LOCUS V043901 7252 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043901 VERSION V043901 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 925..3003 /label="GMPS(NM_003875)" /note="GMPS(NM_003875)" /gene="GMPS" CDS 3016..3081 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3116..3340 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3386..3814 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3828..4157 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4169..4768 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 4928..5049 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5098..5114) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5122..5138 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5146..5176) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5191..5212 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5500..6085) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6256..7116) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7117..7221) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 aagctttcta gagaattcgc caccatggct ctgtgcaacg gagactccaa gctggagaat 961 gctggaggag accttaagga tggccaccac cactatgaag gagctgttgt cattctggat 1021 gctggtgctc agtacgggaa agtcatagac cgaagagtga gggaactgtt cgtgcagtct 1081 gaaattttcc ccttggaaac accagcattt gctataaagg aacaaggatt ccgtgctatt 1141 atcatctctg gaggacctaa ttctgtgtat gctgaagatg ctccctggtt tgatccagca 1201 atattcacta ttggcaagcc tgttcttgga atttgctatg gtatgcagat gatgaataag 1261 gtatttggag gtactgtgca caaaaaaagt gtcagagaag atggagtttt caacattagt 1321 gtggataata catgttcatt attcaggggc cttcagaagg aagaagttgt tttgcttaca 1381 catggagata gtgtagacaa agtagctgat ggattcaagg ttgtggcacg ttctggaaac 1441 atagtagcag gcatagcaaa tgaatctaaa aagttatatg gagcacagtt ccaccctgaa 1501 gttggcctta cagaaaatgg aaaagtaata ctgaagaatt tcctttatga tatagctgga 1561 tgcagtggaa ccttcaccgt gcagaacaga gaacttgagt gtattcgaga gatcaaagag 1621 agagtaggca cgtcaaaagt tttggtttta ctcagtggtg gagtagactc aacagtttgt 1681 acagctttgc taaatcgtgc tttgaaccaa gaacaagtca ttgctgtgca cattgataat 1741 ggctttatga gaaaacgaga aagccagtct gttgaagagg ccctcaaaaa gcttggaatt 1801 caggtcaaag tgataaatgc tgctcattct ttctacaatg gaacaacaac cctaccaata 1861 tcagatgaag atagaacccc acggaaaaga attagcaaaa cgttaaatat gaccacaagt 1921 cctgaagaga aaagaaaaat cattggggat acttttgtta agattgccaa tgaagtaatt 1981 ggagaaatga acttgaaacc agaggaggtt ttccttgccc aaggtacttt acggcctgat 2041 ctaattgaaa gtgcatccct tgttgcaagt ggcaaagctg aactcatcaa aacccatcac 2101 aatgacacag agctcatcag aaagttgaga gaggagggaa aagtaataga acctctgaaa 2161 gattttcata aagatgaagt gagaattttg ggcagagaac ttggacttcc agaagagtta 2221 gtttccaggc atccatttcc aggtcctggc ctggcaatca gagtaatatg tgctgaagaa 2281 ccttatattt gtaaggactt tcctgaaacc aacaatattt tgaaaatagt agctgatttt 2341 tctgcaagtg ttaaaaagcc acatacccta ttacagagag tcaaagcctg cacaacagaa 2401 gaggatcagg agaagctgat gcaaattacc agtctgcatt cactgaatgc cttcttgctg 2461 ccaattaaaa ctgtaggtgt gcagggtgac tgtcgttcct acagttacgt gtgtggaatc 2521 tccagtaaag atgaacctga ctgggaatca cttatttttc tggctaggct tatacctcgc 2581 atgtgtcaca acgttaacag agttgtttat atatttggcc caccagttaa agaacctcct 2641 acagatgtta ctcccacttt cttgacaaca ggggtgctca gtactttacg ccaagctgat 2701 tttgaggccc ataacattct cagggagtct gggtatgctg ggaaaatcag ccagatgccg 2761 gtgattttga caccattaca ttttgatcgg gacccacttc aaaagcagcc ttcatgccag 2821 agatctgtgg ttattcgaac ctttattact agtgacttca tgactggtat acctgcaaca 2881 cctggcaatg agatccctgt agaggtggta ttaaagatgg tcactgagat taagaagatt 2941 cctggtattt ctcgaattat gtatgactta acatcaaagc ccccaggaac tactgagtgg 3001 gagctcgaga ccggtgacta caaagaccat gacggtgatt ataaagatca tgacatcgat 3061 tacaaggatg acgatgacaa gtgaccggta agtttaaacc gctgatcagc ctcgactgtg 3121 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 3181 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 3241 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 3301 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 3361 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 3421 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 3481 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 3541 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 3601 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 3661 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 3721 atctcggtct attcttttga tttataaggg attttggcca tttcggccta ttggttaaaa 3781 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 3841 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 3901 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 3961 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 4021 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 4081 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 4141 gcctaggctt ttgcaaaaag ctcccgggat gaccgagtac aagcccacgg tgcgcctcgc 4201 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 4261 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 4321 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 4381 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 4441 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 4501 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 4561 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 4621 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 4681 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 4741 gtgcatgacc cgcaagcccg gtgcctgatt cgaaatgacc gaccaagcga cgcccaacct 4801 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 4861 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 4921 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 4981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 5041 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 5101 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 5161 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 5221 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 5281 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 5341 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 5401 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 5461 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 5521 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 5581 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 5641 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 5701 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 5761 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 5821 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 5881 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 5941 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 6001 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat 6061 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 6121 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 6181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 6241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 6301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 6361 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 6421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 6481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 6541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 6601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 6661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 6721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 6781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 6841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 6901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 6961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 7021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 7081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 7141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 7201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc //