LOCUS       V038446                 5634 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038446
VERSION     V038446
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..1570
                     /label="UPP1(NM_003364)"
                     /note="UPP1(NM_003364)"
                     /gene="UPP1"
     CDS             1622..2299
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /transl_table=1
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ*"
                     /label="DsRed-Monomer"
     polyA_signal    2422..2543
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2550..3005)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3032..3136
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3138..3495
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3530..4324
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4556..4603
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4932..5520
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggcggcca cgggagccaa
      661 tgcagagaaa gctgaaagtc acaatgattg ccccgtcaga cttttaaatc caaacatagc
      721 aaaaatgaaa gaagatattc tctatcattt caatctcacc actagcagac acaatttccc
      781 agccttgttt ggagatgtga agtttgtgtg tgttggtgga agcccctccc ggatgaaagc
      841 cttcatcagg tgcgttggtg cagagctggg ccttgactgc ccaggtagag actatcccaa
      901 catctgtgcg ggaactgacc gctatgccat gtataaagta ggaccggtgc tgtctgtcag
      961 tcatggtatg ggcattcctt ctatctcaat catgttgcat gagctcataa agctgctgta
     1021 ctatgcccgg tgctccaacg tcactatcat ccgcattggc acttctggtg ggataggtct
     1081 ggagcccggc actgtggtca taacagagca ggcagtggat acctgcttca aggcagagtt
     1141 tgagcagatt gtcctgggga agcgggtcat ccggaaaacg gaccttaaca agaagctggt
     1201 gcaggagctg ttgctgtgtt ctgcagagct gagcgagttc accacagtgg tggggaacac
     1261 catgtgcacc ttggacttct atgaagggca aggccgtctg gatggggctc tctgctccta
     1321 cacggagaag gacaagcagg cgtatctgga ggcagcctat gcagccggcg tccgcaatat
     1381 cgagatggag tcctcggtgt ttgccgccat gtgcagcgcc tgcggcctcc aagcggccgt
     1441 ggtgtgtgtc accctcctga accgcctgga aggggaccag atcagcagcc ctcgcaatgt
     1501 gctcagcgag taccagcaga ggccgcagcg gctggtgagc tacttcatca agaagaaact
     1561 gagcaaggcc ggcggcggag ggagtggagg agggggaagc gcggatccac cggtcgccac
     1621 catggacaac accgaggacg tcatcaagga gttcatgcag ttcaaggtgc gcatggaggg
     1681 ctccgtgaac ggccactact tcgagatcga gggcgagggc gagggcaagc cctacgaggg
     1741 cacccagacc gccaagctgc aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     1801 cctgtccccc cagttccagt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     1861 cgactacatg aagctgtcct tccccgaggg cttcacctgg gagcgctcca tgaacttcga
     1921 ggacggcggc gtggtggagg tgcagcagga ctcctccctg caggacggca ccttcatcta
     1981 caaggtgaag ttcaagggcg tgaacttccc cgccgacggc cccgtaatgc agaagaagac
     2041 tgccggctgg gagccctcca ccgagaagct gtacccccag gacggcgtgc tgaagggcga
     2101 gatctcccac gccctgaagc tgaaggacgg cggccactac acctgcgact tcaagaccgt
     2161 gtacaaggcc aagaagcccg tgcagctgcc cggcaaccac tacgtggact ccaagctgga
     2221 catcaccaac cacaacgagg actacaccgt ggtggagcag tacgagcacg ccgaggcccg
     2281 ccactccggc tcccagtaga gcggccgcga ctctagatca taatcagcca taccacattt
     2341 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2401 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     2461 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2521 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt
     2581 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     2641 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     2701 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     2761 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     2821 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     2881 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     2941 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     3001 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3061 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3121 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     3181 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3241 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3301 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     3361 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     3421 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     3481 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     3541 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     3601 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     3661 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     3721 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     3781 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     3841 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     3901 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     3961 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     4021 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     4081 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     4141 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     4201 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     4261 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     4321 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     4381 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4441 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     4501 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     4561 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     4621 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     4681 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     4741 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     4801 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4861 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4921 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4981 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5041 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5101 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5161 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5221 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5281 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5341 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5401 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5461 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5521 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5581 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
