacaga taatccagca gaggaggaag aactgttgga
      841 acaagccagg agactcatcg tcaggtctaa gaggaagttg ggcctcaaga cactgggctc
      901 tgggaagcat gtgcatcttc cgaccgcatg ggctgaggtc atctatctgg ctcagtgcaa
      961 aggggagatc caagatgaag ctttaaatat gctccacgcc tccttggacc atgtctcctt
     1021 tgatcacgat cagcttcctg ccctgttttt tctcgcggaa tccgttttat atcgactctg
     1081 ttgcgatgca ttcatgaagg gatatttata ttcagttgag ataaaactgg tgaagattgg
     1141 ctatttgatc ttcttacggc tttttgtatt cttcctgcat ggtcacttag aaagctttaa
     1201 acaacatttg cttaggctgc agccatattt atacgcactt catttctcgg aaccgtccta
     1261 ctacaagtat ccgaacatca tctccaacgt gcagttcatc ctgaaaacat cggaaattat
     1321 ctgtaaaaga gagcttcatt ctgaaccatt cgtggaaagc ccagatgaaa ctgaggatcc
     1381 atattctgac ctgaatcatt tgcagctgaa taagaggggc tacgaagtca accacctgct
     1441 ctggcacagt gtggccgcct ggtcctgcgt tcagaacaat cgtcctcagc tcaccgaagt
     1501 gctggaacac ctcctcttct acaaaacgca gcttcagacg aaatgctggt tggattcagc
     1561 cctggctttg atggtcctgg gggaagctgc taagctggac atggcctgct tgaaaacact
     1621 gatggaccta gtgacagatt tccttgagaa catcttgtct gctcagaacc aggaagagaa
     1681 ctataatata tatgatactt cctgggcctc agagattgta ttcacatata caacaattat
     1741 tgcagaagtc tgtctgtatg cagctacctc tgacctgagg aaaacagctt tgattgggtt
     1801 ctgtgcctgt aaaagtccgc agcaaggtat ttccctcaca gacaagtctg aagagctgcc
     1861 agagttggat ggcgcaagca ttttaactct tctgaagtat ttctcttcca gaatctctga
     1921 taactgtgag aaggtgatct ggattggata ctatggcata gtgtataacc tggtgaagat
     1981 gtcatgggaa cttcagggtg agcaggatca ggatggactt agaaacatga tatggcaaac
     2041 actgcaaaag ataaaggatt atgagcaaga cccacggatc cgatgtgcat tagttatagc
     2101 ccaggccgaa ttaaatggcc catctgaccc tttctgcaca aaggccacac caaactcagg
     2161 agaagaagtt ttctccaagt acatcgggtg gagaatcgct actactctct ccagactctt
     2221 tttcccctct ctcgatgttg cccctccaaa gacgccagtg gaagtggacc tgccaagaaa
     2281 acacaccatc agagaacgcc agcctgcaaa aaagagagtt ctgcgcttta tcttgaagga
     2341 tcattcttct gtggtagaag tttccatgac tccgtatcca aatttcttca ccaaggcaga
     2401 taagaaattg gaagaaatca ttgatcatca ttggcagaaa gatatggagg cccgaaagcg
     2461 agaagaggaa gcatataagg cccaaaatca gaaggacaag gaggaaaagg agaagattca
     2521 ctttcaagaa attatgaagc aaagagaacg gaaactgaac aaacaaacca aaccctatga
     2581 aataattctg tcagaaaaag aaagtggctc agaaaagaaa tgtgggttct tcgagctgaa
     2641 acccagcacc gcaccaaatg ctaagggcgg tggaggttca ggcggtggag gatctcagga
     2701 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2761 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2821 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2881 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2941 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3001 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3061 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3121 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3181 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3241 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3301 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     3361 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3421 cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     3481 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3541 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3601 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3661 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     3721 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3781 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3841 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3901 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3961 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     4021 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     4081 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     4141 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4201 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4261 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     4321 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4381 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4441 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4501 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4561 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4621 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4681 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4741 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4801 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4861 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4921 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4981 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5041 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5101 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5161 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     5221 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5281 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5341 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5401 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5461 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     5521 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5581 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     5641 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     5701 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     5761 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5821 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5881 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     5941 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6001 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6061 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6121 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6181 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6241 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6301 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6361 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6421 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6481 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6541 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6601 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6661 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6721 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
