LOCUS V038582 6163 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038582 VERSION V038582 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 925..1914 /label="EXTL2(NM_001439)" /note="EXTL2(NM_001439)" /gene="EXTL2" CDS 1927..1992 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2027..2251 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2297..2725 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2739..3068 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3080..3679 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 3839..3960 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4009..4025) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4033..4049 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4057..4087) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4102..4123 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4411..4996) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5167..6027) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6028..6132) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 aagctttcta gagaattcgc caccatgagg tgttgccaca tctgcaaact tcctgggaga 961 gtaatgggga ttcgagtgct tcgattatct ttggtggtca tcctcgtatt attactggta 1021 gctggtgctt tgactgcctt acttcccagt gttaaagaag acaagatgct catgttgcgt 1081 agggaaataa aatcccaggg caagtccacc atggactcct ttactctcat aatgcagacg 1141 tacaacagaa cagatctctt attgaaactt ttaaatcatt atcaggctgt accaaatctg 1201 cacaaagtga ttgtggtatg gaacaatatt ggagagaagg caccagatga attatggaat 1261 tctctagggc cccaccctat ccctgtgatc ttcaaacaac agacagcaaa caggatgaga 1321 aatcgactcc aggtctttcc tgaactggaa accaatgcag tgttgatggt agatgatgac 1381 acactcatca gcaccccaga ccttgttttt gctttctcag tttggcagca atttcctgat 1441 caaattgtag gatttgttcc tagaaagcac gtctctactt catcaggtat ctacagttat 1501 ggaagttttg aaatgcaagc accagggtct ggaaatggtg accagtactc tatggtgctg 1561 attggagcct cattcttcaa tagcaaatat cttgaattat ttcagaggca acctgcagct 1621 gtccatgctt tgatagatga tactcaaaac tgtgatgata ttgccatgaa ttttatcatt 1681 gccaagcata ttggcaagac ttcagggata tttgtgaagc ctgtaaacat ggacaatttg 1741 gaaaaagaaa ccaacagtgg ctattctgga atgtggcatc gagctgagca cgctctgcag 1801 aggtcttatt gtataaataa gcttgttaat atctatgata gcatgccctt aagatactcc 1861 aacattatga tttcccagtt tggttttcca tatgccaact acaaaagaaa aatactcgag 1921 accggtgact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat 1981 gacgatgaca agtgaccggt aagtttaaac cgctgatcag cctcgactgt gccttctagt 2041 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact 2101 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat 2161 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc 2221 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc 2281 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 2341 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 2401 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 2461 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 2521 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 2581 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 2641 tattcttttg atttataagg gattttggcc atttcggcct attggttaaa aaatgagctg 2701 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa 2761 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 2821 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 2881 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 2941 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 3001 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 3061 tttgcaaaaa gctcccggga tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 3121 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 3181 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 3241 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 3301 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 3361 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 3421 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 3481 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 3541 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 3601 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 3661 ccgcaagccc ggtgcctgat tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 3721 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 3781 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 3841 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 3901 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 3961 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 4021 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 4081 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 4141 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 4201 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 4261 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 4321 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 4381 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 4441 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 4501 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 4561 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 4621 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 4681 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 4741 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 4801 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 4861 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 4921 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag 4981 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 5041 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 5101 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 5161 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 5221 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 5281 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 5341 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 5401 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 5461 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 5521 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 5581 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 5641 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 5701 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 5761 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 5821 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 5881 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 5941 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 6001 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 6061 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 6121 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc //